β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis.
The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5533460?pdf=render |
| _version_ | 1818944552017330176 |
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| author | Laura Medina-Puche María José Castelló Juan Vicente Canet Julián Lamilla María Laura Colombo Pablo Tornero |
| author_facet | Laura Medina-Puche María José Castelló Juan Vicente Canet Julián Lamilla María Laura Colombo Pablo Tornero |
| author_sort | Laura Medina-Puche |
| collection | DOAJ |
| description | The plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4-βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants. |
| first_indexed | 2024-12-20T07:45:02Z |
| format | Article |
| id | doaj.art-03cb1a65278b495da0212ac7bb404008 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:45:02Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-03cb1a65278b495da0212ac7bb4040082022-12-21T19:48:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018182010.1371/journal.pone.0181820β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis.Laura Medina-PucheMaría José CastellóJuan Vicente CanetJulián LamillaMaría Laura ColomboPablo TorneroThe plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4-βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants.http://europepmc.org/articles/PMC5533460?pdf=render |
| spellingShingle | Laura Medina-Puche María José Castelló Juan Vicente Canet Julián Lamilla María Laura Colombo Pablo Tornero β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. PLoS ONE |
| title | β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. |
| title_full | β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. |
| title_fullStr | β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. |
| title_full_unstemmed | β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. |
| title_short | β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. |
| title_sort | β carbonic anhydrases play a role in salicylic acid perception in arabidopsis |
| url | http://europepmc.org/articles/PMC5533460?pdf=render |
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