Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients
ObjectiveThe primary objective of this study is to investigate the mechanism by which Bacillus coagulans TBC169 accelerates intestinal function recovery in patients who have undergone gynecological laparoscopic surgery, using metabolomics and gut microbiota analysis.MethodsA total of 20 subjects wer...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1284402/full |
| _version_ | 1797244815433269248 |
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| author | Weiqi Gao Weiqi Gao Ya Yan Ya Yan Zhaobo Guan Zhaobo Guan Jingmin Zhang Jingmin Zhang Weihong Chen Weihong Chen Weihong Chen |
| author_facet | Weiqi Gao Weiqi Gao Ya Yan Ya Yan Zhaobo Guan Zhaobo Guan Jingmin Zhang Jingmin Zhang Weihong Chen Weihong Chen Weihong Chen |
| author_sort | Weiqi Gao |
| collection | DOAJ |
| description | ObjectiveThe primary objective of this study is to investigate the mechanism by which Bacillus coagulans TBC169 accelerates intestinal function recovery in patients who have undergone gynecological laparoscopic surgery, using metabolomics and gut microbiota analysis.MethodsA total of 20 subjects were selected and randomly divided into two groups: the intervention group (n = 10) receiving Bacillus coagulans TBC169 Tablets (6 pills, 1.05 × 108 CFU), and the control group (n = 10) receiving placebos (6 pills). After the initial postoperative defecation, fecal samples were collected from each subject to analyze their gut microbiota and metabolic profiles by high-throughput 16S rRNA gene sequencing analysis and untargeted metabonomic.ResultsThere were no statistically significant differences observed in the α-diversity and β-diversity between the two groups; however, in the intervention group, there was a significant reduction in the relative abundance of unclassified_Enterobacteriaceae at the genus level. Furthermore, the control group showed increased levels of Holdemanella and Enterobacter, whereas the intervention group exhibited elevated levels of Intestinimonas. And administration of Bacillus coagulans TBC169 led to variations in 2 metabolic pathways: D-glutamine and D-glutamate metabolism, and arginine biosynthesis.ConclusionThis study demonstrated that consuming Bacillus coagulans TBC169 after gynecological laparoscopic surgery might inhibit the proliferation of harmful Enterobacteriaceae; mainly influence 2 pathways including D-glutamine and D-glutamate metabolism, and arginine biosynthesis; and regulate metabolites related to immunity and intestinal motility; which can help regulate immune function, maintain intestinal balance, promote intestinal peristalsis, and thus accelerate the recovery of intestinal function. |
| first_indexed | 2024-04-24T19:17:00Z |
| format | Article |
| id | doaj.art-039033ba4a2946ecbfd8f57dcb51e819 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T19:17:00Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-039033ba4a2946ecbfd8f57dcb51e8192024-03-26T04:49:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.12844021284402Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patientsWeiqi Gao0Weiqi Gao1Ya Yan2Ya Yan3Zhaobo Guan4Zhaobo Guan5Jingmin Zhang6Jingmin Zhang7Weihong Chen8Weihong Chen9Weihong Chen10Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, ChinaTongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, ChinaSchool of Pharmacy, Shanxi Medical University, Taiyuan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, ChinaSchool of Pharmacy, Shanxi Medical University, Taiyuan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, ChinaTongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, ChinaTongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSchool of Pharmacy, Shanxi Medical University, Taiyuan, ChinaObjectiveThe primary objective of this study is to investigate the mechanism by which Bacillus coagulans TBC169 accelerates intestinal function recovery in patients who have undergone gynecological laparoscopic surgery, using metabolomics and gut microbiota analysis.MethodsA total of 20 subjects were selected and randomly divided into two groups: the intervention group (n = 10) receiving Bacillus coagulans TBC169 Tablets (6 pills, 1.05 × 108 CFU), and the control group (n = 10) receiving placebos (6 pills). After the initial postoperative defecation, fecal samples were collected from each subject to analyze their gut microbiota and metabolic profiles by high-throughput 16S rRNA gene sequencing analysis and untargeted metabonomic.ResultsThere were no statistically significant differences observed in the α-diversity and β-diversity between the two groups; however, in the intervention group, there was a significant reduction in the relative abundance of unclassified_Enterobacteriaceae at the genus level. Furthermore, the control group showed increased levels of Holdemanella and Enterobacter, whereas the intervention group exhibited elevated levels of Intestinimonas. And administration of Bacillus coagulans TBC169 led to variations in 2 metabolic pathways: D-glutamine and D-glutamate metabolism, and arginine biosynthesis.ConclusionThis study demonstrated that consuming Bacillus coagulans TBC169 after gynecological laparoscopic surgery might inhibit the proliferation of harmful Enterobacteriaceae; mainly influence 2 pathways including D-glutamine and D-glutamate metabolism, and arginine biosynthesis; and regulate metabolites related to immunity and intestinal motility; which can help regulate immune function, maintain intestinal balance, promote intestinal peristalsis, and thus accelerate the recovery of intestinal function.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1284402/fullBacillus coagulansgynecological laparoscopic surgeryintestinal functiongut microbiotametabolite |
| spellingShingle | Weiqi Gao Weiqi Gao Ya Yan Ya Yan Zhaobo Guan Zhaobo Guan Jingmin Zhang Jingmin Zhang Weihong Chen Weihong Chen Weihong Chen Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients Frontiers in Microbiology Bacillus coagulans gynecological laparoscopic surgery intestinal function gut microbiota metabolite |
| title | Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| title_full | Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| title_fullStr | Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| title_full_unstemmed | Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| title_short | Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| title_sort | effects of bacillus coagulans tbc169 on gut microbiota and metabolites in gynecological laparoscopy patients |
| topic | Bacillus coagulans gynecological laparoscopic surgery intestinal function gut microbiota metabolite |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1284402/full |
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