Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico
The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP)....
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Sociedade Brasileira de Genética
2008-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013 |
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| author | Ilda G. Fernández José Gonzalo Ríos Ramírez Amanda Gayosso Vázquez Raúl Ulloa Arvizu Rogelio A. Alonso Morales |
| author_facet | Ilda G. Fernández José Gonzalo Ríos Ramírez Amanda Gayosso Vázquez Raúl Ulloa Arvizu Rogelio A. Alonso Morales |
| author_sort | Ilda G. Fernández |
| collection | DOAJ |
| description | The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua F IS = 0.1 (p = 0.019) and Tamaulipas F IS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations. |
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| id | doaj.art-039f609c8dcd45bba3760fadc07ce4dc |
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| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-13T04:03:39Z |
| publishDate | 2008-01-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-039f609c8dcd45bba3760fadc07ce4dc2022-12-22T00:00:19ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-01314880886Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern MexicoIlda G. FernándezJosé Gonzalo Ríos RamírezAmanda Gayosso VázquezRaúl Ulloa ArvizuRogelio A. Alonso MoralesThe polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua F IS = 0.1 (p = 0.019) and Tamaulipas F IS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013BoLA-DRB3.2polymorphismCreole cattlePCR-RFLP |
| spellingShingle | Ilda G. Fernández José Gonzalo Ríos Ramírez Amanda Gayosso Vázquez Raúl Ulloa Arvizu Rogelio A. Alonso Morales Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico Genetics and Molecular Biology BoLA-DRB3.2 polymorphism Creole cattle PCR-RFLP |
| title | Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico |
| title_full | Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico |
| title_fullStr | Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico |
| title_full_unstemmed | Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico |
| title_short | Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico |
| title_sort | polymorphism of locus drb3 2 in populations of creole cattle from northern mexico |
| topic | BoLA-DRB3.2 polymorphism Creole cattle PCR-RFLP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013 |
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