Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis
Decabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a <i>Rhodococcus ruber</i> strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong’an Bay, Xiamen. Under laboratory condition...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/7/1441 |
| _version_ | 1797416840241086464 |
|---|---|
| author | Hao Xu Qingtao Cai Qiuying An Chen Tang Wanpeng Wang Guangshun Wang Wanting You Dongbei Guo Ran Zhao |
| author_facet | Hao Xu Qingtao Cai Qiuying An Chen Tang Wanpeng Wang Guangshun Wang Wanting You Dongbei Guo Ran Zhao |
| author_sort | Hao Xu |
| collection | DOAJ |
| description | Decabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a <i>Rhodococcus ruber</i> strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong’an Bay, Xiamen. Under laboratory conditions, the strain’s optimal growth temperature, pH, and salinity are 45 °C, 7.0, and 0–2.5%, respectively. Scanning electron microscopy (SEM) analysis shows that TAW-CT127 is damaged when grown in manual marine culture (MMC) medium with BDE-209 as the sole carbon source instead of eutrophic conditions. In the dark, under the conditions of 28 °C, 160 rpm, and 3 g/L (wet weight) TAW-CT127, the degradation rate of 50 mg/L BDE-209 is 81.07%. The intermediate metabolites are hexabromo-, octabromo-, and nonabromo-diphenyl ethers. Through whole-genome sequencing, multiple dehalogenases were found in the genome of TAW-CT127; these may be involved in the production of lower-brominated diphenyl ethers. Additionally, biphenyl-2,3-dioxygenase (BDO) in TAW-CT127 may catalyze the debromination reaction of BDE-209. Our research provides a new high-efficiency strain for bioremediation of BDE-209 pollution, and lays the foundation for the preliminary exploration of genes associated with BDE-209 degradation. |
| first_indexed | 2024-03-09T06:09:59Z |
| format | Article |
| id | doaj.art-03adc80853b745dab8938ca406b0e2a7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:09:59Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-03adc80853b745dab8938ca406b0e2a72023-12-03T11:59:28ZengMDPI AGMicroorganisms2076-26072022-07-01107144110.3390/microorganisms10071441Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome AnalysisHao Xu0Qingtao Cai1Qiuying An2Chen Tang3Wanpeng Wang4Guangshun Wang5Wanting You6Dongbei Guo7Ran Zhao8State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaThe Second People’s Hospital of Futian District in Shenzhen, Shenzhen 518000, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaDecabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a <i>Rhodococcus ruber</i> strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong’an Bay, Xiamen. Under laboratory conditions, the strain’s optimal growth temperature, pH, and salinity are 45 °C, 7.0, and 0–2.5%, respectively. Scanning electron microscopy (SEM) analysis shows that TAW-CT127 is damaged when grown in manual marine culture (MMC) medium with BDE-209 as the sole carbon source instead of eutrophic conditions. In the dark, under the conditions of 28 °C, 160 rpm, and 3 g/L (wet weight) TAW-CT127, the degradation rate of 50 mg/L BDE-209 is 81.07%. The intermediate metabolites are hexabromo-, octabromo-, and nonabromo-diphenyl ethers. Through whole-genome sequencing, multiple dehalogenases were found in the genome of TAW-CT127; these may be involved in the production of lower-brominated diphenyl ethers. Additionally, biphenyl-2,3-dioxygenase (BDO) in TAW-CT127 may catalyze the debromination reaction of BDE-209. Our research provides a new high-efficiency strain for bioremediation of BDE-209 pollution, and lays the foundation for the preliminary exploration of genes associated with BDE-209 degradation.https://www.mdpi.com/2076-2607/10/7/1441BDE-209<i>Rhodococcus ruber</i>TAW-CT127aerobic degradationgenomics |
| spellingShingle | Hao Xu Qingtao Cai Qiuying An Chen Tang Wanpeng Wang Guangshun Wang Wanting You Dongbei Guo Ran Zhao Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis Microorganisms BDE-209 <i>Rhodococcus ruber</i> TAW-CT127 aerobic degradation genomics |
| title | Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis |
| title_full | Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis |
| title_fullStr | Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis |
| title_full_unstemmed | Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis |
| title_short | Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis |
| title_sort | aerobic degradation characteristics of decabromodiphenyl ether through i rhodococcus ruber i taw ct127 and its preliminary genome analysis |
| topic | BDE-209 <i>Rhodococcus ruber</i> TAW-CT127 aerobic degradation genomics |
| url | https://www.mdpi.com/2076-2607/10/7/1441 |
| work_keys_str_mv | AT haoxu aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT qingtaocai aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT qiuyingan aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT chentang aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT wanpengwang aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT guangshunwang aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT wantingyou aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT dongbeiguo aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis AT ranzhao aerobicdegradationcharacteristicsofdecabromodiphenyletherthroughirhodococcusruberitawct127anditspreliminarygenomeanalysis |