Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species
Bacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (<i>Podoviridae</i> morphotype) and nACB2 (<i>Myoviridae</i> morphotype), which infect <i>Acinetobacter beijerinckii</i> and <i>Acinetobacter haloto...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/3/643 |
| _version_ | 1827747245314801664 |
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| author | Marta Gomes Rita Domingues Dann Turner Hugo Oliveira |
| author_facet | Marta Gomes Rita Domingues Dann Turner Hugo Oliveira |
| author_sort | Marta Gomes |
| collection | DOAJ |
| description | Bacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (<i>Podoviridae</i> morphotype) and nACB2 (<i>Myoviridae</i> morphotype), which infect <i>Acinetobacter beijerinckii</i> and <i>Acinetobacter halotolerans</i>, respectively, were isolated from sewage samples. The genome sequences of nACB1 and nACB2 revealed that their genome sizes were 80,310 bp and 136,560 bp, respectively. Comparative analysis showed that both genomes are novel members of the <i>Schitoviridae</i> and the <i>Ackermannviridae</i> families, sharing ≤ 40% overall nucleotide identities with any other phages. Interestingly, among other genetic features, nACB1 encoded a very large RNA polymerase, while nACB2 displayed three putative depolymerases (two capsular depolymerases and one capsular esterase) encoded in tandem. This is the first report of phages infecting <i>A. halotolerans</i> and <i>beijerinckii</i> human pathogenic species. The findings regarding these two phages will allow us to further explore phage—<i>Acinetobacter</i> interactions and the genetic evolution for this group of phages. |
| first_indexed | 2024-03-11T05:46:24Z |
| format | Article |
| id | doaj.art-03c3085ce14b4f8c9e4f384e873949d3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T05:46:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-03c3085ce14b4f8c9e4f384e873949d32023-11-17T14:22:18ZengMDPI AGViruses1999-49152023-02-0115364310.3390/v15030643Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> SpeciesMarta Gomes0Rita Domingues1Dann Turner2Hugo Oliveira3Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCentre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalSchool of Applied Sciences, College of Health, Science and Society, University of the West of England, Bristol Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, UKCentre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalBacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (<i>Podoviridae</i> morphotype) and nACB2 (<i>Myoviridae</i> morphotype), which infect <i>Acinetobacter beijerinckii</i> and <i>Acinetobacter halotolerans</i>, respectively, were isolated from sewage samples. The genome sequences of nACB1 and nACB2 revealed that their genome sizes were 80,310 bp and 136,560 bp, respectively. Comparative analysis showed that both genomes are novel members of the <i>Schitoviridae</i> and the <i>Ackermannviridae</i> families, sharing ≤ 40% overall nucleotide identities with any other phages. Interestingly, among other genetic features, nACB1 encoded a very large RNA polymerase, while nACB2 displayed three putative depolymerases (two capsular depolymerases and one capsular esterase) encoded in tandem. This is the first report of phages infecting <i>A. halotolerans</i> and <i>beijerinckii</i> human pathogenic species. The findings regarding these two phages will allow us to further explore phage—<i>Acinetobacter</i> interactions and the genetic evolution for this group of phages.https://www.mdpi.com/1999-4915/15/3/643<i>Acinetobacter</i>bacteriophagescomparative genomicsbioinformatics |
| spellingShingle | Marta Gomes Rita Domingues Dann Turner Hugo Oliveira Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species Viruses <i>Acinetobacter</i> bacteriophages comparative genomics bioinformatics |
| title | Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species |
| title_full | Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species |
| title_fullStr | Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species |
| title_full_unstemmed | Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species |
| title_short | Genomic Analysis of Two Novel Bacteriophages Infecting <i>Acinetobacter beijerinckii</i> and <i>halotolerans</i> Species |
| title_sort | genomic analysis of two novel bacteriophages infecting i acinetobacter beijerinckii i and i halotolerans i species |
| topic | <i>Acinetobacter</i> bacteriophages comparative genomics bioinformatics |
| url | https://www.mdpi.com/1999-4915/15/3/643 |
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