A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters
A novel ratiometric fluorescent immunoassay was developed based on silver nanoparticles (AgNPs) for the sensitive determination of dibutyl phthalate (DBP). In the detection system, AgNPs were labeled on the secondary antibody (AgNPs@Ab<sub>2</sub>) for signal amplification, which aimed t...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/2/125 |
| _version_ | 1797482182544982016 |
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| author | Hui Meng Nannan Yao Kun Zeng Nuanfei Zhu Yue Wang Biying Zhao Zhen Zhang |
| author_facet | Hui Meng Nannan Yao Kun Zeng Nuanfei Zhu Yue Wang Biying Zhao Zhen Zhang |
| author_sort | Hui Meng |
| collection | DOAJ |
| description | A novel ratiometric fluorescent immunoassay was developed based on silver nanoparticles (AgNPs) for the sensitive determination of dibutyl phthalate (DBP). In the detection system, AgNPs were labeled on the secondary antibody (AgNPs@Ab<sub>2</sub>) for signal amplification, which aimed to regulate the H<sub>2</sub>O<sub>2</sub> concentrations. When AgNPs-Ab<sub>2</sub> and antigen–primary antibody (Ab<sub>1</sub>) were linked by specific recognition, the blue fluorescence of Scopoletin (SC) could be effectively quenched by the H<sub>2</sub>O<sub>2</sub> added while the red fluorescence of Amplex Red (AR) was generated. Under the optimized conditions, the calculated detection of limit (LOD, 90% inhibition) reached 0.86 ng/mL with a wide linear range of 2.31–66.84 ng/mL, which was approximately eleven times lower than that by HRP-based traditional ELISA with the same antibody. Meanwhile, it could improve the inherent built-in rectification to the environment by the combination of the dual-output ratiometric fluorescence assays with ELISA, which also enhanced the accuracy and precision (recoveries, 87.20–106.62%; CV, 2.57–6.54%), indicating it can be applied to investigate the concentration of DBP in water samples. |
| first_indexed | 2024-03-09T22:27:36Z |
| format | Article |
| id | doaj.art-03a94a9b889e444b85929edfa258535b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T22:27:36Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-03a94a9b889e444b85929edfa258535b2023-11-23T19:01:44ZengMDPI AGBiosensors2079-63742022-02-0112212510.3390/bios12020125A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental WatersHui Meng0Nannan Yao1Kun Zeng2Nuanfei Zhu3Yue Wang4Biying Zhao5Zhen Zhang6School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaInternational Genome Center, Jiangsu University, Zhenjiang 212013, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaA novel ratiometric fluorescent immunoassay was developed based on silver nanoparticles (AgNPs) for the sensitive determination of dibutyl phthalate (DBP). In the detection system, AgNPs were labeled on the secondary antibody (AgNPs@Ab<sub>2</sub>) for signal amplification, which aimed to regulate the H<sub>2</sub>O<sub>2</sub> concentrations. When AgNPs-Ab<sub>2</sub> and antigen–primary antibody (Ab<sub>1</sub>) were linked by specific recognition, the blue fluorescence of Scopoletin (SC) could be effectively quenched by the H<sub>2</sub>O<sub>2</sub> added while the red fluorescence of Amplex Red (AR) was generated. Under the optimized conditions, the calculated detection of limit (LOD, 90% inhibition) reached 0.86 ng/mL with a wide linear range of 2.31–66.84 ng/mL, which was approximately eleven times lower than that by HRP-based traditional ELISA with the same antibody. Meanwhile, it could improve the inherent built-in rectification to the environment by the combination of the dual-output ratiometric fluorescence assays with ELISA, which also enhanced the accuracy and precision (recoveries, 87.20–106.62%; CV, 2.57–6.54%), indicating it can be applied to investigate the concentration of DBP in water samples.https://www.mdpi.com/2079-6374/12/2/125immunoassayantibodydibutyl phthalatescopoletinamplex redsilver nanoparticles |
| spellingShingle | Hui Meng Nannan Yao Kun Zeng Nuanfei Zhu Yue Wang Biying Zhao Zhen Zhang A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters Biosensors immunoassay antibody dibutyl phthalate scopoletin amplex red silver nanoparticles |
| title | A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters |
| title_full | A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters |
| title_fullStr | A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters |
| title_full_unstemmed | A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters |
| title_short | A Novel Enzyme-Free Ratiometric Fluorescence Immunoassay Based on Silver Nanoparticles for the Detection of Dibutyl Phthalate from Environmental Waters |
| title_sort | novel enzyme free ratiometric fluorescence immunoassay based on silver nanoparticles for the detection of dibutyl phthalate from environmental waters |
| topic | immunoassay antibody dibutyl phthalate scopoletin amplex red silver nanoparticles |
| url | https://www.mdpi.com/2079-6374/12/2/125 |
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