Strategies for Reducing Ruminant Methane Emissions

The paper studies the effect of additional administration of ultrafine particles on the cattle rumen microbiome composition. The in vitro method was used using the ANKOM Daisy II incubator according to a specialized method. Microflora analysis was performed using MiSeq (Illumina, USA) by a new gener...

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Main Authors: Sheida E. V., Miroshnikov S. A., Duskaev G. K., Atlanderova K.N., Grechkina V.V.
Format: Article
Language:English
Published: EDP Sciences 2022-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2022/01/bioconf_sdge2022_01014.pdf
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author Sheida E. V.
Miroshnikov S. A.
Duskaev G. K.
Atlanderova K.N.
Grechkina V.V.
author_facet Sheida E. V.
Miroshnikov S. A.
Duskaev G. K.
Atlanderova K.N.
Grechkina V.V.
author_sort Sheida E. V.
collection DOAJ
description The paper studies the effect of additional administration of ultrafine particles on the cattle rumen microbiome composition. The in vitro method was used using the ANKOM Daisy II incubator according to a specialized method. Microflora analysis was performed using MiSeq (Illumina, USA) by a new generation sequencing method with a MiSeq reagent kit. After a detailed analysis of the structure and composition of the microbial community in the contents of the rumen sampled for different diets, it was found that no significant differences were observed in the bacterial communities, with the exception of a slight shift in the Bacteroidetes/Firmicutes ratio. However, we observed numerical differences in the abundance of some representatives, namely, with additional inclusion of Fe and Cr2O3, decrease in the abundance of the methane-forming species Methanobrevibacter, Methanobacterium, Methanosphaera, and Methnaomicrobium was noted regarding the control.
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spelling doaj.art-03bdf9c2b6ae4b2c8dba043bbd29ce5f2022-12-21T17:24:31ZengEDP SciencesBIO Web of Conferences2117-44582022-01-01420101410.1051/bioconf/20224201014bioconf_sdge2022_01014Strategies for Reducing Ruminant Methane EmissionsSheida E. V.0Miroshnikov S. A.1Duskaev G. K.2Atlanderova K.N.3Grechkina V.V.4Federal Research Centre of Biological Systems and Agrotechnologies, RASFederal Research Centre of Biological Systems and Agrotechnologies, RASFederal Research Centre of Biological Systems and Agrotechnologies, RASFederal Research Centre of Biological Systems and Agrotechnologies, RASFederal Research Centre of Biological Systems and Agrotechnologies, RASThe paper studies the effect of additional administration of ultrafine particles on the cattle rumen microbiome composition. The in vitro method was used using the ANKOM Daisy II incubator according to a specialized method. Microflora analysis was performed using MiSeq (Illumina, USA) by a new generation sequencing method with a MiSeq reagent kit. After a detailed analysis of the structure and composition of the microbial community in the contents of the rumen sampled for different diets, it was found that no significant differences were observed in the bacterial communities, with the exception of a slight shift in the Bacteroidetes/Firmicutes ratio. However, we observed numerical differences in the abundance of some representatives, namely, with additional inclusion of Fe and Cr2O3, decrease in the abundance of the methane-forming species Methanobrevibacter, Methanobacterium, Methanosphaera, and Methnaomicrobium was noted regarding the control.https://www.bio-conferences.org/articles/bioconf/pdf/2022/01/bioconf_sdge2022_01014.pdf
spellingShingle Sheida E. V.
Miroshnikov S. A.
Duskaev G. K.
Atlanderova K.N.
Grechkina V.V.
Strategies for Reducing Ruminant Methane Emissions
BIO Web of Conferences
title Strategies for Reducing Ruminant Methane Emissions
title_full Strategies for Reducing Ruminant Methane Emissions
title_fullStr Strategies for Reducing Ruminant Methane Emissions
title_full_unstemmed Strategies for Reducing Ruminant Methane Emissions
title_short Strategies for Reducing Ruminant Methane Emissions
title_sort strategies for reducing ruminant methane emissions
url https://www.bio-conferences.org/articles/bioconf/pdf/2022/01/bioconf_sdge2022_01014.pdf
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AT miroshnikovsa strategiesforreducingruminantmethaneemissions
AT duskaevgk strategiesforreducingruminantmethaneemissions
AT atlanderovakn strategiesforreducingruminantmethaneemissions
AT grechkinavv strategiesforreducingruminantmethaneemissions