Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens
The aim of this study was to examine whether Salmonella enteritidis (SE) vaccination affects innate immune function and histone modifications responsible for epigenetic reprogramming in the follicular theca of laying hens. White Leghorn laying hens were administered the SE vaccine or phosphate buffe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2019-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/56/4/56_0190034/_html/-char/en |
| _version_ | 1797816980644823040 |
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| author | Ye Kang Takahiro Nii Naoki Isobe Yukinori Yoshimura |
| author_facet | Ye Kang Takahiro Nii Naoki Isobe Yukinori Yoshimura |
| author_sort | Ye Kang |
| collection | DOAJ |
| description | The aim of this study was to examine whether Salmonella enteritidis (SE) vaccination affects innate immune function and histone modifications responsible for epigenetic reprogramming in the follicular theca of laying hens. White Leghorn laying hens were administered the SE vaccine or phosphate buffered saline (PBS; control) one week before sample collection. The largest follicles (F1) were collected for total RNA and histone protein extraction. Gene expression levels of immune molecules (Toll-like receptors [TLRs], cytokines, and avian β-defensins [AvBDs]), and histone modifications in the follicular thecal tissues, were examined using real-time PCR and western blot, respectively. The results showed that the expression levels of TLR1-1, 2-1, 4, and 15 were upregulated by SE vaccination. Although vaccination caused no significant change in cytokine expression, AvBDl, 2, 4, and 7 expression levels were significantly upregulated in the vaccinated group. In addition, the relative density of histone H3-lysine9 dimethylation (H3K9me2) was increased by the vaccination. These results suggest that SE vaccination enhances innate immune functions in the ovary of laying hens, including upregulating TLR and AvBD expression, and is also associated with an increase in histone H3K9me2 in thecal cells. |
| first_indexed | 2024-03-13T08:46:40Z |
| format | Article |
| id | doaj.art-03bcde12bbaf4ac697a0214c2b115b40 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:40Z |
| publishDate | 2019-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-03bcde12bbaf4ac697a0214c2b115b402023-05-30T01:24:51ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862019-10-0156429830710.2141/jpsa.0190034jpsaEffects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying HensYe Kang0Takahiro Nii1Naoki Isobe2Yukinori Yoshimura3Graduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityGraduate School of Biosphere Science, Hiroshima UniversityThe aim of this study was to examine whether Salmonella enteritidis (SE) vaccination affects innate immune function and histone modifications responsible for epigenetic reprogramming in the follicular theca of laying hens. White Leghorn laying hens were administered the SE vaccine or phosphate buffered saline (PBS; control) one week before sample collection. The largest follicles (F1) were collected for total RNA and histone protein extraction. Gene expression levels of immune molecules (Toll-like receptors [TLRs], cytokines, and avian β-defensins [AvBDs]), and histone modifications in the follicular thecal tissues, were examined using real-time PCR and western blot, respectively. The results showed that the expression levels of TLR1-1, 2-1, 4, and 15 were upregulated by SE vaccination. Although vaccination caused no significant change in cytokine expression, AvBDl, 2, 4, and 7 expression levels were significantly upregulated in the vaccinated group. In addition, the relative density of histone H3-lysine9 dimethylation (H3K9me2) was increased by the vaccination. These results suggest that SE vaccination enhances innate immune functions in the ovary of laying hens, including upregulating TLR and AvBD expression, and is also associated with an increase in histone H3K9me2 in thecal cells.https://www.jstage.jst.go.jp/article/jpsa/56/4/56_0190034/_html/-char/enhistone modificationinnate immunitylaying henovarysalmonella enteritidis vaccine |
| spellingShingle | Ye Kang Takahiro Nii Naoki Isobe Yukinori Yoshimura Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens The Journal of Poultry Science histone modification innate immunity laying hen ovary salmonella enteritidis vaccine |
| title | Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens |
| title_full | Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens |
| title_fullStr | Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens |
| title_full_unstemmed | Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens |
| title_short | Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens |
| title_sort | effects of salmonella enteritidis vaccination on the expression of innate immune molecules and histone modifications in the follicular theca of laying hens |
| topic | histone modification innate immunity laying hen ovary salmonella enteritidis vaccine |
| url | https://www.jstage.jst.go.jp/article/jpsa/56/4/56_0190034/_html/-char/en |
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