The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study
Devi Windaryanti,1 Christine Sondang Gabriel,1 Ika Wiani Hidayat,1 Achmad Zainuddin,1 Hendra Dian Adhita Dharsono,2 Mieke Hemiawati Satari,3 Dikdik Kurnia1 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Conservative...
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Dove Medical Press
2022-12-01
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| Series: | Advances and Applications in Bioinformatics and Chemistry |
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| Online Access: | https://www.dovepress.com/the-potential-of-24-propylcholestrol-as-antibacterial-oral-bacteria-of-peer-reviewed-fulltext-article-AABC |
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| author | Windaryanti D Gabriel CS Hidayat IW Zainuddin A Dharsono HDA Satari MH Kurnia D |
| author_facet | Windaryanti D Gabriel CS Hidayat IW Zainuddin A Dharsono HDA Satari MH Kurnia D |
| author_sort | Windaryanti D |
| collection | DOAJ |
| description | Devi Windaryanti,1 Christine Sondang Gabriel,1 Ika Wiani Hidayat,1 Achmad Zainuddin,1 Hendra Dian Adhita Dharsono,2 Mieke Hemiawati Satari,3 Dikdik Kurnia1 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Conservative Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Jawa Barat, Indonesia; 3Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Jawa Barat, IndonesiaCorrespondence: Dikdik Kurnia, Jl. Raya Bandung Sumedang km.21, Hegarmanah, Jatinangor, Kabupaten Sumedang, Sumedang, Jawa Barat, 45363, Indonesia, Tel/Fax +022 7794391, Email dikdik.kurnia@unpad.ac.idIntroduction: Uncontrolled biofilm can cause several diseases such as dental caries, gingivitis, and periodontitis. Dental caries is a disease caused by the accumulation of plaque-containing pathogenic bacteria, including Enterococcus faecalis. These bacteria infect the root canals of teeth and colonize to form biofilms. Biofilm inhibition is carried out by interfering with cell wall formation metabolism. MurA enzyme has a role in peptidoglycan biosynthesis of cell walls. Enterococcal surface protein (Esp) is the main contributor of E. faecalis to form biofilms. In addition, inhibition of biofilms by interfering with the quorum sensing (QS) system, suppressing gelatinase virulence factors by blocking autoinducers gelatinase biosynthesis-activating pheromone (GBAP).Purpose: Knowing the potential of Piper betel Linn. compounds as antibacterial in vitro and antibiofilm agents against E. faecalis in silico.Patients and Methods: The compounds were purified by a bioactivity-guided chromatographic method. Antibacterial activity was tested by disc diffusion method, in vitro studies. In silico study, compound P. betel L. was used as the test ligand and compared with positive control fosfomycin, ambuic acid, quercetin, and taxifolin. The proteins used MurA, Esp, GBAP, and gelatinase were docking with the Autodock Vina PyRx 0.8 followed by the PYMOL program and visualized with the Discovery Studio 2020 program.Results: An antibacterial compound was identified 24-propylcholesterol which can inhibit the activity of E. faecalis ATCC 29212 with MIC value of 78.1 μg/mL and MBC value of 156.3 μg/mL. Molecular docking results showed the binding affinity of 24-propylcholesterol with MurA, ESP, GBAP, and gelatinase enzymes was − 7.6, − 8.7, − 5.3, and − 7.9 kcal/mol.Conclusion: 24-propylcholesterol has potential as an antibacterial against E. faecalis and as an antibiofilm through in silico inhibition of QS. However, further research is needed in vitro and in vivo to determine the effectiveness of these compounds as antibacterial and antibiofilm.Keywords: Piper betel Linn, 24-propylcholesterol, biofilm, MurA, QS |
| first_indexed | 2024-04-11T05:31:04Z |
| format | Article |
| id | doaj.art-03b4a40f33064192bf3f84e8c2ae89c9 |
| institution | Directory Open Access Journal |
| issn | 1178-6949 |
| language | English |
| last_indexed | 2024-04-11T05:31:04Z |
| publishDate | 2022-12-01 |
| publisher | Dove Medical Press |
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| series | Advances and Applications in Bioinformatics and Chemistry |
