Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids
The effect of laser irradiation of rats combined with omega-3 polyunsaturated fatty acids (ω-3 PUFA) administration on the proteins oxidative modification and superoxide dismutase and catalase activity in the mitochondrial fraction of the liver was investigated. Animals were irradiated with a 650 nm...
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| Format: | Article |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2022-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2022/05/Ketsa_94_1.pdf |
| _version_ | 1797635646698815488 |
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| author | O. V. Ketsa M. O. Zelinska M. M. Marchenko |
| author_facet | O. V. Ketsa M. O. Zelinska M. M. Marchenko |
| author_sort | O. V. Ketsa |
| collection | DOAJ |
| description | The effect of laser irradiation of rats combined with omega-3 polyunsaturated fatty acids (ω-3 PUFA) administration on the proteins oxidative modification and superoxide dismutase and catalase activity in the mitochondrial fraction of the liver was investigated. Animals were irradiated with a 650 nm laser diode in the abdomen daily for 4 min at the distance of 10 cm from the skin surface. ω-3 PUFA were administered per os at a daily dose of 120 mg/kg of the body weight. Fatty acids in the fish oil were identified by gas chromatography. Animals were divided into five groups (12 animals in each group): I – intact rats (control); II – rats exposed to the daily laser irradiation for 7 or 14 days ; III – rats that received ω-3 PUFA two hours after irradiation; IV – rats that received ω-3 PUFA two hours before irradiation; V – rats that received ω-3 PUFA for 7 days before irradiation. The mitochondrial fraction of rat liver was obtained by differential centrifugation. The increase in the content of protein carbonyl derivatives and a decrease of protein thiol groups in the liver mitochondrial fraction were detected after seven-day laser irradiation of rats. As the duration of irradiation increases, superoxide dismutase and catalase activity was decreased, indicating a depletion of mitochondrial antioxidant reserves. No antioxidant effect was observed when ω-3 PUFA were administrated after laser irradiation and a slight antioxidant effect was shown when ω-3 PUFA were administrated two hours before irradiation. Preliminary seven-day administration ω-3 PUFA before laser irradiation was the most effective, as it reduced the level of protein carbonyl derivatives and O2•--generation, increased proteins SH-groups content and antioxidant enzymes activity. |
| first_indexed | 2024-03-11T12:23:21Z |
| format | Article |
| id | doaj.art-03b138d5c90a4c67a49810492e7bfe2f |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T12:23:21Z |
| publishDate | 2022-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-03b138d5c90a4c67a49810492e7bfe2f2023-11-06T13:21:38ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032022-02-01941445210.15407/ubj94.01.044Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acidsO. V. Ketsa0https://orcid.org/0000-0002-2695-1790M. O. Zelinska1https://orcid.org/0000-0001-6104-0119M. M. Marchenko2https://orcid.org/0000-0001-6104-0119Yuriy Fedkovych Chernivtsi National University, Chernivtsi, UkraineYuriy Fedkovych Chernivtsi National University, Chernivtsi, UkraineYuriy Fedkovych Chernivtsi National University, Chernivtsi, UkraineThe effect of laser irradiation of rats combined with omega-3 polyunsaturated fatty acids (ω-3 PUFA) administration on the proteins oxidative modification and superoxide dismutase and catalase activity in the mitochondrial fraction of the liver was investigated. Animals were irradiated with a 650 nm laser diode in the abdomen daily for 4 min at the distance of 10 cm from the skin surface. ω-3 PUFA were administered per os at a daily dose of 120 mg/kg of the body weight. Fatty acids in the fish oil were identified by gas chromatography. Animals were divided into five groups (12 animals in each group): I – intact rats (control); II – rats exposed to the daily laser irradiation for 7 or 14 days ; III – rats that received ω-3 PUFA two hours after irradiation; IV – rats that received ω-3 PUFA two hours before irradiation; V – rats that received ω-3 PUFA for 7 days before irradiation. The mitochondrial fraction of rat liver was obtained by differential centrifugation. The increase in the content of protein carbonyl derivatives and a decrease of protein thiol groups in the liver mitochondrial fraction were detected after seven-day laser irradiation of rats. As the duration of irradiation increases, superoxide dismutase and catalase activity was decreased, indicating a depletion of mitochondrial antioxidant reserves. No antioxidant effect was observed when ω-3 PUFA were administrated after laser irradiation and a slight antioxidant effect was shown when ω-3 PUFA were administrated two hours before irradiation. Preliminary seven-day administration ω-3 PUFA before laser irradiation was the most effective, as it reduced the level of protein carbonyl derivatives and O2•--generation, increased proteins SH-groups content and antioxidant enzymes activity.http://ukrbiochemjournal.org/wp-content/uploads/2022/05/Ketsa_94_1.pdfcarbonyl derivativeslaser irradiationliver mitochondriash-groupssuperoxide dismutase and catalase activitysuperoxide radicalω-3 polyunsaturated fatty acids |
| spellingShingle | O. V. Ketsa M. O. Zelinska M. M. Marchenko Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids The Ukrainian Biochemical Journal carbonyl derivatives laser irradiation liver mitochondria sh-groups superoxide dismutase and catalase activity superoxide radical ω-3 polyunsaturated fatty acids |
| title | Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids |
| title_full | Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids |
| title_fullStr | Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids |
| title_full_unstemmed | Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids |
| title_short | Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids |
| title_sort | proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω 3 polyunsaturated fatty acids |
| topic | carbonyl derivatives laser irradiation liver mitochondria sh-groups superoxide dismutase and catalase activity superoxide radical ω-3 polyunsaturated fatty acids |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2022/05/Ketsa_94_1.pdf |
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