Spontaneous Abortion and Chikungunya Infection: Pathological Findings
Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of preg...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/4/554 |
| _version_ | 1797539931250229248 |
|---|---|
| author | Natália Salomão Michelle Brendolin Kíssila Rabelo Mayumi Wakimoto Ana Maria de Filippis Flavia dos Santos Maria Elizabeth Moreira Carlos Alberto Basílio-de-Oliveira Elyzabeth Avvad-Portari Marciano Paes Patrícia Brasil |
| author_facet | Natália Salomão Michelle Brendolin Kíssila Rabelo Mayumi Wakimoto Ana Maria de Filippis Flavia dos Santos Maria Elizabeth Moreira Carlos Alberto Basílio-de-Oliveira Elyzabeth Avvad-Portari Marciano Paes Patrícia Brasil |
| author_sort | Natália Salomão |
| collection | DOAJ |
| description | Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death. |
| first_indexed | 2024-03-10T12:52:50Z |
| format | Article |
| id | doaj.art-03a6caacc1ad4a8a9c27c95057b1e48e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T12:52:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-03a6caacc1ad4a8a9c27c95057b1e48e2023-11-21T12:05:13ZengMDPI AGViruses1999-49152021-03-0113455410.3390/v13040554Spontaneous Abortion and Chikungunya Infection: Pathological FindingsNatália Salomão0Michelle Brendolin1Kíssila Rabelo2Mayumi Wakimoto3Ana Maria de Filippis4Flavia dos Santos5Maria Elizabeth Moreira6Carlos Alberto Basílio-de-Oliveira7Elyzabeth Avvad-Portari8Marciano Paes9Patrícia Brasil10Interdisciplinary Medical Research Laboratory Rio de Janeiro, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilAcute Febrile Diseases Laboratory, Evandro Chagas National Infectiology Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, BrazilUltrastructure and Tissue Biology Laboratory Rio de Janeiro, Rio de Janeiro State University, Rio de Janeiro 20551-030, BrazilAcute Febrile Diseases Laboratory, Evandro Chagas National Infectiology Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, BrazilFlaviviruses Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilViral Immunology Laboratory, Oswaldo Cruz Institute Rio de Janeiro, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilNational Institute of Women, Children and Adolescents Health Fernandes Figueira, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilPathological Anatomy, Gaffrée Guinle University Hospital Rio de Janeiro, Federal University of the State of Rio de Janeiro, Rio de Janeiro 20270-004, BrazilNational Institute of Women, Children and Adolescents Health Fernandes Figueira, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilInterdisciplinary Medical Research Laboratory Rio de Janeiro, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, BrazilAcute Febrile Diseases Laboratory, Evandro Chagas National Infectiology Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, BrazilIntrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death.https://www.mdpi.com/1999-4915/13/4/554Chikungunyapathological findingsspontaneous abortionPCR confirmed infection |
| spellingShingle | Natália Salomão Michelle Brendolin Kíssila Rabelo Mayumi Wakimoto Ana Maria de Filippis Flavia dos Santos Maria Elizabeth Moreira Carlos Alberto Basílio-de-Oliveira Elyzabeth Avvad-Portari Marciano Paes Patrícia Brasil Spontaneous Abortion and Chikungunya Infection: Pathological Findings Viruses Chikungunya pathological findings spontaneous abortion PCR confirmed infection |
| title | Spontaneous Abortion and Chikungunya Infection: Pathological Findings |
| title_full | Spontaneous Abortion and Chikungunya Infection: Pathological Findings |
| title_fullStr | Spontaneous Abortion and Chikungunya Infection: Pathological Findings |
| title_full_unstemmed | Spontaneous Abortion and Chikungunya Infection: Pathological Findings |
| title_short | Spontaneous Abortion and Chikungunya Infection: Pathological Findings |
| title_sort | spontaneous abortion and chikungunya infection pathological findings |
| topic | Chikungunya pathological findings spontaneous abortion PCR confirmed infection |
| url | https://www.mdpi.com/1999-4915/13/4/554 |
| work_keys_str_mv | AT nataliasalomao spontaneousabortionandchikungunyainfectionpathologicalfindings AT michellebrendolin spontaneousabortionandchikungunyainfectionpathologicalfindings AT kissilarabelo spontaneousabortionandchikungunyainfectionpathologicalfindings AT mayumiwakimoto spontaneousabortionandchikungunyainfectionpathologicalfindings AT anamariadefilippis spontaneousabortionandchikungunyainfectionpathologicalfindings AT flaviadossantos spontaneousabortionandchikungunyainfectionpathologicalfindings AT mariaelizabethmoreira spontaneousabortionandchikungunyainfectionpathologicalfindings AT carlosalbertobasiliodeoliveira spontaneousabortionandchikungunyainfectionpathologicalfindings AT elyzabethavvadportari spontaneousabortionandchikungunyainfectionpathologicalfindings AT marcianopaes spontaneousabortionandchikungunyainfectionpathologicalfindings AT patriciabrasil spontaneousabortionandchikungunyainfectionpathologicalfindings |