miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma
MicroRNAs are non-coding molecules that act both as regulators of the epigenetic landscape and as biomarkers for diseases, including asthma. In the era of personalized medicine, there is a need for novel disease-associated biomarkers that can help in classifying diseases into phenotypes for treatmen...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.858722/full |
| _version_ | 1811329996355010560 |
|---|---|
| author | José M. Rodrigo-Muñoz José M. Rodrigo-Muñoz Marta Gil-Martínez Clara Lorente-Sorolla Raquel García-Latorre Marcela Valverde-Monge Marcela Valverde-Monge Santiago Quirce Santiago Quirce Joaquín Sastre Joaquín Sastre Victoria del Pozo Victoria del Pozo |
| author_facet | José M. Rodrigo-Muñoz José M. Rodrigo-Muñoz Marta Gil-Martínez Clara Lorente-Sorolla Raquel García-Latorre Marcela Valverde-Monge Marcela Valverde-Monge Santiago Quirce Santiago Quirce Joaquín Sastre Joaquín Sastre Victoria del Pozo Victoria del Pozo |
| author_sort | José M. Rodrigo-Muñoz |
| collection | DOAJ |
| description | MicroRNAs are non-coding molecules that act both as regulators of the epigenetic landscape and as biomarkers for diseases, including asthma. In the era of personalized medicine, there is a need for novel disease-associated biomarkers that can help in classifying diseases into phenotypes for treatment selection. Currently, severe eosinophilic asthma is one of the most widely studied phenotypes in clinical practice, as many patients require higher and higher doses of corticosteroids, which in some cases fail to achieve the desired outcome. Such patients may only benefit from alternative drugs such as biologics, for which novel biomarkers are necessary. The objective of the study was to study the expression of miR-144-3p in order to discover its possible use as a diagnostic biomarker for severe asthma. For this purpose, miR-144-3p was evaluated in airway biopsies and serum from asthmatics and healthy individuals. mRNA was studied in asthmatic biopsies and smooth muscle cells transfected with miR-144-3p mimic. An in silico regulation of miR-144-3p was performed using miRSystem, miRDB, STRING, and ShinyGO for pathway analysis. From our experimental procedures, we found that miR-144-3p is a biomarker associated with asthma severity and corticosteroid treatment. MiR-144-3p is increased in asthmatic lungs, and its presence correlates directly with blood eosinophilia and with the expression of genes involved in asthma pathophysiology in the airways. When studied in serum, this miRNA was increased in severe asthmatics and associated with higher doses of corticosteroids, thereby making it a potential biomarker for severe asthma previously treated with higher doses of corticosteroids. Thus, we can conclude that miR-144-3p is associated with severe diseases in both the airways and serum of asthmatics, and this association is related to corticosteroid treatment. |
| first_indexed | 2024-04-13T15:53:42Z |
| format | Article |
| id | doaj.art-03b8857ed7fa4af3b8e5b5845963a07b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T15:53:42Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-03b8857ed7fa4af3b8e5b5845963a07b2022-12-22T02:40:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-04-011310.3389/fimmu.2022.858722858722miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent AsthmaJosé M. Rodrigo-Muñoz0José M. Rodrigo-Muñoz1Marta Gil-Martínez2Clara Lorente-Sorolla3Raquel García-Latorre4Marcela Valverde-Monge5Marcela Valverde-Monge6Santiago Quirce7Santiago Quirce8Joaquín Sastre9Joaquín Sastre10Victoria del Pozo11Victoria del Pozo12Department of Immunology, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainCentro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), Madrid, SpainDepartment of Immunology, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainDepartment of Immunology, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainDepartment of Immunology, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainCentro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), Madrid, SpainDepartment of Allergy, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainCentro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), Madrid, SpainDepartment of Allergy, Hospital La Paz-Institute for Health Research (IdiPAZ), Madrid, SpainCentro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), Madrid, SpainDepartment of Allergy, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainDepartment of Immunology, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, Madrid, SpainCentro de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), Madrid, SpainMicroRNAs are non-coding molecules that act both as regulators of the epigenetic landscape and as biomarkers for diseases, including asthma. In the era of personalized medicine, there is a need for novel disease-associated biomarkers that can help in classifying diseases into phenotypes for treatment selection. Currently, severe eosinophilic asthma is one of the most widely studied phenotypes in clinical practice, as many patients require higher and higher doses of corticosteroids, which in some cases fail to achieve the desired outcome. Such patients may only benefit from alternative drugs such as biologics, for which novel biomarkers are necessary. The objective of the study was to study the expression of miR-144-3p in order to discover its possible use as a diagnostic biomarker for severe asthma. For this purpose, miR-144-3p was evaluated in airway biopsies and serum from asthmatics and healthy individuals. mRNA was studied in asthmatic biopsies and smooth muscle cells transfected with miR-144-3p mimic. An in silico regulation of miR-144-3p was performed using miRSystem, miRDB, STRING, and ShinyGO for pathway analysis. From our experimental procedures, we found that miR-144-3p is a biomarker associated with asthma severity and corticosteroid treatment. MiR-144-3p is increased in asthmatic lungs, and its presence correlates directly with blood eosinophilia and with the expression of genes involved in asthma pathophysiology in the airways. When studied in serum, this miRNA was increased in severe asthmatics and associated with higher doses of corticosteroids, thereby making it a potential biomarker for severe asthma previously treated with higher doses of corticosteroids. Thus, we can conclude that miR-144-3p is associated with severe diseases in both the airways and serum of asthmatics, and this association is related to corticosteroid treatment.https://www.frontiersin.org/articles/10.3389/fimmu.2022.858722/fullasthmabiomarkersepigeneticsasthma treatmentcorticosteroid |
| spellingShingle | José M. Rodrigo-Muñoz José M. Rodrigo-Muñoz Marta Gil-Martínez Clara Lorente-Sorolla Raquel García-Latorre Marcela Valverde-Monge Marcela Valverde-Monge Santiago Quirce Santiago Quirce Joaquín Sastre Joaquín Sastre Victoria del Pozo Victoria del Pozo miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma Frontiers in Immunology asthma biomarkers epigenetics asthma treatment corticosteroid |
| title | miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma |
| title_full | miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma |
| title_fullStr | miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma |
| title_full_unstemmed | miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma |
| title_short | miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma |
| title_sort | mir 144 3p is a biomarker related to severe corticosteroid dependent asthma |
| topic | asthma biomarkers epigenetics asthma treatment corticosteroid |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.858722/full |
| work_keys_str_mv | AT josemrodrigomunoz mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT josemrodrigomunoz mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT martagilmartinez mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT claralorentesorolla mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT raquelgarcialatorre mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT marcelavalverdemonge mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT marcelavalverdemonge mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT santiagoquirce mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT santiagoquirce mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT joaquinsastre mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT joaquinsastre mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT victoriadelpozo mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma AT victoriadelpozo mir1443pisabiomarkerrelatedtoseverecorticosteroiddependentasthma |