Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications
Context: In Cuba, the venom of the endemic scorpion Rhopalurus junceus has been used in traditional medicine for cancer treatment. The genetic variability in populations from distinct origin and differences in the composition of venom –a little explored topic- could influence the pharmacological eff...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2021-01-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol9/jppres20.896_9.1.1.pdf |
| _version_ | 1818765374833819648 |
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| author | Rodolfo Rodríguez-Ravelo Ariel Ruíz-Urquiola Lourival D. Possani-Postay Humberto J. Morris-Quevedo Magdelaine Rodríguez-Ravelo Georgina Espinosa-López |
| author_facet | Rodolfo Rodríguez-Ravelo Ariel Ruíz-Urquiola Lourival D. Possani-Postay Humberto J. Morris-Quevedo Magdelaine Rodríguez-Ravelo Georgina Espinosa-López |
| author_sort | Rodolfo Rodríguez-Ravelo |
| collection | DOAJ |
| description | Context: In Cuba, the venom of the endemic scorpion Rhopalurus junceus has been used in traditional medicine for cancer treatment. The genetic variability in populations from distinct origin and differences in the composition of venom –a little explored topic- could influence the pharmacological effects of products used in medical practice.
Aims: To characterize the populations of R. junceus from six different districts of the Moanicum sector in northeastern Cuba, taking into account the variations in the sequences of cytochrome oxidase subunit I gene (COI) and peptide masses of venom.
Methods: For the genetic characterization, a 658 bp gene fragment of COI was amplified by PCR. The genetic structure of populations was analyzed hierarchically, using an analysis of molecular variance (AMOVA). In proteomic analysis, molecular masses of venom constituents were arranged in increasing order, and different components were considered when masses differed by 2 Da.
Results: R. junceus species in the six districts studied in the Moanicum sector belong to different populations. The sequences of COI gene showed high levels of genetic variability, recovering 47 haplotypes (87% unique). The composition of the venom was also significantly different between the districts. The most abundant components presented masses of 3-6 kDa (K+-channel specific peptides) and 6,1-10 kDa (Na+-channel specific peptides).
Conclusions: Populations of R. junceus showed high genetic differentiation and a broad intraspecific variation in the venom peptides (3-10 kDa) in six districts of Moanicum sector of Cuba. This richness in scorpion varieties with different toxins profile should be considered for pharmacological applications. |
| first_indexed | 2024-12-18T08:17:05Z |
| format | Article |
| id | doaj.art-039bf62e0e944f9491d621b194c47f93 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-18T08:17:05Z |
| publishDate | 2021-01-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-039bf62e0e944f9491d621b194c47f932022-12-21T21:14:48ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502021-01-0191112Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implicationsRodolfo Rodríguez-Ravelo0Ariel Ruíz-Urquiola1Lourival D. Possani-Postay2Humberto J. Morris-Quevedo3Magdelaine Rodríguez-Ravelo4Georgina Espinosa-López5Center for Mountain Development, Ministry of Science, Technology and Environment, El Salvador, Guantánamo, Cuba.Museum für Naturkunde, Leibniz Institute for Research on Evolution and Biodiversity, Humboldt University, 10115 Berlin, Germany.Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca 62210, Mexico.Center of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Santiago de Cuba 5, CP 90500, Cuba.Department of Pediatry, Pediatric Hospital “Pedro A. Pérez”, Guantánamo, Cuba.Department of Biochemistry, Faculty of Biology, University of Havana, 25 Street # 455, Vedado, Havana 4, CP 10400, Cuba.Context: In Cuba, the venom of the endemic scorpion Rhopalurus junceus has been used in traditional medicine for cancer treatment. The genetic variability in populations from distinct origin and differences in the composition of venom –a little explored topic- could influence the pharmacological effects of products used in medical practice. Aims: To characterize the populations of R. junceus from six different districts of the Moanicum sector in northeastern Cuba, taking into account the variations in the sequences of cytochrome oxidase subunit I gene (COI) and peptide masses of venom. Methods: For the genetic characterization, a 658 bp gene fragment of COI was amplified by PCR. The genetic structure of populations was analyzed hierarchically, using an analysis of molecular variance (AMOVA). In proteomic analysis, molecular masses of venom constituents were arranged in increasing order, and different components were considered when masses differed by 2 Da. Results: R. junceus species in the six districts studied in the Moanicum sector belong to different populations. The sequences of COI gene showed high levels of genetic variability, recovering 47 haplotypes (87% unique). The composition of the venom was also significantly different between the districts. The most abundant components presented masses of 3-6 kDa (K+-channel specific peptides) and 6,1-10 kDa (Na+-channel specific peptides). Conclusions: Populations of R. junceus showed high genetic differentiation and a broad intraspecific variation in the venom peptides (3-10 kDa) in six districts of Moanicum sector of Cuba. This richness in scorpion varieties with different toxins profile should be considered for pharmacological applications.http://jppres.com/jppres/pdf/vol9/jppres20.896_9.1.1.pdfcytochrome oxidase subunit imolecular variancerhopalurus junceusscorpion venom |
| spellingShingle | Rodolfo Rodríguez-Ravelo Ariel Ruíz-Urquiola Lourival D. Possani-Postay Humberto J. Morris-Quevedo Magdelaine Rodríguez-Ravelo Georgina Espinosa-López Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications Journal of Pharmacy & Pharmacognosy Research cytochrome oxidase subunit i molecular variance rhopalurus junceus scorpion venom |
| title | Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications |
| title_full | Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications |
| title_fullStr | Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications |
| title_full_unstemmed | Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications |
| title_short | Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications |
| title_sort | variation of cytochrome oxidase i gene and venom proteins of rhopalurus junceus populations in the moanicum sector pharmacological implications |
| topic | cytochrome oxidase subunit i molecular variance rhopalurus junceus scorpion venom |
| url | http://jppres.com/jppres/pdf/vol9/jppres20.896_9.1.1.pdf |
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