Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran
Background: The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. Ribosome-Inactivating Proteins (RIP...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shaheed Beheshti University of Medical Sciences
2019-10-01
|
| Series: | International Journal of Medical Toxicology and Forensic Medicine |
| Subjects: | |
| Online Access: | https://journals.sbmu.ac.ir/ijmtfm/article/view/25706/pdf |
| _version_ | 1828784503777132544 |
|---|---|
| author | Mohammad Hosseinpour Jamil Zargan Hossein Honari Ashkan Haji Nour Mohammadi Abbas Hajizadeh Hani Keshavarz Alikhani Ahmad Heidari Ehsan Zamani |
| author_facet | Mohammad Hosseinpour Jamil Zargan Hossein Honari Ashkan Haji Nour Mohammadi Abbas Hajizadeh Hani Keshavarz Alikhani Ahmad Heidari Ehsan Zamani |
| author_sort | Mohammad Hosseinpour |
| collection | DOAJ |
| description | Background: The production and secretion of defense proteins are one of the protective
mechanisms exploited by plants against pathogens. The production and secretion of defense
proteins are one of the protective mechanisms exploited by plants against pathogens.
Ribosome-Inactivating Proteins (RIPs), as the main class of these proteins, are considered
to facilitate cancer therapy worldwide, because of the potential anticancer activity. Indeed,
some of these proteins have cytotoxic and anticancer properties. Extracted from the carnation
(Dianthus caryophyllus), Dianthin inhibits protein synthesis in many different cells.
Methods: In this research, the Dianthins was isolated and purified from the leaves of
D. caryophyllus, using ion-exchange chromatography column (CM-Sephadex G-50).
Subsequently, its cytotoxicity effect on MCF-7 cell line was investigated. The cell cytotoxicity
assessment was performed, using 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium
bromide (MTT), neutral red uptake, and alkaline comet assays at the concentrations of 1.25μg/
mL to 10μg/mL of the protein applying the MCF-7 cell line.
Results: the toxin induces cell death, mostly via necrosis rather than apoptosis, but in the
special range of concentrations.
Conclusion: because of the severe side effects of chemotherapy drugs, this toxin can undergo
more research as a new drug candidate against breast cancer |
| first_indexed | 2024-12-11T23:32:40Z |
| format | Article |
| id | doaj.art-03a4f49e70144253a10e8881a164d338 |
| institution | Directory Open Access Journal |
| issn | 2251-8762 2251-8770 |
| language | English |
| last_indexed | 2024-12-11T23:32:40Z |
| publishDate | 2019-10-01 |
| publisher | Shaheed Beheshti University of Medical Sciences |
| record_format | Article |
| series | International Journal of Medical Toxicology and Forensic Medicine |
| spelling | doaj.art-03a4f49e70144253a10e8881a164d3382022-12-22T00:45:58ZengShaheed Beheshti University of Medical SciencesInternational Journal of Medical Toxicology and Forensic Medicine2251-87622251-87702019-10-0193133.14010.32598/ijmtfm.v9i3.25706Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in IranMohammad Hosseinpour0https://orcid.org/0000-0002-4614-8794Jamil Zargan1https://orcid.org/0000-0002-1655-4265Hossein Honari2Ashkan Haji Nour Mohammadi3Abbas Hajizadeh4Hani Keshavarz Alikhani5Ahmad Heidari6Ehsan Zamani7Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran.Department of Biology, Faculty of Basic Sciences, Zanjan University, Zanjan, Iran.Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.Background: The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. Ribosome-Inactivating Proteins (RIPs), as the main class of these proteins, are considered to facilitate cancer therapy worldwide, because of the potential anticancer activity. Indeed, some of these proteins have cytotoxic and anticancer properties. Extracted from the carnation (Dianthus caryophyllus), Dianthin inhibits protein synthesis in many different cells. Methods: In this research, the Dianthins was isolated and purified from the leaves of D. caryophyllus, using ion-exchange chromatography column (CM-Sephadex G-50). Subsequently, its cytotoxicity effect on MCF-7 cell line was investigated. The cell cytotoxicity assessment was performed, using 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), neutral red uptake, and alkaline comet assays at the concentrations of 1.25μg/ mL to 10μg/mL of the protein applying the MCF-7 cell line. Results: the toxin induces cell death, mostly via necrosis rather than apoptosis, but in the special range of concentrations. Conclusion: because of the severe side effects of chemotherapy drugs, this toxin can undergo more research as a new drug candidate against breast cancerhttps://journals.sbmu.ac.ir/ijmtfm/article/view/25706/pdfdianthindianthus caryophyllusmtt assayneutral red uptake assaymcf-7 |
| spellingShingle | Mohammad Hosseinpour Jamil Zargan Hossein Honari Ashkan Haji Nour Mohammadi Abbas Hajizadeh Hani Keshavarz Alikhani Ahmad Heidari Ehsan Zamani Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran International Journal of Medical Toxicology and Forensic Medicine dianthin dianthus caryophyllus mtt assay neutral red uptake assay mcf-7 |
| title | Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran |
| title_full | Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran |
| title_fullStr | Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran |
| title_full_unstemmed | Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran |
| title_short | Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran |
| title_sort | introduction of dianthins a new promising horizon toward continuous research on breast cancer bulldozing in iran |
| topic | dianthin dianthus caryophyllus mtt assay neutral red uptake assay mcf-7 |
| url | https://journals.sbmu.ac.ir/ijmtfm/article/view/25706/pdf |
| work_keys_str_mv | AT mohammadhosseinpour introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT jamilzargan introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT hosseinhonari introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT ashkanhajinourmohammadi introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT abbashajizadeh introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT hanikeshavarzalikhani introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT ahmadheidari introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran AT ehsanzamani introductionofdianthinsanewpromisinghorizontowardcontinuousresearchonbreastcancerbulldozinginiran |