Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding
Introduction: Anopheles triannulatus is not incriminated as a vector of malaria transmission in Colombia despite recent reports of infection with Plasmodium spp. in populations related to the northwestern and southeastern lineages. Genetic diversity can delimit information about gene flow and popula...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2018-08-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/4055 |
| _version_ | 1828369243702296576 |
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| author | María Atencia Angie Toro-Cantillo Richard Hoyos-López |
| author_facet | María Atencia Angie Toro-Cantillo Richard Hoyos-López |
| author_sort | María Atencia |
| collection | DOAJ |
| description | Introduction: Anopheles triannulatus is not incriminated as a vector of malaria transmission in Colombia despite recent reports of infection with Plasmodium spp. in populations related to the northwestern and southeastern lineages. Genetic diversity can delimit information about gene flow and population differentiation in localities with malaria.
Objective: To estimate the genetic diversity of An. triannulatus in five municipalities with high and low incidence of malaria in the department of Córdoba.
Materials and methods: The entomological collections were done between August and November, 2016, in Tierralta, Puerto Libertador, Montelíbano, Sahagún, and Planeta Rica. We used the COI barcoding fragment as molecular marker. The genetic analysis included the estimation of genetic parameters such as the diversity haplotype, the genetic structure, the gene flow, the Tajima’s D test, the haplotype network, and the phylogenetic relationship.
Results: We obtained 148 sequences with a length of 655 nucleotides of the COI gene, from which we derived 44 haplotypes. The H2 and H21 haplotypes were the most frequent in the populations. The values of the Tajima’s D test were negative and not significant (p>0.10). The genetic structure
index (FST=0.01427) and the gene flow (Nm=17.27) evidenced no differentiation between sampled populations due to the high exchange of migrants. Using phylogenetic inferences and the haplotype network, we identified one single species without geographic differentiation or lineages in the geographic range studied.
Conclusions: The genetic diversity calculated for An. triannulatus in this context indicated stable populations in constant exchange. |
| first_indexed | 2024-04-14T06:21:07Z |
| format | Article |
| id | doaj.art-03b48b87d1ea4c24bb4e97b5ab568c03 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-04-14T06:21:07Z |
| publishDate | 2018-08-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-03b48b87d1ea4c24bb4e97b5ab568c032022-12-22T02:08:01ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572018-08-0138011712610.7705/biomedica.v38i0.40552174Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcodingMaría Atencia0Angie Toro-Cantillo1Richard Hoyos-López2Grupo de Investigaciones en Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Montería, ColombiaGrupo de Investigaciones en Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Montería, ColombiaGrupo de Investigaciones en Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Montería, ColombiaIntroduction: Anopheles triannulatus is not incriminated as a vector of malaria transmission in Colombia despite recent reports of infection with Plasmodium spp. in populations related to the northwestern and southeastern lineages. Genetic diversity can delimit information about gene flow and population differentiation in localities with malaria. Objective: To estimate the genetic diversity of An. triannulatus in five municipalities with high and low incidence of malaria in the department of Córdoba. Materials and methods: The entomological collections were done between August and November, 2016, in Tierralta, Puerto Libertador, Montelíbano, Sahagún, and Planeta Rica. We used the COI barcoding fragment as molecular marker. The genetic analysis included the estimation of genetic parameters such as the diversity haplotype, the genetic structure, the gene flow, the Tajima’s D test, the haplotype network, and the phylogenetic relationship. Results: We obtained 148 sequences with a length of 655 nucleotides of the COI gene, from which we derived 44 haplotypes. The H2 and H21 haplotypes were the most frequent in the populations. The values of the Tajima’s D test were negative and not significant (p>0.10). The genetic structure index (FST=0.01427) and the gene flow (Nm=17.27) evidenced no differentiation between sampled populations due to the high exchange of migrants. Using phylogenetic inferences and the haplotype network, we identified one single species without geographic differentiation or lineages in the geographic range studied. Conclusions: The genetic diversity calculated for An. triannulatus in this context indicated stable populations in constant exchange.https://www.revistabiomedica.org/index.php/biomedica/article/view/4055Anopheles/genéticavariación genéticahaplotiposflujo génicoColombia |
| spellingShingle | María Atencia Angie Toro-Cantillo Richard Hoyos-López Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding Biomédica: revista del Instituto Nacional de Salud Anopheles/genética variación genética haplotipos flujo génico Colombia |
| title | Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding |
| title_full | Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding |
| title_fullStr | Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding |
| title_full_unstemmed | Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding |
| title_short | Genetic diversity and population structure of Anopheles triannulatus s. l. in the department of Córdoba, Colombia, using DNA barcoding |
| title_sort | genetic diversity and population structure of anopheles triannulatus s l in the department of cordoba colombia using dna barcoding |
| topic | Anopheles/genética variación genética haplotipos flujo génico Colombia |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/4055 |
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