Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis
AIM: To investigate the potential interactions of thymic stromal lymphopoietin (TSLP) with interleukin-4 (IL-4) in adaptive immunity during fungal keratitis (FK). METHODS: An FK mouse model was induced with Aspergillus fumigatus (AF) hyphal infection. Mice were divided into several groups: untreated...
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| Format: | Article |
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Press of International Journal of Ophthalmology (IJO PRESS)
2021-10-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2021/10/20211002.pdf |
| _version_ | 1818716246951067648 |
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| author | Chen Chen Chen-Yang Dai Fang Han Jia-Yin Wu Lin Sun Xin-Yi Wu |
| author_facet | Chen Chen Chen-Yang Dai Fang Han Jia-Yin Wu Lin Sun Xin-Yi Wu |
| author_sort | Chen Chen |
| collection | DOAJ |
| description | AIM: To investigate the potential interactions of thymic stromal lymphopoietin (TSLP) with interleukin-4 (IL-4) in adaptive immunity during fungal keratitis (FK). METHODS: An FK mouse model was induced with Aspergillus fumigatus (AF) hyphal infection. Mice were divided into several groups: untreated, phosphate buffer saline (PBS), infected with AF, and pretreated with a scrambled siRNA, a TSLP-specific siRNA (TSLP siRNA), murine recombinant TSLP (rTSLP), immunoglobulin G (IgG), murine recombinant IFN (rIFN-γ), murine recombinant IL-4 (rIL-4), rIL-13, murine recombinant IL-17A (rIL-17A), and murine recombinant IL-17F (rIL-17F) groups. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) or Western blot were performed to determine mRNA and protein levels in the inflamed cornea. Cytokine locations were observed by immunofluoresence staining after AF hyphal infection. RESULTS: Compared to those in the untreated group, TSLP and T helper type 1 (Th1) cytokine levels in the AF group were upregulated at 24h post infection (hpi), and those of T helper type 2 (Th2) and T helper type 17 (Th17) cytokines were increased at 5d post infection (dpi). Th2 cytokine levels were decreased in the TSLP siRNA-pretreated group and increased in the rTSLP-pretreated group compared with the AF group. The TSLP level was increased in the rIL-4-pretreated group, but there were no significant changes among the other groups. Immunofluorescence staining showed cytokine locations after AF hyphal infection. CONCLUSION: TSLP induces a Th2 immune response and promots Th2 T cell differentiation in vivo. IL-4 promotes TSLP secretion. Therefore, TSLP with IL-4 regulates adaptive immunity in FK. |
| first_indexed | 2024-12-17T19:16:13Z |
| format | Article |
| id | doaj.art-03b061a749f84ef2bf32313d0ab73048 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-17T19:16:13Z |
| publishDate | 2021-10-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-03b061a749f84ef2bf32313d0ab730482022-12-21T21:35:45ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982021-10-0114101473148310.18240/ijo.2021.10.0220211002Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitisChen Chen0Chen-Yang Dai1Fang Han2Jia-Yin Wu3Lin Sun4Xin-Yi Wu5Xin-Yi Wu. Department of Ophthalmology, Qilu Hospital of Shandong University, Wenhua Xi Road 107, Jinan 250012, Shandong Province, China. xywu8868@163.comDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, ChinaDepartment of Ophthalmology, Clinical Medical College of Shandong University, Jinan 250012, Shandong Province, China; Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China; Key Laboratory of Cardiovascular Remodeling and Function Research, Shandong University, Jinan 250012, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, ChinaDepartment of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, ChinaAIM: To investigate the potential interactions of thymic stromal lymphopoietin (TSLP) with interleukin-4 (IL-4) in adaptive immunity during fungal keratitis (FK). METHODS: An FK mouse model was induced with Aspergillus fumigatus (AF) hyphal infection. Mice were divided into several groups: untreated, phosphate buffer saline (PBS), infected with AF, and pretreated with a scrambled siRNA, a TSLP-specific siRNA (TSLP siRNA), murine recombinant TSLP (rTSLP), immunoglobulin G (IgG), murine recombinant IFN (rIFN-γ), murine recombinant IL-4 (rIL-4), rIL-13, murine recombinant IL-17A (rIL-17A), and murine recombinant IL-17F (rIL-17F) groups. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) or Western blot were performed to determine mRNA and protein levels in the inflamed cornea. Cytokine locations were observed by immunofluoresence staining after AF hyphal infection. RESULTS: Compared to those in the untreated group, TSLP and T helper type 1 (Th1) cytokine levels in the AF group were upregulated at 24h post infection (hpi), and those of T helper type 2 (Th2) and T helper type 17 (Th17) cytokines were increased at 5d post infection (dpi). Th2 cytokine levels were decreased in the TSLP siRNA-pretreated group and increased in the rTSLP-pretreated group compared with the AF group. The TSLP level was increased in the rIL-4-pretreated group, but there were no significant changes among the other groups. Immunofluorescence staining showed cytokine locations after AF hyphal infection. CONCLUSION: TSLP induces a Th2 immune response and promots Th2 T cell differentiation in vivo. IL-4 promotes TSLP secretion. Therefore, TSLP with IL-4 regulates adaptive immunity in FK.http://ies.ijo.cn/en_publish/2021/10/20211002.pdfaspergillus fumigatuskeratitisthymic stromal lymphopoietinth2 immune responseinterleukin-4 |
| spellingShingle | Chen Chen Chen-Yang Dai Fang Han Jia-Yin Wu Lin Sun Xin-Yi Wu Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis International Journal of Ophthalmology aspergillus fumigatus keratitis thymic stromal lymphopoietin th2 immune response interleukin-4 |
| title | Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis |
| title_full | Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis |
| title_fullStr | Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis |
| title_full_unstemmed | Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis |
| title_short | Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis |
| title_sort | interactions of thymic stromal lymphopoietin with interleukin 4 in adaptive immunity during aspergillus fumigatus keratitis |
| topic | aspergillus fumigatus keratitis thymic stromal lymphopoietin th2 immune response interleukin-4 |
| url | http://ies.ijo.cn/en_publish/2021/10/20211002.pdf |
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