A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice
Soy glycinin derived octapeptide (SGP8) is a peptide obtained from degradation of the soy glycinin, whose amino acid sequence is IAVPGEVA. To determine the effect of SGP8 on non-alcoholic fatty liver disease (NAFLD), steatosis HepG2 cells were induced by 1 mmol/L free fatty acid (FFA) and C57BL/6J m...
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| Format: | Article |
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000957 |
| _version_ | 1797727533111705600 |
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| author | Peng Ma Rongrong Huang Yu Ou |
| author_facet | Peng Ma Rongrong Huang Yu Ou |
| author_sort | Peng Ma |
| collection | DOAJ |
| description | Soy glycinin derived octapeptide (SGP8) is a peptide obtained from degradation of the soy glycinin, whose amino acid sequence is IAVPGEVA. To determine the effect of SGP8 on non-alcoholic fatty liver disease (NAFLD), steatosis HepG2 cells were induced by 1 mmol/L free fatty acid (FFA) and C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for 3 weeks to establish NAFLD model. The results of oil red O staining and total cholesterol (TC)/triglyceride (TG) contents showed that SGP8 could significantly reduce the lipid content of steatosis HepG2 cells. In vivo, SGP8 lowered plasma alanine aminotransferase (ALT) and low density lipoprotein (LDL) content, normalized hepatic superoxide dismutase (SOD) and malondialdehyde (MDA) production, and reduced the severity of liver inflammation. The results of Western blotting showed that SGP8 increased expression of Sirtuin-1 (SIRT1) and phosphorylation level of AMP activated protein kinase (AMPK) in hepatocytes. Through activation of SIRT1/AMPK pathway, SGP8 downregulated the expression of sterol regulatory element binding protein 1c (SREBP-1c) and its target genes ACC and FAS expression levels, and increased the phosphorylation level of acetyl CoA carboxylase (ACC). Furthermore, SGP8 also upregulated the expression of transcription factor peroxisome proliferator activated receptor α (PPARα), which was regulated by SIRT1/AMPK pathway, and its target gene CPT1 level. In conclusion, SGP8 might improve NAFLD by activating the SIRT1/AMPK pathway. Our data suggest that SGP8 may act as a novel and potent therapeutic agent against NAFLD. |
| first_indexed | 2024-03-12T11:01:06Z |
| format | Article |
| id | doaj.art-03bfce5cfeb64c29baadfd4a6fe61940 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T11:01:06Z |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj.art-03bfce5cfeb64c29baadfd4a6fe619402023-09-02T05:34:13ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111615441554A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in micePeng Ma0Rongrong Huang1Yu Ou2School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, ChinaCorresponding author at: School of Life Science and Technology, China Pharmaceutical University.; School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, ChinaSoy glycinin derived octapeptide (SGP8) is a peptide obtained from degradation of the soy glycinin, whose amino acid sequence is IAVPGEVA. To determine the effect of SGP8 on non-alcoholic fatty liver disease (NAFLD), steatosis HepG2 cells were induced by 1 mmol/L free fatty acid (FFA) and C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for 3 weeks to establish NAFLD model. The results of oil red O staining and total cholesterol (TC)/triglyceride (TG) contents showed that SGP8 could significantly reduce the lipid content of steatosis HepG2 cells. In vivo, SGP8 lowered plasma alanine aminotransferase (ALT) and low density lipoprotein (LDL) content, normalized hepatic superoxide dismutase (SOD) and malondialdehyde (MDA) production, and reduced the severity of liver inflammation. The results of Western blotting showed that SGP8 increased expression of Sirtuin-1 (SIRT1) and phosphorylation level of AMP activated protein kinase (AMPK) in hepatocytes. Through activation of SIRT1/AMPK pathway, SGP8 downregulated the expression of sterol regulatory element binding protein 1c (SREBP-1c) and its target genes ACC and FAS expression levels, and increased the phosphorylation level of acetyl CoA carboxylase (ACC). Furthermore, SGP8 also upregulated the expression of transcription factor peroxisome proliferator activated receptor α (PPARα), which was regulated by SIRT1/AMPK pathway, and its target gene CPT1 level. In conclusion, SGP8 might improve NAFLD by activating the SIRT1/AMPK pathway. Our data suggest that SGP8 may act as a novel and potent therapeutic agent against NAFLD.http://www.sciencedirect.com/science/article/pii/S2213453022000957Soy glycinin derived octapeptide (SGP8)Non-alcoholic fatty liver disease (NAFLD)HepG2 cellsMethionine-choline deficient (MCD)SIRT1/AMPK pathway |
| spellingShingle | Peng Ma Rongrong Huang Yu Ou A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice Food Science and Human Wellness Soy glycinin derived octapeptide (SGP8) Non-alcoholic fatty liver disease (NAFLD) HepG2 cells Methionine-choline deficient (MCD) SIRT1/AMPK pathway |
| title | A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice |
| title_full | A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice |
| title_fullStr | A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice |
| title_full_unstemmed | A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice |
| title_short | A soy glycinin derived octapeptide protects against MCD diet induced non-alcoholic fatty liver disease in mice |
| title_sort | soy glycinin derived octapeptide protects against mcd diet induced non alcoholic fatty liver disease in mice |
| topic | Soy glycinin derived octapeptide (SGP8) Non-alcoholic fatty liver disease (NAFLD) HepG2 cells Methionine-choline deficient (MCD) SIRT1/AMPK pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000957 |
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