Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100
Objective: In radiation treatment, the irradiation which is effective enough to control the tumors far exceeds normal-tissues tolerance. Thus to avoid such unfavourable outcomes, some methods sensitizing the tumor cells to radiation are used. Iododeoxyuridine (IUdR) is a halogenated thymidine ana...
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2014-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_454558474334357742427452634246344662625712-Ali%20Neshasteh-Riz-.pdf |
| _version_ | 1818954830046035968 |
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| author | Ali Neshasteh-Riz Rozhin Rahdani Ahmad Mostaar |
| author_facet | Ali Neshasteh-Riz Rozhin Rahdani Ahmad Mostaar |
| author_sort | Ali Neshasteh-Riz |
| collection | DOAJ |
| description | Objective: In radiation treatment, the irradiation which is effective enough to control the
tumors far exceeds normal-tissues tolerance. Thus to avoid such unfavourable outcomes,
some methods sensitizing the tumor cells to radiation are used. Iododeoxyuridine (IUdR)
is a halogenated thymidine analogue that known to be effective as a radiosensitizer in human
cancer therapy. Improving the potential efficacy of radiation therapy after combining
to hyperthermia depends on the magnitude of the differential sensitization of the hyperthermic
effects or on the differential cytotoxicity of the radiation effects on the tumor cells.
In this study, we evaluated the combined effects of IUdR, hyperthermia and gamma rays
of 60Co on human glioblastoma spheroids culture.
Materials and Methods: In this experimental study,the cultured spheroids with 100μm diameter
were treated by 1 μM IUdR, 43˚C hyperthermia for an hour and 2 Gy gamma rays,
respectively. The DNA damages induced in cells were compared using alkaline comet
assay method, and dosimetry was then performed by TLD-100. Comet scores were calculated
as mean ± standard error of mean (SEM) using one-way ANOVA.
Results:Comparison of DNA damages induced by IUdR and hyperthermia + gamma treatment
showed 2.67- and 1.92-fold enhancement, respectively, as compared to the damages
induced by radiation alone or radiation combined IUdR. Dosimetry results showed
the accurate dose delivered to cells.
Conclusion: Analysis of the comet tail moments of spheroids showed that the radiation
treatments combined with hyperthermia and IUdR caused significant radiosensitization
when compared to related results of irradiation alone or of irradiation with IUdR. These results
suggest a potential clinical advantage of combining radiation with hyperthermia and
indicate effectiveness of hyperthermia treatment in inducing cytotoxicity of tumor cells. |
| first_indexed | 2024-12-20T10:28:24Z |
| format | Article |
| id | doaj.art-03896ff72e7c4a7b88c86a480d1f53d8 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-20T10:28:24Z |
| publishDate | 2014-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-03896ff72e7c4a7b88c86a480d1f53d82022-12-21T19:43:46ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142014-04-01163335342Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100Ali Neshasteh-Riz0Rozhin Rahdani1Ahmad Mostaar2Department of Radiobiology, Iran University of Allied Health, Tehran, IranDepartment of Nuclear Engineering, Science and Research Islamic Azad University, Tehran, IranDepartment of Medical Physics, Shahid Beheshti University of Medical Sciences, Tehran, IranObjective: In radiation treatment, the irradiation which is effective enough to control the tumors far exceeds normal-tissues tolerance. Thus to avoid such unfavourable outcomes, some methods sensitizing the tumor cells to radiation are used. Iododeoxyuridine (IUdR) is a halogenated thymidine analogue that known to be effective as a radiosensitizer in human cancer therapy. Improving the potential efficacy of radiation therapy after combining to hyperthermia depends on the magnitude of the differential sensitization of the hyperthermic effects or on the differential cytotoxicity of the radiation effects on the tumor cells. In this study, we evaluated the combined effects of IUdR, hyperthermia and gamma rays of 60Co on human glioblastoma spheroids culture. Materials and Methods: In this experimental study,the cultured spheroids with 100μm diameter were treated by 1 μM IUdR, 43˚C hyperthermia for an hour and 2 Gy gamma rays, respectively. The DNA damages induced in cells were compared using alkaline comet assay method, and dosimetry was then performed by TLD-100. Comet scores were calculated as mean ± standard error of mean (SEM) using one-way ANOVA. Results:Comparison of DNA damages induced by IUdR and hyperthermia + gamma treatment showed 2.67- and 1.92-fold enhancement, respectively, as compared to the damages induced by radiation alone or radiation combined IUdR. Dosimetry results showed the accurate dose delivered to cells. Conclusion: Analysis of the comet tail moments of spheroids showed that the radiation treatments combined with hyperthermia and IUdR caused significant radiosensitization when compared to related results of irradiation alone or of irradiation with IUdR. These results suggest a potential clinical advantage of combining radiation with hyperthermia and indicate effectiveness of hyperthermia treatment in inducing cytotoxicity of tumor cells.http://celljournal.org/library/upload/article/af_454558474334357742427452634246344662625712-Ali%20Neshasteh-Riz-.pdfRadiationHyperthermiaIUdRGlioblastoma |
| spellingShingle | Ali Neshasteh-Riz Rozhin Rahdani Ahmad Mostaar Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 Cell Journal Radiation Hyperthermia IUdR Glioblastoma |
| title | Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 |
| title_full | Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 |
| title_fullStr | Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 |
| title_full_unstemmed | Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 |
| title_short | Evaluation of The Combined Effects of Hyperthermia, Cobalt-60 Gamma Rays and IUdR on Cultured Glioblastoma Spheroid Cells and Dosimetry Using TLD-100 |
| title_sort | evaluation of the combined effects of hyperthermia cobalt 60 gamma rays and iudr on cultured glioblastoma spheroid cells and dosimetry using tld 100 |
| topic | Radiation Hyperthermia IUdR Glioblastoma |
| url | http://celljournal.org/library/upload/article/af_454558474334357742427452634246344662625712-Ali%20Neshasteh-Riz-.pdf |
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