How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets
We have utilized patients' own oral mucosa as a cell source for the fabrication of transplantable epithelial cell sheets to treat limbal stem cell deficiency and mucosal defects after endoscopic submucosal dissection of esophageal cancer. Because there are abundant microbiotas in the human oral...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320415000061 |
| _version_ | 1818909487313977344 |
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| author | Ryo Takagi Shinichiro Kobayashi Masayuki Yamato Toshiyuki Owaki Yoshiyuki Kasai Takahiro Hosoi Yusuke Sakai Kengo Kanetaka Tokutaro Minamizato Asuka Minematsu Makoto Kondo Nobuo Kanai Naoyuki Yamaguchi Kazuhiro Nagai Yasushi Miyazaki Naoya Takeda Fumio Fukai Izumi Asahina Taiga Miyazaki Shigeru Kohno Masakazu Yamamoto Kazuhiko Nakao Susumu Eguchi Teruo Okano |
| author_facet | Ryo Takagi Shinichiro Kobayashi Masayuki Yamato Toshiyuki Owaki Yoshiyuki Kasai Takahiro Hosoi Yusuke Sakai Kengo Kanetaka Tokutaro Minamizato Asuka Minematsu Makoto Kondo Nobuo Kanai Naoyuki Yamaguchi Kazuhiro Nagai Yasushi Miyazaki Naoya Takeda Fumio Fukai Izumi Asahina Taiga Miyazaki Shigeru Kohno Masakazu Yamamoto Kazuhiko Nakao Susumu Eguchi Teruo Okano |
| author_sort | Ryo Takagi |
| collection | DOAJ |
| description | We have utilized patients' own oral mucosa as a cell source for the fabrication of transplantable epithelial cell sheets to treat limbal stem cell deficiency and mucosal defects after endoscopic submucosal dissection of esophageal cancer. Because there are abundant microbiotas in the human oral cavity, the oral mucosa was sterilized and 40 μg/mL gentamicin and 0.27 μg/mL amphotericin B were added to the culture medium in our protocol. Although an oral surgeon carefully checked each patient's oral cavity and although candidiasis was not observed before taking the biopsy, contamination with Candida albicans (C. albicans) was detected in the conditioned medium during cell sheet fabrication. After adding 1 μg/mL amphotericin B to the transportation medium during transport from Nagasaki University Hospital to Tokyo Women's Medical University, which are 1200 km apart, no proliferation of C. albicans was observed. These results indicated that the supplementation of transportation medium with antimycotics would be useful for preventing contamination with C. albicans derived from the oral mucosa without hampering cell proliferation. |
| first_indexed | 2024-12-19T22:27:41Z |
| format | Article |
| id | doaj.art-03c6cc028cbe4af0acbe5b567ce75cdd |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-19T22:27:41Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-03c6cc028cbe4af0acbe5b567ce75cdd2022-12-21T20:03:27ZengElsevierRegenerative Therapy2352-32042015-06-011C1410.1016/j.reth.2014.12.002How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheetsRyo Takagi0Shinichiro Kobayashi1Masayuki Yamato2Toshiyuki Owaki3Yoshiyuki Kasai4Takahiro Hosoi5Yusuke Sakai6Kengo Kanetaka7Tokutaro Minamizato8Asuka Minematsu9Makoto Kondo10Nobuo Kanai11Naoyuki Yamaguchi12Kazuhiro Nagai13Yasushi Miyazaki14Naoya Takeda15Fumio Fukai16Izumi Asahina17Taiga Miyazaki18Shigeru Kohno19Masakazu Yamamoto20Kazuhiko Nakao21Susumu Eguchi22Teruo Okano23Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Surgery, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanDepartment of Surgery, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanDepartment of Regenerative Oral Surgery, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Science, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Gastroenterology and Hepatology, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanTransfusion and Cell Therapy Unit, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanTransfusion and Cell Therapy Unit, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanGraduate School of Advanced Science and Engineering, Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-Cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Pharmaceutical Science, Graduate School of Pharmaceutical Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba 278-8510, JapanDepartment of Regenerative Oral Surgery, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Science, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanDepartment of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Science, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8501, JapanDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Gastroenterology and Hepatology, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanDepartment of Surgery, Graduate School of Biomedical Science, Nagasaki University, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanWe have utilized patients' own oral mucosa as a cell source for the fabrication of transplantable epithelial cell sheets to treat limbal stem cell deficiency and mucosal defects after endoscopic submucosal dissection of esophageal cancer. Because there are abundant microbiotas in the human oral cavity, the oral mucosa was sterilized and 40 μg/mL gentamicin and 0.27 μg/mL amphotericin B were added to the culture medium in our protocol. Although an oral surgeon carefully checked each patient's oral cavity and although candidiasis was not observed before taking the biopsy, contamination with Candida albicans (C. albicans) was detected in the conditioned medium during cell sheet fabrication. After adding 1 μg/mL amphotericin B to the transportation medium during transport from Nagasaki University Hospital to Tokyo Women's Medical University, which are 1200 km apart, no proliferation of C. albicans was observed. These results indicated that the supplementation of transportation medium with antimycotics would be useful for preventing contamination with C. albicans derived from the oral mucosa without hampering cell proliferation.http://www.sciencedirect.com/science/article/pii/S2352320415000061Amphotericin BCandida albicansOral mucosal epithelial cell |
| spellingShingle | Ryo Takagi Shinichiro Kobayashi Masayuki Yamato Toshiyuki Owaki Yoshiyuki Kasai Takahiro Hosoi Yusuke Sakai Kengo Kanetaka Tokutaro Minamizato Asuka Minematsu Makoto Kondo Nobuo Kanai Naoyuki Yamaguchi Kazuhiro Nagai Yasushi Miyazaki Naoya Takeda Fumio Fukai Izumi Asahina Taiga Miyazaki Shigeru Kohno Masakazu Yamamoto Kazuhiko Nakao Susumu Eguchi Teruo Okano How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets Regenerative Therapy Amphotericin B Candida albicans Oral mucosal epithelial cell |
| title | How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| title_full | How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| title_fullStr | How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| title_full_unstemmed | How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| title_short | How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| title_sort | how to prevent contamination with candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets |
| topic | Amphotericin B Candida albicans Oral mucosal epithelial cell |
| url | http://www.sciencedirect.com/science/article/pii/S2352320415000061 |
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