Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein
Phospholipid transfer protein (PLTP) transfers phospholipids between HDL and other lipoproteins in plasma. It also remodels spherical, apolipoprotein A-I (apoA-I)-containing HDL into large and small particles in a process involving the dissociation of lipid-free/lipid-poor apoA-I. ApoE is another ap...
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| Format: | Article |
| Language: | English |
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Elsevier
2008-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752042886X |
| _version_ | 1818639756297240576 |
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| author | Nongnuch Settasatian Philip J. Barter Kerry-Anne Rye |
| author_facet | Nongnuch Settasatian Philip J. Barter Kerry-Anne Rye |
| author_sort | Nongnuch Settasatian |
| collection | DOAJ |
| description | Phospholipid transfer protein (PLTP) transfers phospholipids between HDL and other lipoproteins in plasma. It also remodels spherical, apolipoprotein A-I (apoA-I)-containing HDL into large and small particles in a process involving the dissociation of lipid-free/lipid-poor apoA-I. ApoE is another apolipoprotein that is mostly associated with large, spherical HDL that do not contain apoA-I. Three isoforms of apoE have been identified in human plasma: apoE2, apoE3, and apoE4. This study investigates the remodeling of spherical apoE-containing HDL by PLTP and the ability of PLTP to transfer phospholipids between apoE-containing HDL and phospholipid vesicles. Spherical reconstituted high density lipoproteins (rHDL) containing apoA-I [(A-I)rHDL], apoE2 [(E2)rHDL], apoE3 [(E3)rHDL], or apoE4 [(E4)rHDL] as the sole apolipoprotein were prepared by incubating discoidal rHDL with low density lipoproteins and lecithin:cholesterol acyltransferase. PLTP remodeled the spherical, apoE-containing rHDL into large and small particles without the dissociation of apoE. The PLTP-mediated remodeling of apoE-containing rHDL was more extensive than that of (A-I)rHDL. PLTP transferred phospholipids from small unilamellar vesicles to apoE-containing rHDL in an isoform-dependent manner, but at a rate slower than that for spherical (A-I)rHDL. It is concluded that apoE enhances the capacity of PLTP to remodel HDL but reduces the ability of HDL to participate in PLTP-mediated phospholipid transfers. |
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| format | Article |
| id | doaj.art-039ea6f765524a53b1d05d7b366a4f32 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T23:00:26Z |
| publishDate | 2008-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-039ea6f765524a53b1d05d7b366a4f322022-12-21T22:12:43ZengElsevierJournal of Lipid Research0022-22752008-01-01491115126Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer proteinNongnuch Settasatian0Philip J. Barter1Kerry-Anne Rye2Lipid Research Group, Heart Research Institute, Sydney, New South Wales 2050, AustraliaLipid Research Group, Heart Research Institute, Sydney, New South Wales 2050, Australia; Faculty of Medicine, University of Sydney, Sydney, New South Wales 2006, AustraliaLipid Research Group, Heart Research Institute, Sydney, New South Wales 2050, Australia; Faculty of Medicine, University of Sydney, Sydney, New South Wales 2006, Australia; Department of Medicine, University of Melbourne, Melbourne, Victoria 3010, AustraliaPhospholipid transfer protein (PLTP) transfers phospholipids between HDL and other lipoproteins in plasma. It also remodels spherical, apolipoprotein A-I (apoA-I)-containing HDL into large and small particles in a process involving the dissociation of lipid-free/lipid-poor apoA-I. ApoE is another apolipoprotein that is mostly associated with large, spherical HDL that do not contain apoA-I. Three isoforms of apoE have been identified in human plasma: apoE2, apoE3, and apoE4. This study investigates the remodeling of spherical apoE-containing HDL by PLTP and the ability of PLTP to transfer phospholipids between apoE-containing HDL and phospholipid vesicles. Spherical reconstituted high density lipoproteins (rHDL) containing apoA-I [(A-I)rHDL], apoE2 [(E2)rHDL], apoE3 [(E3)rHDL], or apoE4 [(E4)rHDL] as the sole apolipoprotein were prepared by incubating discoidal rHDL with low density lipoproteins and lecithin:cholesterol acyltransferase. PLTP remodeled the spherical, apoE-containing rHDL into large and small particles without the dissociation of apoE. The PLTP-mediated remodeling of apoE-containing rHDL was more extensive than that of (A-I)rHDL. PLTP transferred phospholipids from small unilamellar vesicles to apoE-containing rHDL in an isoform-dependent manner, but at a rate slower than that for spherical (A-I)rHDL. It is concluded that apoE enhances the capacity of PLTP to remodel HDL but reduces the ability of HDL to participate in PLTP-mediated phospholipid transfers.http://www.sciencedirect.com/science/article/pii/S002222752042886Xhigh density lipoprotein fusionphospholipid transfersHDL metabolism |
| spellingShingle | Nongnuch Settasatian Philip J. Barter Kerry-Anne Rye Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein Journal of Lipid Research high density lipoprotein fusion phospholipid transfers HDL metabolism |
| title | Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| title_full | Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| title_fullStr | Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| title_full_unstemmed | Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| title_short | Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| title_sort | remodeling of apolipoprotein e containing spherical reconstituted high density lipoproteins by phospholipid transfer protein |
| topic | high density lipoprotein fusion phospholipid transfers HDL metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S002222752042886X |
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