Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension
Abstract Background The role of interventricular mechanics in pediatric pulmonary arterial hypertension (PAH) and its relation to right ventricular (RV) dysfunction has been largely overlooked. Here, we characterize the impact of maintained pressure overload in the RV–pulmonary artery (PA) axis on m...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of Cardiovascular Magnetic Resonance |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12968-021-00759-8 |
| _version_ | 1797204368380919808 |
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| author | Melanie J. Dufva Mario Boehm Kenzo Ichimura Uyen Truong Xulei Qin Jennifer Tabakh Kendall S. Hunter Dunbar Ivy Edda Spiekerkoetter Vitaly O. Kheyfets |
| author_facet | Melanie J. Dufva Mario Boehm Kenzo Ichimura Uyen Truong Xulei Qin Jennifer Tabakh Kendall S. Hunter Dunbar Ivy Edda Spiekerkoetter Vitaly O. Kheyfets |
| author_sort | Melanie J. Dufva |
| collection | DOAJ |
| description | Abstract Background The role of interventricular mechanics in pediatric pulmonary arterial hypertension (PAH) and its relation to right ventricular (RV) dysfunction has been largely overlooked. Here, we characterize the impact of maintained pressure overload in the RV–pulmonary artery (PA) axis on myocardial strain and left ventricular (LV) mechanics in pediatric PAH patients in comparison to a preclinical PA-banding (PAB) mouse model. We hypothesize that the PAB mouse model mimics important aspects of interventricular mechanics of pediatric PAH and may be beneficial as a surrogate model for some longitudinal and interventional studies not possible in children. Methods Balanced steady-state free precession (bSSFP) cardiovascular magnetic resonance (CMR) images of 18 PAH and 17 healthy (control) pediatric subjects were retrospectively analyzed using CMR feature-tracking (FT) software to compute measurements of myocardial strain. Furthermore, myocardial tagged-CMR images were also analyzed for each subject using harmonic phase flow analysis to derive LV torsion rate. Within 48 h of CMR, PAH patients underwent right heart catheterization (RHC) for measurement of PA/RV pressures, and to compute RV end-systolic elastance (RV_Ees, a measure of load-independent contractility). Surgical PAB was performed on mice to induce RV pressure overload and myocardial remodeling. bSSFP-CMR, tagged CMR, and intra-cardiac catheterization were performed on 12 PAB and 9 control mice (Sham) 7 weeks after surgery with identical post-processing as in the aforementioned patient studies. RV_Ees was assessed via the single beat method. Results LV torsion rate was significantly reduced under hypertensive conditions in both PAB mice (p = 0.004) and pediatric PAH patients (p < 0.001). This decrease in LV torsion rate correlated significantly with a decrease in RV_Ees in PAB (r = 0.91, p = 0.05) and PAH subjects (r = 0.51, p = 0.04). In order to compare combined metrics of LV torsion rate and strain parameters principal component analysis (PCA) was used. PCA revealed grouping of PAH patients with PAB mice and control subjects with Sham mice. Similar to LV torsion rate, LV global peak circumferential, radial, and longitudinal strain were significantly (p < 0.05) reduced under hypertensive conditions in both PAB mice and children with PAH. Conclusions The PAB mouse model resembles PAH-associated myocardial mechanics and may provide a potential model to study mechanisms of RV/LV interdependency. |
| first_indexed | 2024-04-24T08:34:07Z |
| format | Article |
| id | doaj.art-03a5063cd8724511a0bdf3116bd5b1f8 |
| institution | Directory Open Access Journal |
| issn | 1532-429X |
| language | English |
| last_indexed | 2024-04-24T08:34:07Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Cardiovascular Magnetic Resonance |