| spelling | doaj.art-03b4a40f33064192bf3f84e8c2ae89c92022-12-22T18:12:17ZengDove Medical PressAdvances and Applications in Bioinformatics and Chemistry1178-69492022-12-01Volume 159911180553The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico StudyWindaryanti DGabriel CSHidayat IWZainuddin ADharsono HDASatari MHKurnia DDevi Windaryanti,1 Christine Sondang Gabriel,1 Ika Wiani Hidayat,1 Achmad Zainuddin,1 Hendra Dian Adhita Dharsono,2 Mieke Hemiawati Satari,3 Dikdik Kurnia1 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Conservative Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Jawa Barat, Indonesia; 3Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Jawa Barat, IndonesiaCorrespondence: Dikdik Kurnia, Jl. Raya Bandung Sumedang km.21, Hegarmanah, Jatinangor, Kabupaten Sumedang, Sumedang, Jawa Barat, 45363, Indonesia, Tel/Fax +022 7794391, Email dikdik.kurnia@unpad.ac.idIntroduction: Uncontrolled biofilm can cause several diseases such as dental caries, gingivitis, and periodontitis. Dental caries is a disease caused by the accumulation of plaque-containing pathogenic bacteria, including Enterococcus faecalis. These bacteria infect the root canals of teeth and colonize to form biofilms. Biofilm inhibition is carried out by interfering with cell wall formation metabolism. MurA enzyme has a role in peptidoglycan biosynthesis of cell walls. Enterococcal surface protein (Esp) is the main contributor of E. faecalis to form biofilms. In addition, inhibition of biofilms by interfering with the quorum sensing (QS) system, suppressing gelatinase virulence factors by blocking autoinducers gelatinase biosynthesis-activating pheromone (GBAP).Purpose: Knowing the potential of Piper betel Linn. compounds as antibacterial in vitro and antibiofilm agents against E. faecalis in silico.Patients and Methods: The compounds were purified by a bioactivity-guided chromatographic method. Antibacterial activity was tested by disc diffusion method, in vitro studies. In silico study, compound P. betel L. was used as the test ligand and compared with positive control fosfomycin, ambuic acid, quercetin, and taxifolin. The proteins used MurA, Esp, GBAP, and gelatinase were docking with the Autodock Vina PyRx 0.8 followed by the PYMOL program and visualized with the Discovery Studio 2020 program.Results: An antibacterial compound was identified 24-propylcholesterol which can inhibit the activity of E. faecalis ATCC 29212 with MIC value of 78.1 μg/mL and MBC value of 156.3 μg/mL. Molecular docking results showed the binding affinity of 24-propylcholesterol with MurA, ESP, GBAP, and gelatinase enzymes was − 7.6, − 8.7, − 5.3, and − 7.9 kcal/mol.Conclusion: 24-propylcholesterol has potential as an antibacterial against E. faecalis and as an antibiofilm through in silico inhibition of QS. However, further research is needed in vitro and in vivo to determine the effectiveness of these compounds as antibacterial and antibiofilm.Keywords: Piper betel Linn, 24-propylcholesterol, biofilm, MurA, QShttps://www.dovepress.com/the-potential-of-24-propylcholestrol-as-antibacterial-oral-bacteria-of-peer-reviewed-fulltext-article-AABCpiper betel linn24-propylcholesterolbiofilmmuraqs |
| spellingShingle | Windaryanti D Gabriel CS Hidayat IW Zainuddin A Dharsono HDA Satari MH Kurnia D The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study Advances and Applications in Bioinformatics and Chemistry piper betel linn 24-propylcholesterol biofilm mura qs |
| title | The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study |
| title_full | The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study |
| title_fullStr | The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study |
| title_full_unstemmed | The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study |
| title_short | The Potential of 24-Propylcholestrol as Antibacterial Oral Bacteria of Enterococcus faecalis ATCC 29212 and Inhibitor Biofilms Formation: in vitro and in silico Study |
| title_sort | potential of 24 propylcholestrol as antibacterial oral bacteria of enterococcus faecalis atcc 29212 and inhibitor biofilms formation in vitro and in silico study |
| topic | piper betel linn 24-propylcholesterol biofilm mura qs |
| url | https://www.dovepress.com/the-potential-of-24-propylcholestrol-as-antibacterial-oral-bacteria-of-peer-reviewed-fulltext-article-AABC |
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