IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis
Background: In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/215 |
| _version_ | 1798027370049830912 |
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| author | S Tolouei K Ghaedi A Khamesipour M Akbari M Baghaei SJ Hasheminia M Narimani SH Hejazi |
| author_facet | S Tolouei K Ghaedi A Khamesipour M Akbari M Baghaei SJ Hasheminia M Narimani SH Hejazi |
| author_sort | S Tolouei |
| collection | DOAJ |
| description | Background: In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore whether IL-23 or IL-27 plays any role in healing process of cutaneous lesions induced by L. major.
Methods: Twenty patients resident in Isfahan Province, with healing or non-healing form of cutaneous leishmaniasis lesion caused by Leishmania major participated in this study. In vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated using ELISA method. Patient with healing form of lesion received no treatment and patient with non-healing form of lesion received at least 2 courses of glucantime.
Results: The mean production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form of lesion. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of CL was significantly higher than non- healing form of CL (P < 0.001).
Conclusion: IL-23 and IL-27 might play a role in human leishmaniasis and further studies are needed to understand the role of IL-23 and IL-27 in leishmaniasis. |
| first_indexed | 2024-04-11T18:50:21Z |
| format | Article |
| id | doaj.art-03b10110804946209cfa8070b499053f |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T18:50:21Z |
| publishDate | 2012-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-03b10110804946209cfa8070b499053f2022-12-22T04:08:23ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2012-03-0171IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous LeishmaniasisS Tolouei0K Ghaedi1A Khamesipour2M Akbari3M Baghaei4SJ Hasheminia5M Narimani6SH Hejazi7Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Biology, School of Sciences, University of Isfahan, Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, IranSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Biology, Faculty of Basic Sciences, Islamic Azad University Shahrekord Branch, Shahrekord, IranDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranSkin Disease and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore whether IL-23 or IL-27 plays any role in healing process of cutaneous lesions induced by L. major. Methods: Twenty patients resident in Isfahan Province, with healing or non-healing form of cutaneous leishmaniasis lesion caused by Leishmania major participated in this study. In vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated using ELISA method. Patient with healing form of lesion received no treatment and patient with non-healing form of lesion received at least 2 courses of glucantime. Results: The mean production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form of lesion. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of CL was significantly higher than non- healing form of CL (P < 0.001). Conclusion: IL-23 and IL-27 might play a role in human leishmaniasis and further studies are needed to understand the role of IL-23 and IL-27 in leishmaniasis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/215IL-23IL-27MacrophagesPromastigoteCutaneous leishmaniasis |
| spellingShingle | S Tolouei K Ghaedi A Khamesipour M Akbari M Baghaei SJ Hasheminia M Narimani SH Hejazi IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis Iranian Journal of Parasitology IL-23 IL-27 Macrophages Promastigote Cutaneous leishmaniasis |
| title | IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis |
| title_full | IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis |
| title_fullStr | IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis |
| title_full_unstemmed | IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis |
| title_short | IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis |
| title_sort | il 23 and il 27 levels in macrophages collected from peripheral blood of patients with healing vs non healing form of cutaneous leishmaniasis |
| topic | IL-23 IL-27 Macrophages Promastigote Cutaneous leishmaniasis |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/215 |
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