Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip
Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromato...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1460325 |
| _version_ | 1818982626840543232 |
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| author | Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang |
| author_facet | Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang |
| author_sort | Min Feng |
| collection | DOAJ |
| description | Clonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO. |
| first_indexed | 2024-12-20T17:50:13Z |
| format | Article |
| id | doaj.art-03cd1538a0874461bc2ebfc49657ec8b |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-20T17:50:13Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-03cd1538a0874461bc2ebfc49657ec8b2022-12-21T19:30:51ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129182183210.1080/09540105.2018.14603251460325Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test stripMin Feng0Steven Suryoprabowo1Hong Tao2Liqiang Liu3Qiankun Zheng4Hua Kuang5Huaian Entry-Exit Inspection and Quarantine BureauState Key Laboratory of Food Science and Technology, Jiangnan UniversityNational Institute of MetrologyState Key Laboratory of Food Science and Technology, Jiangnan UniversityDelishi GroupState Key Laboratory of Food Science and Technology, Jiangnan UniversityClonidine (CLO) preferentially stimulates central α-2-adrenoceptors and causes both desirable and undesirable effects. Undesirable effects include sedation and withdrawal reactions, which occur as a sudden rise in arterial pressure, nervousness, agitation, and increased heart rate. An immunochromatographic test strip was developed for the rapid simultaneous detection of CLO and its cross-reactivity with apraclonidine (ACLO) in pig urine. The antigen CLO-OVA and goat anti-mouse IgG were attached to a nitrocellulose membrane as control line and test line. The cut-off limits of the test strips for CLO and ACLO were found to be 2.5 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. The results revealed that the developed method is a sensitive, rapid, and simple tool for the detection of CLO and ACLO.http://dx.doi.org/10.1080/09540105.2018.1460325apraclonidineclonidineimmunochromatographic assaymonoclonal antibodystrip test |
| spellingShingle | Min Feng Steven Suryoprabowo Hong Tao Liqiang Liu Qiankun Zheng Hua Kuang Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip Food and Agricultural Immunology apraclonidine clonidine immunochromatographic assay monoclonal antibody strip test |
| title | Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_full | Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_fullStr | Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_full_unstemmed | Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_short | Rapid detection of clonidine and its cross-reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| title_sort | rapid detection of clonidine and its cross reactivity with apraclonidine in pig urine using an immunochromatographic test strip |
| topic | apraclonidine clonidine immunochromatographic assay monoclonal antibody strip test |
| url | http://dx.doi.org/10.1080/09540105.2018.1460325 |
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