Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes
<i>Curcuma mangga</i> Val. & Zijp. is a member of the Zingiberaceae family commonly grown in Thailand. It is locally known as mango tumeric because of its mango-like smell when the fresh rhizomes are cut. C. mangga is a popular vegetable, the tips of the young rhizomes and shoots are...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2009-08-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-3/0125-3395-31-3-293-297.pdf |
| _version_ | 1811323064782159872 |
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| author | Kanidta Kaewkroek Chatchai Wattanapiromsakul Supinya Tewtrakul |
| author_facet | Kanidta Kaewkroek Chatchai Wattanapiromsakul Supinya Tewtrakul |
| author_sort | Kanidta Kaewkroek |
| collection | DOAJ |
| description | <i>Curcuma mangga</i> Val. & Zijp. is a member of the Zingiberaceae family commonly grown in Thailand. It is locally known as mango tumeric because of its mango-like smell when the fresh rhizomes are cut. C. mangga is a popular vegetable, the tips of the young rhizomes and shoots are consumed raw with rice. Medicinally, the rhizomes are used as a stomachic and for chest pains, fever, and general debility. It is also used in postpartum care. In the present study, we investigated the anti-inflammatory effect of the extract and compounds from C. mangga rhizomes against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cell line. From bioassay-guided fractionation, the chloroform fraction exhibited the most potent inhibitory activity with an IC50 value of 2.1 g/ml, followed by the hexane fraction (IC50 = 3.8 g/ml) and the ethyl acetate fraction (IC50 = 23.5 g/ml), respectively. Demethoxycurcumin (1) and 3-buten-2-one, 4-[(1R, 4aR, 8aR)-decahydro-5, 5, 8a-trimethyl-2-methylene-1-naphthalenyl]-, (3E)-rel- (2) were isolated from the chloroform- and hexane fractions, respectively. Bisdemethoxycurcumin (3) whose structure is similar to that of 1 was also tested for NO inhibitory activity. Of the tested compounds, compound 1 exhibited the highest activity with an IC50 value of 12.1 μM, followed by 3(IC50 = 16.9 M) and 2 (IC50 = 30.3 M). These results suggest that C. mangga and its compounds exert NO inhibitory activity and have a potential to be developed as a pharmaceutical preparation for treatment of inflammatory-related diseases. Moreover, this is the first report of compound 2 that was isolated from C. mangga rhizomes. |
| first_indexed | 2024-04-13T13:47:24Z |
| format | Article |
| id | doaj.art-03a42e8a36b64412a0b37e45911b7f84 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-13T13:47:24Z |
| publishDate | 2009-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-03a42e8a36b64412a0b37e45911b7f842022-12-22T02:44:26ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952009-08-01313293297Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomesKanidta KaewkroekChatchai WattanapiromsakulSupinya Tewtrakul<i>Curcuma mangga</i> Val. & Zijp. is a member of the Zingiberaceae family commonly grown in Thailand. It is locally known as mango tumeric because of its mango-like smell when the fresh rhizomes are cut. C. mangga is a popular vegetable, the tips of the young rhizomes and shoots are consumed raw with rice. Medicinally, the rhizomes are used as a stomachic and for chest pains, fever, and general debility. It is also used in postpartum care. In the present study, we investigated the anti-inflammatory effect of the extract and compounds from C. mangga rhizomes against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cell line. From bioassay-guided fractionation, the chloroform fraction exhibited the most potent inhibitory activity with an IC50 value of 2.1 g/ml, followed by the hexane fraction (IC50 = 3.8 g/ml) and the ethyl acetate fraction (IC50 = 23.5 g/ml), respectively. Demethoxycurcumin (1) and 3-buten-2-one, 4-[(1R, 4aR, 8aR)-decahydro-5, 5, 8a-trimethyl-2-methylene-1-naphthalenyl]-, (3E)-rel- (2) were isolated from the chloroform- and hexane fractions, respectively. Bisdemethoxycurcumin (3) whose structure is similar to that of 1 was also tested for NO inhibitory activity. Of the tested compounds, compound 1 exhibited the highest activity with an IC50 value of 12.1 μM, followed by 3(IC50 = 16.9 M) and 2 (IC50 = 30.3 M). These results suggest that C. mangga and its compounds exert NO inhibitory activity and have a potential to be developed as a pharmaceutical preparation for treatment of inflammatory-related diseases. Moreover, this is the first report of compound 2 that was isolated from C. mangga rhizomes.http://www.rdoapp.psu.ac.th/html/sjst/journal/31-3/0125-3395-31-3-293-297.pdfRAW264.7 cellnitric oxide<i>Curcuma mangga</i>Zingiberaceae |
| spellingShingle | Kanidta Kaewkroek Chatchai Wattanapiromsakul Supinya Tewtrakul Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes Songklanakarin Journal of Science and Technology (SJST) RAW264.7 cell nitric oxide <i>Curcuma mangga</i> Zingiberaceae |
| title | Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes |
| title_full | Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes |
| title_fullStr | Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes |
| title_full_unstemmed | Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes |
| title_short | Nitric oxide inhibitory substances from <i>Curcuma mangga</i> rhizomes |
| title_sort | nitric oxide inhibitory substances from i curcuma mangga i rhizomes |
| topic | RAW264.7 cell nitric oxide <i>Curcuma mangga</i> Zingiberaceae |
| url | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-3/0125-3395-31-3-293-297.pdf |
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