IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin
Macrophages can be polarized into two subsets: a proinflammatory (M1) or an anti-inflammatory (M2) phenotype. In this study, we show that an increased M1-to-M2 ratio associated with a decrease in IL-34 induces skin inflammaging. The total number of macrophages in the dermis did not change, but the n...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | JID Innovations |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667026722000194 |
| _version_ | 1818211735920705536 |
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| author | Satoshi Horiba Ryota Kami Taiki Tsutsui Junichi Hosoi |
| author_facet | Satoshi Horiba Ryota Kami Taiki Tsutsui Junichi Hosoi |
| author_sort | Satoshi Horiba |
| collection | DOAJ |
| description | Macrophages can be polarized into two subsets: a proinflammatory (M1) or an anti-inflammatory (M2) phenotype. In this study, we show that an increased M1-to-M2 ratio associated with a decrease in IL-34 induces skin inflammaging. The total number of macrophages in the dermis did not change, but the number of M2 macrophages was significantly decreased. Thus, the M1-to-M2 ratio was significantly increased in sun-exposed aged skin and positively correlated with the percentage of p21+ and p16+ senescent cells in the dermis. The supernatant of M1 macrophages increased the percentages of senescence-associated β-galactosidase‒positive cells, whereas the supernatant of M2 macrophages decreased the percentages of senescence-associated β-galactosidase‒positive cells in vitro. Among the mechanisms that could explain the increase in the M1-to-M2 ratio, we found that the number of IL-34+ cells was decreased in aged skin and negatively correlated with the M1-to-M2 ratio. Furthermore, IL-34 induced the expression of CD206 and IL-10, which are M2 macrophage markers, in an in vitro assay. Our results suggest that a reduction in epidermal IL-34 in aged skin may skew the M1/M2 balance in the dermis and lead to low-grade chronic inflammation and inflammaging. |
| first_indexed | 2024-12-12T05:37:14Z |
| format | Article |
| id | doaj.art-03c6cb36e4c84f308f88a6d18ce71f3d |
| institution | Directory Open Access Journal |
| issn | 2667-0267 |
| language | English |
| last_indexed | 2024-12-12T05:37:14Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JID Innovations |
| spelling | doaj.art-03c6cb36e4c84f308f88a6d18ce71f3d2022-12-22T00:36:05ZengElsevierJID Innovations2667-02672022-05-0123100112IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human SkinSatoshi Horiba0Ryota Kami1Taiki Tsutsui2Junichi Hosoi3Shiseido Global Innovation Center, Yokohama, Japan; Correspondence: Satoshi Horiba, Shiseido Global Innovation Center, 1-2-11, Takashima, Nishi-ku, Yokohama 220-0011, Japan.Shiseido Global Innovation Center, Yokohama, JapanShiseido Global Innovation Center, Yokohama, JapanShiseido Global Innovation Center, Yokohama, JapanMacrophages can be polarized into two subsets: a proinflammatory (M1) or an anti-inflammatory (M2) phenotype. In this study, we show that an increased M1-to-M2 ratio associated with a decrease in IL-34 induces skin inflammaging. The total number of macrophages in the dermis did not change, but the number of M2 macrophages was significantly decreased. Thus, the M1-to-M2 ratio was significantly increased in sun-exposed aged skin and positively correlated with the percentage of p21+ and p16+ senescent cells in the dermis. The supernatant of M1 macrophages increased the percentages of senescence-associated β-galactosidase‒positive cells, whereas the supernatant of M2 macrophages decreased the percentages of senescence-associated β-galactosidase‒positive cells in vitro. Among the mechanisms that could explain the increase in the M1-to-M2 ratio, we found that the number of IL-34+ cells was decreased in aged skin and negatively correlated with the M1-to-M2 ratio. Furthermore, IL-34 induced the expression of CD206 and IL-10, which are M2 macrophage markers, in an in vitro assay. Our results suggest that a reduction in epidermal IL-34 in aged skin may skew the M1/M2 balance in the dermis and lead to low-grade chronic inflammation and inflammaging.http://www.sciencedirect.com/science/article/pii/S2667026722000194 |
| spellingShingle | Satoshi Horiba Ryota Kami Taiki Tsutsui Junichi Hosoi IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin JID Innovations |
| title | IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin |
| title_full | IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin |
| title_fullStr | IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin |
| title_full_unstemmed | IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin |
| title_short | IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin |
| title_sort | il 34 downregulation associated m1 m2 macrophage imbalance is related to inflammaging in sun exposed human skin |
| url | http://www.sciencedirect.com/science/article/pii/S2667026722000194 |
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