Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.)
Auxin response factors (ARFs) are transcription factors that regulate the expression of auxin response genes, and have important functions in plant growth and development. In this study, available genome data for peanut (Arachis hypogaea L.) were used to identify AhARF genes. In total, 61 AhARFs and...
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PeerJ Inc.
2021-10-01
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| author | Peipei Li Qian Ma Chengxin Qu Shuliang Zhu Kunkun Zhao Xingli Ma Zhongfeng Li Xingguo Zhang Fangping Gong Dongmei Yin |
| author_facet | Peipei Li Qian Ma Chengxin Qu Shuliang Zhu Kunkun Zhao Xingli Ma Zhongfeng Li Xingguo Zhang Fangping Gong Dongmei Yin |
| author_sort | Peipei Li |
| collection | DOAJ |
| description | Auxin response factors (ARFs) are transcription factors that regulate the expression of auxin response genes, and have important functions in plant growth and development. In this study, available genome data for peanut (Arachis hypogaea L.) were used to identify AhARF genes. In total, 61 AhARFs and 23 AtARFs were divided into six groups (I–VI). Molecular structural analysis revealed that the protein members of AhARF contain at least two domains, the B3 domain and the Auxin-resp domain, and that some have a C-terminal dimerisation domain. Screening of the transcriptome data of 22 tissues of A. hypogaea cv. Tifrunner in a public database showed high expression levels of AhARF2 and AhARF6. AhARF6 was expressed more highly in the stem and branch than in the root and leaf of the wild species Arachis monticola (A. mon) and cultivated species H103. After treatment with exogenous auxin (NAA), the expression of AhARF6 was inhibited, and this inhibition was greater in A. mon than in H103. The transcriptome map revealed that the expression of AhARF6 was higher in the larger pods of H8107 and ZP06 than in the medium pods of H103 and small pods of A. mon. Moreover, AhARF6-5 was proven to be localised in the nucleus, consistent with the location of AtARF6. These results suggest that AhARF6 may play an important role in pod development in peanut. |
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| language | English |
| last_indexed | 2024-03-09T06:25:29Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-039dd343e6da458eb3b5c7a435ad66ca2023-12-03T11:19:41ZengPeerJ Inc.PeerJ2167-83592021-10-019e1231910.7717/peerj.12319Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.)Peipei LiQian MaChengxin QuShuliang ZhuKunkun ZhaoXingli MaZhongfeng LiXingguo ZhangFangping GongDongmei YinAuxin response factors (ARFs) are transcription factors that regulate the expression of auxin response genes, and have important functions in plant growth and development. In this study, available genome data for peanut (Arachis hypogaea L.) were used to identify AhARF genes. In total, 61 AhARFs and 23 AtARFs were divided into six groups (I–VI). Molecular structural analysis revealed that the protein members of AhARF contain at least two domains, the B3 domain and the Auxin-resp domain, and that some have a C-terminal dimerisation domain. Screening of the transcriptome data of 22 tissues of A. hypogaea cv. Tifrunner in a public database showed high expression levels of AhARF2 and AhARF6. AhARF6 was expressed more highly in the stem and branch than in the root and leaf of the wild species Arachis monticola (A. mon) and cultivated species H103. After treatment with exogenous auxin (NAA), the expression of AhARF6 was inhibited, and this inhibition was greater in A. mon than in H103. The transcriptome map revealed that the expression of AhARF6 was higher in the larger pods of H8107 and ZP06 than in the medium pods of H103 and small pods of A. mon. Moreover, AhARF6-5 was proven to be localised in the nucleus, consistent with the location of AtARF6. These results suggest that AhARF6 may play an important role in pod development in peanut.https://peerj.com/articles/12319.pdfPod developmentAuxin response factorsAuxinEvolutionary analysisHormonal response |
| spellingShingle | Peipei Li Qian Ma Chengxin Qu Shuliang Zhu Kunkun Zhao Xingli Ma Zhongfeng Li Xingguo Zhang Fangping Gong Dongmei Yin Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) PeerJ Pod development Auxin response factors Auxin Evolutionary analysis Hormonal response |
| title | Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) |
| title_full | Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) |
| title_fullStr | Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) |
| title_full_unstemmed | Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) |
| title_short | Genome-wide identification and expression analysis of auxin response factors in peanut (Arachis hypogaea L.) |
| title_sort | genome wide identification and expression analysis of auxin response factors in peanut arachis hypogaea l |
| topic | Pod development Auxin response factors Auxin Evolutionary analysis Hormonal response |
| url | https://peerj.com/articles/12319.pdf |
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