Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort
The duration of humoral and cellular immune memory following SARS-CoV-2 infection in populations in least developed countries remains understudied but is key to overcome the current SARS-CoV-2 pandemic. Sixty-four Cambodian individuals with laboratory-confirmed infection with asymptomatic or mild/mo...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.817905/full |
| _version_ | 1828142138172375040 |
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| author | Hoa Thi My Vo Alvino Maestri Heidi Auerswald Sopheak Sorn Sokchea Lay Heng Seng Sotheary Sann Nisa Ya Polidy Pean Philippe Dussart Olivier Schwartz Olivier Schwartz Sovann Ly Timothée Bruel Timothée Bruel Sowath Ly Veasna Duong Erik A. Karlsson Tineke Cantaert |
| author_facet | Hoa Thi My Vo Alvino Maestri Heidi Auerswald Sopheak Sorn Sokchea Lay Heng Seng Sotheary Sann Nisa Ya Polidy Pean Philippe Dussart Olivier Schwartz Olivier Schwartz Sovann Ly Timothée Bruel Timothée Bruel Sowath Ly Veasna Duong Erik A. Karlsson Tineke Cantaert |
| author_sort | Hoa Thi My Vo |
| collection | DOAJ |
| description | The duration of humoral and cellular immune memory following SARS-CoV-2 infection in populations in least developed countries remains understudied but is key to overcome the current SARS-CoV-2 pandemic. Sixty-four Cambodian individuals with laboratory-confirmed infection with asymptomatic or mild/moderate clinical presentation were evaluated for Spike (S)-binding and neutralizing antibodies and antibody effector functions during acute phase of infection and at 6-9 months follow-up. Antigen-specific B cells, CD4+ and CD8+ T cells were characterized, and T cells were interrogated for functionality at late convalescence. Anti-S antibody titers decreased over time, but effector functions mediated by S-specific antibodies remained stable. S- and nucleocapsid (N)-specific B cells could be detected in late convalescence in the activated memory B cell compartment and are mostly IgG+. CD4+ and CD8+ T cell immune memory was maintained to S and membrane (M) protein. Asymptomatic infection resulted in decreased antibody-dependent cellular cytotoxicity (ADCC) and frequency of SARS-CoV-2-specific CD4+ T cells at late convalescence. Whereas anti-S antibodies correlated with S-specific B cells, there was no correlation between T cell response and humoral immune memory. Hence, all aspects of a protective immune response are maintained up to nine months after SARS-CoV-2 infection and in the absence of re-infection. |
| first_indexed | 2024-04-11T19:36:54Z |
| format | Article |
| id | doaj.art-03a5e8412618454fbb1fa3cdfb245325 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T19:36:54Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-03a5e8412618454fbb1fa3cdfb2453252022-12-22T04:06:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-02-011310.3389/fimmu.2022.817905817905Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal CohortHoa Thi My Vo0Alvino Maestri1Heidi Auerswald2Sopheak Sorn3Sokchea Lay4Heng Seng5Sotheary Sann6Nisa Ya7Polidy Pean8Philippe Dussart9Olivier Schwartz10Olivier Schwartz11Sovann Ly12Timothée Bruel13Timothée Bruel14Sowath Ly15Veasna Duong16Erik A. Karlsson17Tineke Cantaert18Immunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaVirology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaEpidemiology and Public Health Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, Cambodia Department of Communicable Disease Control, Ministry of Health (CDC-MoH), Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaVirology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaInstitut Pasteur, Université de Paris, CNRS UMR3569, Virus and Immunity Unit, Paris, FranceVaccine Research Institute, Créteil, France Department of Communicable Disease Control, Ministry of Health (CDC-MoH), Phnom Penh, CambodiaInstitut Pasteur, Université de Paris, CNRS UMR3569, Virus and Immunity Unit, Paris, FranceVaccine Research Institute, Créteil, FranceEpidemiology and Public Health Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaVirology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaVirology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaImmunology Unit, Institut Pasteur du Cambodge, Pasteur Network, Phnom Penh, CambodiaThe duration of humoral and cellular immune memory following SARS-CoV-2 infection in populations in least developed countries remains understudied but is key to overcome the current SARS-CoV-2 pandemic. Sixty-four Cambodian individuals with laboratory-confirmed infection with asymptomatic or mild/moderate clinical presentation were evaluated for Spike (S)-binding and neutralizing antibodies and antibody effector functions during acute phase of infection and at 6-9 months follow-up. Antigen-specific B cells, CD4+ and CD8+ T cells were characterized, and T cells were interrogated for functionality at late convalescence. Anti-S antibody titers decreased over time, but effector functions mediated by S-specific antibodies remained stable. S- and nucleocapsid (N)-specific B cells could be detected in late convalescence in the activated memory B cell compartment and are mostly IgG+. CD4+ and CD8+ T cell immune memory was maintained to S and membrane (M) protein. Asymptomatic infection resulted in decreased antibody-dependent cellular cytotoxicity (ADCC) and frequency of SARS-CoV-2-specific CD4+ T cells at late convalescence. Whereas anti-S antibodies correlated with S-specific B cells, there was no correlation between T cell response and humoral immune memory. Hence, all aspects of a protective immune response are maintained up to nine months after SARS-CoV-2 infection and in the absence of re-infection.https://www.frontiersin.org/articles/10.3389/fimmu.2022.817905/fullSARS-CoV-2B cell immunityT cell immunityantibody effector functionlong term immune response |
| spellingShingle | Hoa Thi My Vo Alvino Maestri Heidi Auerswald Sopheak Sorn Sokchea Lay Heng Seng Sotheary Sann Nisa Ya Polidy Pean Philippe Dussart Olivier Schwartz Olivier Schwartz Sovann Ly Timothée Bruel Timothée Bruel Sowath Ly Veasna Duong Erik A. Karlsson Tineke Cantaert Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort Frontiers in Immunology SARS-CoV-2 B cell immunity T cell immunity antibody effector function long term immune response |
| title | Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort |
| title_full | Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort |
| title_fullStr | Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort |
| title_full_unstemmed | Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort |
| title_short | Robust and Functional Immune Memory Up to 9 Months After SARS-CoV-2 Infection: A Southeast Asian Longitudinal Cohort |
| title_sort | robust and functional immune memory up to 9 months after sars cov 2 infection a southeast asian longitudinal cohort |
| topic | SARS-CoV-2 B cell immunity T cell immunity antibody effector function long term immune response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.817905/full |
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