Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780
The aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2012-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/55/3/0116/ |
| _version_ | 1818132646836830208 |
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| author | Kateřina Caltová Miroslav Červinka |
| author_facet | Kateřina Caltová Miroslav Červinka |
| author_sort | Kateřina Caltová |
| collection | DOAJ |
| description | The aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan and etoposide. These cytostatics have a different mechanism of action. To evaluate cytotoxic potential of the tested compounds, the methods measuring various toxicological endpoints were employed including morphological studies, MTT assay, dynamic monitoring of cell proliferation with xCELLigence, cell cycle analysis, caspase 3 activity and expression of proteins involved in cell cycle regulation and cell death. The A270 cell line showed different sensitivity towards the selected cytostatics, the highest cytotoxic effect was associated with paclitaxel and topotecan. |
| first_indexed | 2024-12-11T08:40:09Z |
| format | Article |
| id | doaj.art-03b1cec8689f43168df2bcc711366282 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-11T08:40:09Z |
| publishDate | 2012-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-03b1cec8689f43168df2bcc7113662822022-12-22T01:14:16ZengKarolinum PressActa Medica1211-42861805-96942012-01-0155311612410.14712/18059694.2015.48Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780Kateřina Caltová0Miroslav Červinka1Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Medical Biology and GeneticsCharles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Medical Biology and GeneticsThe aim of our study was to determine the effect of selected cytostatics on a human ovarian cancer cell line A2780 as a model system for ovarian cancer treatment. This cell line is considered cisplatin-sensitive. Panel of tested cytostatics included cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan and etoposide. These cytostatics have a different mechanism of action. To evaluate cytotoxic potential of the tested compounds, the methods measuring various toxicological endpoints were employed including morphological studies, MTT assay, dynamic monitoring of cell proliferation with xCELLigence, cell cycle analysis, caspase 3 activity and expression of proteins involved in cell cycle regulation and cell death. The A270 cell line showed different sensitivity towards the selected cytostatics, the highest cytotoxic effect was associated with paclitaxel and topotecan.https://actamedica.lfhk.cuni.cz/55/3/0116/A2780Cytostatics treatmentChemosensitivity testingCisplatinTopotecan |
| spellingShingle | Kateřina Caltová Miroslav Červinka Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 Acta Medica A2780 Cytostatics treatment Chemosensitivity testing Cisplatin Topotecan |
| title | Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 |
| title_full | Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 |
| title_fullStr | Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 |
| title_full_unstemmed | Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 |
| title_short | Antiproliferative Effects of Selected Chemotherapeutics in Human Ovarian Cancer Cell Line A2780 |
| title_sort | antiproliferative effects of selected chemotherapeutics in human ovarian cancer cell line a2780 |
| topic | A2780 Cytostatics treatment Chemosensitivity testing Cisplatin Topotecan |
| url | https://actamedica.lfhk.cuni.cz/55/3/0116/ |
| work_keys_str_mv | AT katerinacaltova antiproliferativeeffectsofselectedchemotherapeuticsinhumanovariancancercelllinea2780 AT miroslavcervinka antiproliferativeeffectsofselectedchemotherapeuticsinhumanovariancancercelllinea2780 |