A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products
The aim of this study is to combine advanced GC-MS and metabolite identification in a robust and repeatable technology platform to characterize the metabolome of buffalo milk and mozzarella cheese. The study utilized eleven dairies located in a protected designation of origin (PDO) region and nine d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/2/304 |
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| author | Angela Salzano Gelsomina Manganiello Gianluca Neglia Francesco Vinale Donato De Nicola Michael D’Occhio Giuseppe Campanile |
| author_facet | Angela Salzano Gelsomina Manganiello Gianluca Neglia Francesco Vinale Donato De Nicola Michael D’Occhio Giuseppe Campanile |
| author_sort | Angela Salzano |
| collection | DOAJ |
| description | The aim of this study is to combine advanced GC-MS and metabolite identification in a robust and repeatable technology platform to characterize the metabolome of buffalo milk and mozzarella cheese. The study utilized eleven dairies located in a protected designation of origin (PDO) region and nine dairies located in non-PDO region in Italy. Samples of raw milk (100 mL) and mozzarella cheese (100 g) were obtained from each dairy. A total of 185 metabolites were consistently detected in both milk and mozzarella cheese. The PLS-DA score plots clearly differentiated PDO and non-PDO milk and mozzarella samples. For milk samples, it was possible to divide metabolites into two classes according to region: those with lower concentrations in PDO samples (galactopyranoside, hydroxybuthyric acid, allose, citric acid) and those with lower concentrations in non-PDO samples (talopyranose, pantothenic acid, mannobiose, etc.,). The same was observed for mozzarella samples with the proportion of some metabolites (talopyranose, 2, 3-dihydroxypropyl icosanoate, etc.,) higher in PDO samples while others (tagatose, lactic acid dimer, ribitol, etc.,) higher in non-PDO samples. The findings establish the utility of GC-MS together with mass spectral libraries as a powerful technology platform to determine the authenticity, and create market protection, for “Mozzarella di Bufala Campana.” |
| first_indexed | 2024-12-10T23:50:45Z |
| format | Article |
| id | doaj.art-03b09b0d96354772bc501222d77740bd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T23:50:45Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-03b09b0d96354772bc501222d77740bd2022-12-22T01:28:46ZengMDPI AGMolecules1420-30492020-01-0125230410.3390/molecules25020304molecules25020304A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy ProductsAngela Salzano0Gelsomina Manganiello1Gianluca Neglia2Francesco Vinale3Donato De Nicola4Michael D’Occhio5Giuseppe Campanile6Dipartimento di Medicina Veterinaria e Produzioni Animali, University of Naples “Federico II”, 80137 Naples, ItalyDipartimento di Agraria, University of Naples “Federico II”, 80055 Portici (NA), ItalyDipartimento di Medicina Veterinaria e Produzioni Animali, University of Naples “Federico II”, 80137 Naples, ItalyDipartimento di Medicina Veterinaria e Produzioni Animali, University of Naples “Federico II”, 80137 Naples, ItalyDipartimento di Medicina Veterinaria e Produzioni Animali, University of Naples “Federico II”, 80137 Naples, ItalyFaculty of Science, The University of Sydney, Sydney, NSW 2006, AustraliaDipartimento di Medicina Veterinaria e Produzioni Animali, University of Naples “Federico II”, 80137 Naples, ItalyThe aim of this study is to combine advanced GC-MS and metabolite identification in a robust and repeatable technology platform to characterize the metabolome of buffalo milk and mozzarella cheese. The study utilized eleven dairies located in a protected designation of origin (PDO) region and nine dairies located in non-PDO region in Italy. Samples of raw milk (100 mL) and mozzarella cheese (100 g) were obtained from each dairy. A total of 185 metabolites were consistently detected in both milk and mozzarella cheese. The PLS-DA score plots clearly differentiated PDO and non-PDO milk and mozzarella samples. For milk samples, it was possible to divide metabolites into two classes according to region: those with lower concentrations in PDO samples (galactopyranoside, hydroxybuthyric acid, allose, citric acid) and those with lower concentrations in non-PDO samples (talopyranose, pantothenic acid, mannobiose, etc.,). The same was observed for mozzarella samples with the proportion of some metabolites (talopyranose, 2, 3-dihydroxypropyl icosanoate, etc.,) higher in PDO samples while others (tagatose, lactic acid dimer, ribitol, etc.,) higher in non-PDO samples. The findings establish the utility of GC-MS together with mass spectral libraries as a powerful technology platform to determine the authenticity, and create market protection, for “Mozzarella di Bufala Campana.”https://www.mdpi.com/1420-3049/25/2/304metabolomegc-msbuffalomilkmozzarellaauthenticity |
| spellingShingle | Angela Salzano Gelsomina Manganiello Gianluca Neglia Francesco Vinale Donato De Nicola Michael D’Occhio Giuseppe Campanile A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products Molecules metabolome gc-ms buffalo milk mozzarella authenticity |
| title | A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products |
| title_full | A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products |
| title_fullStr | A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products |
| title_full_unstemmed | A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products |
| title_short | A Preliminary Study on Metabolome Profiles of Buffalo Milk and Corresponding Mozzarella Cheese: Safeguarding the Authenticity and Traceability of Protected Status Buffalo Dairy Products |
| title_sort | preliminary study on metabolome profiles of buffalo milk and corresponding mozzarella cheese safeguarding the authenticity and traceability of protected status buffalo dairy products |
| topic | metabolome gc-ms buffalo milk mozzarella authenticity |
| url | https://www.mdpi.com/1420-3049/25/2/304 |
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