A Two-Step Approach for Diagnosing Glutamate Dehydrogenase Genes by Conventional Polymerase Chain Reaction from Clostridium difficile Isolates

A Two-Step Approach for Diagnosing Glutamate Dehydrogenase Genes by Conventional Polymerase Chain Reaction from Clostridium difficile Isolates

BACKGROUND Clostridium difficile is the major causative agent of nosocomial antibiotic-associated colitis. The gold standard for C. difficile detection is stool culture followed by cytotoxic assay, although it is laborious and time-consuming. We developed a screening test based on a two-step conven...

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Bibliographic Details
Main Authors: Sepideh Khodaparast, Ashraf Mohabati Mobarez, Mehdi Saberifiroozi
Format: Article
Language:English
Published: Iranian Association of Gastroenterology and Hepatology, Shiraz University of Medical Sciences 2019-07-01
Series:Middle East Journal of Digestive Diseases
Subjects:
Clostridium difficile
Colitis
Toxigenic culture
Cdd3
gluD
tcdA
Online Access:http://www.mejdd.org/index.php/mejdd/article/view/1982

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