Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings
Abstract Background Seaweeds are a viable bioresource for suffering plants against salt stress, as they abundant in nutrients, hormones, vitamins, secondary metabolites, and many other phytochemicals that sustain plants' growth under both typical and stressful situations. The alleviating capaci...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-03-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-023-00483-z |
| _version_ | 1797863845470928896 |
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| author | Marwa M. Hamouda Abdelfattah Badr Sameh S. Ali Alia M. Adham Hanan I. Sayed Ahmed Khalil M. Saad-Allah |
| author_facet | Marwa M. Hamouda Abdelfattah Badr Sameh S. Ali Alia M. Adham Hanan I. Sayed Ahmed Khalil M. Saad-Allah |
| author_sort | Marwa M. Hamouda |
| collection | DOAJ |
| description | Abstract Background Seaweeds are a viable bioresource for suffering plants against salt stress, as they abundant in nutrients, hormones, vitamins, secondary metabolites, and many other phytochemicals that sustain plants' growth under both typical and stressful situations. The alleviating capacity of extracts from three brown algae (Sargassum vulgare, Colpomenia sinuosa, and Pandia pavonica) in pea (Pisum sativum L.) was investigated in this study. Methods Pea seeds were primed for 2 h either with seaweed extracts (SWEs) or distilled water. Seeds were then subjected to salinity levels of 0.0, 50, 100, and 150 mM NaCl. On the 21st day, seedlings were harvested for growth, physiological and molecular investigations. Results SWEs helped reduce the adverse effects of salinity on pea, with S. vulgare extract being the most effective. Furthermore, SWEs diminished the effect of NaCl-salinity on germination, growth rate, and pigment content and raised the osmolytes proline and glycine betaine levels. On the molecular level, two low-molecular-weight proteins were newly synthesized by the NaCl treatments and three by priming pea seeds with SWEs. The number of inter-simple sequence repeats (ISSR) markers increased from 20 in the control to 36 in 150 mM NaCl-treated seedlings, including four unique markers. Priming with SWEs triggered more markers than the control, however about ten of the salinity-induced markers were not detected following seed priming before NaCl treatments. By priming with SWEs, seven unique markers were elicited. Conclusion All in all, priming with SWEs alleviated salinity stress on pea seedlings. Salinity-responsive proteins and ISSR markers are produced in response to salt stress and priming with SWEs. |
| first_indexed | 2024-04-09T22:42:11Z |
| format | Article |
| id | doaj.art-039562bad6424d8eb956ee7c1659cae9 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-09T22:42:11Z |
| publishDate | 2023-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-039562bad6424d8eb956ee7c1659cae92023-03-22T12:02:36ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202023-03-0121111510.1186/s43141-023-00483-zGrowth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlingsMarwa M. Hamouda0Abdelfattah Badr1Sameh S. Ali2Alia M. Adham3Hanan I. Sayed Ahmed4Khalil M. Saad-Allah5Botany Department, Faculty of Science, Tanta UniversityBotany and Microbiology Department, Faculty of Science, Helwan UniversityBotany Department, Faculty of Science, Tanta UniversityPlant Protection Department, Faculty of Agriculture, University of AL-MuthannaBotany Department, Faculty of Science, Tanta UniversityBotany Department, Faculty of Science, Tanta UniversityAbstract Background Seaweeds are a viable bioresource for suffering plants against salt stress, as they abundant in nutrients, hormones, vitamins, secondary metabolites, and many other phytochemicals that sustain plants' growth under both typical and stressful situations. The alleviating capacity of extracts from three brown algae (Sargassum vulgare, Colpomenia sinuosa, and Pandia pavonica) in pea (Pisum sativum L.) was investigated in this study. Methods Pea seeds were primed for 2 h either with seaweed extracts (SWEs) or distilled water. Seeds were then subjected to salinity levels of 0.0, 50, 100, and 150 mM NaCl. On the 21st day, seedlings were harvested for growth, physiological and molecular investigations. Results SWEs helped reduce the adverse effects of salinity on pea, with S. vulgare extract being the most effective. Furthermore, SWEs diminished the effect of NaCl-salinity on germination, growth rate, and pigment content and raised the osmolytes proline and glycine betaine levels. On the molecular level, two low-molecular-weight proteins were newly synthesized by the NaCl treatments and three by priming pea seeds with SWEs. The number of inter-simple sequence repeats (ISSR) markers increased from 20 in the control to 36 in 150 mM NaCl-treated seedlings, including four unique markers. Priming with SWEs triggered more markers than the control, however about ten of the salinity-induced markers were not detected following seed priming before NaCl treatments. By priming with SWEs, seven unique markers were elicited. Conclusion All in all, priming with SWEs alleviated salinity stress on pea seedlings. Salinity-responsive proteins and ISSR markers are produced in response to salt stress and priming with SWEs.https://doi.org/10.1186/s43141-023-00483-zSeaweedsPeaSalinityOsmoregulationISSR fingerprinting |
| spellingShingle | Marwa M. Hamouda Abdelfattah Badr Sameh S. Ali Alia M. Adham Hanan I. Sayed Ahmed Khalil M. Saad-Allah Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings Journal of Genetic Engineering and Biotechnology Seaweeds Pea Salinity Osmoregulation ISSR fingerprinting |
| title | Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings |
| title_full | Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings |
| title_fullStr | Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings |
| title_full_unstemmed | Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings |
| title_short | Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings |
| title_sort | growth physiological and molecular responses of three phaeophyte extracts on salt stressed pea pisum sativum l seedlings |
| topic | Seaweeds Pea Salinity Osmoregulation ISSR fingerprinting |
| url | https://doi.org/10.1186/s43141-023-00483-z |
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