| spelling | doaj.art-03a5063cd8724511a0bdf3116bd5b1f82024-04-16T17:38:15ZengElsevierJournal of Cardiovascular Magnetic Resonance1532-429X2021-06-0123111410.1186/s12968-021-00759-8Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertensionMelanie J. Dufva0Mario Boehm1Kenzo Ichimura2Uyen Truong3Xulei Qin4Jennifer Tabakh5Kendall S. Hunter6Dunbar Ivy7Edda Spiekerkoetter8Vitaly O. Kheyfets9Department of Bioengineering, University of Colorado DenverUniversities of Giessen and Marburg Lung Center (UGMLC), Justus-Liebig University Giessen, German Center for Lung Research (DZL)Department of Medicine, Division of Pulmonary and Critical Care Medicine, Stanford UniversityDepartment of Pediatrics, Section of Cardiology, Childrens Hospital ColoradoCardiovascular Institute, Stanford UniversityDepartment of Bioengineering, University of Colorado DenverDepartment of Bioengineering, University of Colorado DenverDepartment of Pediatrics, Section of Cardiology, Childrens Hospital ColoradoDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, Stanford UniversityDepartment of Bioengineering, University of Colorado DenverAbstract Background The role of interventricular mechanics in pediatric pulmonary arterial hypertension (PAH) and its relation to right ventricular (RV) dysfunction has been largely overlooked. Here, we characterize the impact of maintained pressure overload in the RV–pulmonary artery (PA) axis on myocardial strain and left ventricular (LV) mechanics in pediatric PAH patients in comparison to a preclinical PA-banding (PAB) mouse model. We hypothesize that the PAB mouse model mimics important aspects of interventricular mechanics of pediatric PAH and may be beneficial as a surrogate model for some longitudinal and interventional studies not possible in children. Methods Balanced steady-state free precession (bSSFP) cardiovascular magnetic resonance (CMR) images of 18 PAH and 17 healthy (control) pediatric subjects were retrospectively analyzed using CMR feature-tracking (FT) software to compute measurements of myocardial strain. Furthermore, myocardial tagged-CMR images were also analyzed for each subject using harmonic phase flow analysis to derive LV torsion rate. Within 48 h of CMR, PAH patients underwent right heart catheterization (RHC) for measurement of PA/RV pressures, and to compute RV end-systolic elastance (RV_Ees, a measure of load-independent contractility). Surgical PAB was performed on mice to induce RV pressure overload and myocardial remodeling. bSSFP-CMR, tagged CMR, and intra-cardiac catheterization were performed on 12 PAB and 9 control mice (Sham) 7 weeks after surgery with identical post-processing as in the aforementioned patient studies. RV_Ees was assessed via the single beat method. Results LV torsion rate was significantly reduced under hypertensive conditions in both PAB mice (p = 0.004) and pediatric PAH patients (p < 0.001). This decrease in LV torsion rate correlated significantly with a decrease in RV_Ees in PAB (r = 0.91, p = 0.05) and PAH subjects (r = 0.51, p = 0.04). In order to compare combined metrics of LV torsion rate and strain parameters principal component analysis (PCA) was used. PCA revealed grouping of PAH patients with PAB mice and control subjects with Sham mice. Similar to LV torsion rate, LV global peak circumferential, radial, and longitudinal strain were significantly (p < 0.05) reduced under hypertensive conditions in both PAB mice and children with PAH. Conclusions The PAB mouse model resembles PAH-associated myocardial mechanics and may provide a potential model to study mechanisms of RV/LV interdependency.https://doi.org/10.1186/s12968-021-00759-8Pulmonary arterial hypertensionPediatricsVentricular mechanicsFT-CMRTagged-CMRCardiovascular magnetic resonance |
| spellingShingle | Melanie J. Dufva Mario Boehm Kenzo Ichimura Uyen Truong Xulei Qin Jennifer Tabakh Kendall S. Hunter Dunbar Ivy Edda Spiekerkoetter Vitaly O. Kheyfets Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension Journal of Cardiovascular Magnetic Resonance Pulmonary arterial hypertension Pediatrics Ventricular mechanics FT-CMR Tagged-CMR Cardiovascular magnetic resonance |
| title | Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| title_full | Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| title_fullStr | Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| title_full_unstemmed | Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| title_short | Pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| title_sort | pulmonary arterial banding in mice may be a suitable model for studies on ventricular mechanics in pediatric pulmonary arterial hypertension |
| topic | Pulmonary arterial hypertension Pediatrics Ventricular mechanics FT-CMR Tagged-CMR Cardiovascular magnetic resonance |
| url | https://doi.org/10.1186/s12968-021-00759-8 |
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