E-cadherin promotes incorporation of mouse epiblast stem cells into normal development.
Mouse epiblast stem cells (mEpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct from that of mouse embryonic stem cells (mESCs) in several cell biological criteria. One of the distinctions is that mEpiSCs contribute either not a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3445497?pdf=render |
| _version_ | 1818945923296788480 |
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| author | Satoshi Ohtsuka Satomi Nishikawa-Torikai Hitoshi Niwa |
| author_facet | Satoshi Ohtsuka Satomi Nishikawa-Torikai Hitoshi Niwa |
| author_sort | Satoshi Ohtsuka |
| collection | DOAJ |
| description | Mouse epiblast stem cells (mEpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct from that of mouse embryonic stem cells (mESCs) in several cell biological criteria. One of the distinctions is that mEpiSCs contribute either not at all or at much lower efficiency to chimeric embryos after blastocyst injection compared to mESCs. However, here we showed that mEpiSCs can be incorporated into normal development after blastocyst injection by forced expression of the E-cadherin transgene for 2 days in culture. Using this strategy, mEpiSCs gave rise to live-born chimeras from 5% of the manipulated blastocysts. There were no obvious signs of reprogramming of mEpiSCs toward the mESC-like state during the 2 days after induction of the E-cadherin transgene, suggesting that mEpiSCs possess latent ability to integrate into the normal developmental process as its origin, epiblasts. |
| first_indexed | 2024-12-20T08:06:49Z |
| format | Article |
| id | doaj.art-03a0aeb40b5d4df9bf57fce5f95b0a28 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:06:49Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-03a0aeb40b5d4df9bf57fce5f95b0a282022-12-21T19:47:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4522010.1371/journal.pone.0045220E-cadherin promotes incorporation of mouse epiblast stem cells into normal development.Satoshi OhtsukaSatomi Nishikawa-TorikaiHitoshi NiwaMouse epiblast stem cells (mEpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct from that of mouse embryonic stem cells (mESCs) in several cell biological criteria. One of the distinctions is that mEpiSCs contribute either not at all or at much lower efficiency to chimeric embryos after blastocyst injection compared to mESCs. However, here we showed that mEpiSCs can be incorporated into normal development after blastocyst injection by forced expression of the E-cadherin transgene for 2 days in culture. Using this strategy, mEpiSCs gave rise to live-born chimeras from 5% of the manipulated blastocysts. There were no obvious signs of reprogramming of mEpiSCs toward the mESC-like state during the 2 days after induction of the E-cadherin transgene, suggesting that mEpiSCs possess latent ability to integrate into the normal developmental process as its origin, epiblasts.http://europepmc.org/articles/PMC3445497?pdf=render |
| spellingShingle | Satoshi Ohtsuka Satomi Nishikawa-Torikai Hitoshi Niwa E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. PLoS ONE |
| title | E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. |
| title_full | E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. |
| title_fullStr | E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. |
| title_full_unstemmed | E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. |
| title_short | E-cadherin promotes incorporation of mouse epiblast stem cells into normal development. |
| title_sort | e cadherin promotes incorporation of mouse epiblast stem cells into normal development |
| url | http://europepmc.org/articles/PMC3445497?pdf=render |
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