Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China
New Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in Salmonella species prior to this study. Here we describe the first identification of a Salmonella Rissen strain SR33 carrying blaNDM-13. The aim of this study was to molecularly...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.936649/full |
| _version_ | 1828327650997829632 |
|---|---|
| author | Yulan Huang Xiaobo Ma Xiaobo Ma Shihan Zeng Liang Fu Heping Xu Heping Xu Xiaoyan Li |
| author_facet | Yulan Huang Xiaobo Ma Xiaobo Ma Shihan Zeng Liang Fu Heping Xu Heping Xu Xiaoyan Li |
| author_sort | Yulan Huang |
| collection | DOAJ |
| description | New Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in Salmonella species prior to this study. Here we describe the first identification of a Salmonella Rissen strain SR33 carrying blaNDM-13. The aim of this study was to molecularly characterize SR33’s antimicrobial resistance and virulence features as well as investigate the genetic environment of blaNDM-13. The Salmonella Rissen SR33 strain was isolated from a patient with fever and diarrhea. SR33 belonged to ST469, and it was found to be multidrug-resistant (MDR) and to carry many virulence genes. Phylogenetic analysis showed that SR33 shared a close relationship with most of the Chinese S. Rissen ST469 strains. blaNDM-13 was located in a transmissible IncI1 plasmid pNDM13-SR33. Sequence analysis of blaNDM-13-positive genomes downloaded from GenBank revealed that a genetic context (ΔISAba125-blaNDM-13-bleMBL-trpF) and a hybrid promoter (consisting of −35 sequences provided by ISAba125 and −10 sequences) were conserved. ISAba125 was truncated by IS1294 in three plasmids carrying blaNDM-13, including pNDM13-SR33. To our knowledge, this is the first report of blaNDM-13 carried by Salmonella. The emergence of blaNDM-13 in a clinical MDR S. Rissen ST469 strain highlights the critical need for monitoring and controlling the dissemination of blaNDM-13. blaNDM-13 carried by a transmissible IncI1 plasmid may result in an increased risk of blaNDM-13 transmission. IS1294 may be involved in the movement of blaNDM-13. |
| first_indexed | 2024-04-13T19:58:52Z |
| format | Article |
| id | doaj.art-03adbda76fc047b0a7287977c87c7c6e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T19:58:52Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-03adbda76fc047b0a7287977c87c7c6e2022-12-22T02:32:16ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.936649936649Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in ChinaYulan Huang0Xiaobo Ma1Xiaobo Ma2Shihan Zeng3Liang Fu4Heping Xu5Heping Xu6Xiaoyan Li7Department of Clinical Laboratory, Fifth Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Clinical Laboratory, the First Affiliated Hospital of Xiamen University (Xiamen Key Laboratory of Genetic Testing), School of medicine, Xiamen University, Xiamen, ChinaSchool of Public Health, Xiamen University, Xiamen, ChinaDepartment of Clinical Laboratory, Fifth Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Clinical Laboratory, Fifth Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Clinical Laboratory, the First Affiliated Hospital of Xiamen University (Xiamen Key Laboratory of Genetic Testing), School of medicine, Xiamen University, Xiamen, ChinaSchool of Public Health, Xiamen University, Xiamen, ChinaDepartment of Clinical Laboratory, Fifth Affiliated Hospital, Southern Medical University, Guangzhou, ChinaNew Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in Salmonella species prior to this study. Here we describe the first identification of a Salmonella Rissen strain SR33 carrying blaNDM-13. The aim of this study was to molecularly characterize SR33’s antimicrobial resistance and virulence features as well as investigate the genetic environment of blaNDM-13. The Salmonella Rissen SR33 strain was isolated from a patient with fever and diarrhea. SR33 belonged to ST469, and it was found to be multidrug-resistant (MDR) and to carry many virulence genes. Phylogenetic analysis showed that SR33 shared a close relationship with most of the Chinese S. Rissen ST469 strains. blaNDM-13 was located in a transmissible IncI1 plasmid pNDM13-SR33. Sequence analysis of blaNDM-13-positive genomes downloaded from GenBank revealed that a genetic context (ΔISAba125-blaNDM-13-bleMBL-trpF) and a hybrid promoter (consisting of −35 sequences provided by ISAba125 and −10 sequences) were conserved. ISAba125 was truncated by IS1294 in three plasmids carrying blaNDM-13, including pNDM13-SR33. To our knowledge, this is the first report of blaNDM-13 carried by Salmonella. The emergence of blaNDM-13 in a clinical MDR S. Rissen ST469 strain highlights the critical need for monitoring and controlling the dissemination of blaNDM-13. blaNDM-13 carried by a transmissible IncI1 plasmid may result in an increased risk of blaNDM-13 transmission. IS1294 may be involved in the movement of blaNDM-13.https://www.frontiersin.org/articles/10.3389/fcimb.2022.936649/fullblaNDM-13Salmonella RissenST469ISAba125IS1294 |
| spellingShingle | Yulan Huang Xiaobo Ma Xiaobo Ma Shihan Zeng Liang Fu Heping Xu Heping Xu Xiaoyan Li Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China Frontiers in Cellular and Infection Microbiology blaNDM-13 Salmonella Rissen ST469 ISAba125 IS1294 |
| title | Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China |
| title_full | Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China |
| title_fullStr | Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China |
| title_full_unstemmed | Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China |
| title_short | Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China |
| title_sort | emergence of a salmonella rissen st469 clinical isolate carrying blandm 13 in china |
| topic | blaNDM-13 Salmonella Rissen ST469 ISAba125 IS1294 |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.936649/full |
| work_keys_str_mv | AT yulanhuang emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT xiaoboma emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT xiaoboma emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT shihanzeng emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT liangfu emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT hepingxu emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT hepingxu emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina AT xiaoyanli emergenceofasalmonellarissenst469clinicalisolatecarryingblandm13inchina |