Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection
Polymerase chain reaction (PCR) has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand,...
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MDPI AG
2017-02-01
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| Series: | Inventions |
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| Online Access: | http://www.mdpi.com/2411-5134/2/1/3 |
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| author | Wen-Pin Chou Chien Lee Zong-Jyun Hsu Mei-Hui Lai Long-Sheng Kuo Ping-Hei Chen |
| author_facet | Wen-Pin Chou Chien Lee Zong-Jyun Hsu Mei-Hui Lai Long-Sheng Kuo Ping-Hei Chen |
| author_sort | Wen-Pin Chou |
| collection | DOAJ |
| description | Polymerase chain reaction (PCR) has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand, partial heating on the various locations of vessels to induce convective solution flows by buoyancy have been used for DNA amplification in recent years. In this research, we develop a new convective PCR platform, capillary loop convective polymerase chain reaction (clcPCR), which can generate one direction flow and make the PCR reaction more stable. The U-shaped loop capillaries with 1.6 mm inner diameter are designed as PCR reagent containers. The clcPCR platform utilizes one isothermal heater for heating the bottom of the loop capillary and a CCD device for detecting real-time amplifying fluorescence signals. The stable flow was generated in the U-shaped container and the amplification process could be finished in 25 min. Our experiments with different initial concentrations of DNA templates demonstrate that clcPCR can be applied for precise quantification. Multiple sample testing and real-time quantification will be achieved in future studies. |
| first_indexed | 2024-04-12T23:13:28Z |
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| id | doaj.art-03c6434c93de4dd2a04832fb93cd4203 |
| institution | Directory Open Access Journal |
| issn | 2411-5134 |
| language | English |
| last_indexed | 2024-04-12T23:13:28Z |
| publishDate | 2017-02-01 |
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| series | Inventions |
| spelling | doaj.art-03c6434c93de4dd2a04832fb93cd42032022-12-22T03:12:44ZengMDPI AGInventions2411-51342017-02-0121310.3390/inventions2010003inventions2010003Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence DetectionWen-Pin Chou0Chien Lee1Zong-Jyun Hsu2Mei-Hui Lai3Long-Sheng Kuo4Ping-Hei Chen5Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 10617, TaiwanPolymerase chain reaction (PCR) has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand, partial heating on the various locations of vessels to induce convective solution flows by buoyancy have been used for DNA amplification in recent years. In this research, we develop a new convective PCR platform, capillary loop convective polymerase chain reaction (clcPCR), which can generate one direction flow and make the PCR reaction more stable. The U-shaped loop capillaries with 1.6 mm inner diameter are designed as PCR reagent containers. The clcPCR platform utilizes one isothermal heater for heating the bottom of the loop capillary and a CCD device for detecting real-time amplifying fluorescence signals. The stable flow was generated in the U-shaped container and the amplification process could be finished in 25 min. Our experiments with different initial concentrations of DNA templates demonstrate that clcPCR can be applied for precise quantification. Multiple sample testing and real-time quantification will be achieved in future studies.http://www.mdpi.com/2411-5134/2/1/3convective polymerase chain reactioncapillary loop convective polymerase chain reactionfluorescence detection |
| spellingShingle | Wen-Pin Chou Chien Lee Zong-Jyun Hsu Mei-Hui Lai Long-Sheng Kuo Ping-Hei Chen Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection Inventions convective polymerase chain reaction capillary loop convective polymerase chain reaction fluorescence detection |
| title | Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection |
| title_full | Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection |
| title_fullStr | Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection |
| title_full_unstemmed | Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection |
| title_short | Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection |
| title_sort | development of capillary loop convective polymerase chain reaction platform with real time fluorescence detection |
| topic | convective polymerase chain reaction capillary loop convective polymerase chain reaction fluorescence detection |
| url | http://www.mdpi.com/2411-5134/2/1/3 |
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