Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae)
Risk assessment of pesticides involves ecotoxicological testing. In case pesticide exposure to bees is likely, toxicity tests are performed with honey bees (Apis mellifera), with a tiered approach, for which validated and internationally accepted test protocols exist. However, concerns have grown re...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321002542 |
| _version_ | 1823933708152864768 |
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| author | Gregor Claus Matti Pisman Pieter Spanoghe Guy Smagghe Maxime Eeraerts |
| author_facet | Gregor Claus Matti Pisman Pieter Spanoghe Guy Smagghe Maxime Eeraerts |
| author_sort | Gregor Claus |
| collection | DOAJ |
| description | Risk assessment of pesticides involves ecotoxicological testing. In case pesticide exposure to bees is likely, toxicity tests are performed with honey bees (Apis mellifera), with a tiered approach, for which validated and internationally accepted test protocols exist. However, concerns have grown regarding the protection of non-Apis bees [bumble bees (Bombus spp.), solitary and stingless bees], given their different life cycles and therefore distinct exposure routes. Larvae of solitary bees of the genus Osmia feed on unprocessed pollen during development, yet no toxicity test protocol is internationally accepted or validated to assess the impact of pesticide exposure during this stage of their life cycle. Therefore, the purpose of this study is to further validate a test protocol with two solitary bee species (O. cornuta and O. bicornis) to assess lethal and sublethal effects of pesticide exposure on larval development. Larvae were exposed to thiacloprid (neonicotinoid insecticide) mixed in a new, artificial pollen provision. Both lethal (developmental and winter mortality) and sublethal endpoints (larval development time, pollen provision consumption, cocoon weight, emergence time and adult longevity) were recorded. Effects of lower, more environmentally realistic doses were only reflected in sublethal endpoints. In both bee species, thiacloprid treatment was associated with increased developmental mortality and larval development time, and decreased pollen provision consumption and cocoon weight. The test protocol proved valid and robust and showed that for higher doses of thiacloprid the acute endpoint (larval mortality) is sufficient. In addition, new insights needed to develop a standardized test protocol were acquired, such as testing of a positive control for the first time and selection of male and female individuals at egg level. |
| first_indexed | 2024-12-16T22:09:29Z |
| format | Article |
| id | doaj.art-03d2c2a44cc94ef8b44c516f8554bec7 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-16T22:09:29Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-03d2c2a44cc94ef8b44c516f8554bec72022-12-21T22:14:21ZengElsevierEcotoxicology and Environmental Safety0147-65132021-06-01215112143Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae)Gregor Claus0Matti Pisman1Pieter Spanoghe2Guy Smagghe3Maxime Eeraerts4Laboratory of Crop Protection Chemistry, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium; Corresponding author.Laboratory of Agrozoology, Ghent University, Coupure Links 653, B-9000 Ghent, BelgiumLaboratory of Crop Protection Chemistry, Ghent University, Coupure Links 653, B-9000 Ghent, BelgiumLaboratory of Agrozoology, Ghent University, Coupure Links 653, B-9000 Ghent, BelgiumLaboratory of Agrozoology, Ghent University, Coupure Links 653, B-9000 Ghent, BelgiumRisk assessment of pesticides involves ecotoxicological testing. In case pesticide exposure to bees is likely, toxicity tests are performed with honey bees (Apis mellifera), with a tiered approach, for which validated and internationally accepted test protocols exist. However, concerns have grown regarding the protection of non-Apis bees [bumble bees (Bombus spp.), solitary and stingless bees], given their different life cycles and therefore distinct exposure routes. Larvae of solitary bees of the genus Osmia feed on unprocessed pollen during development, yet no toxicity test protocol is internationally accepted or validated to assess the impact of pesticide exposure during this stage of their life cycle. Therefore, the purpose of this study is to further validate a test protocol with two solitary bee species (O. cornuta and O. bicornis) to assess lethal and sublethal effects of pesticide exposure on larval development. Larvae were exposed to thiacloprid (neonicotinoid insecticide) mixed in a new, artificial pollen provision. Both lethal (developmental and winter mortality) and sublethal endpoints (larval development time, pollen provision consumption, cocoon weight, emergence time and adult longevity) were recorded. Effects of lower, more environmentally realistic doses were only reflected in sublethal endpoints. In both bee species, thiacloprid treatment was associated with increased developmental mortality and larval development time, and decreased pollen provision consumption and cocoon weight. The test protocol proved valid and robust and showed that for higher doses of thiacloprid the acute endpoint (larval mortality) is sufficient. In addition, new insights needed to develop a standardized test protocol were acquired, such as testing of a positive control for the first time and selection of male and female individuals at egg level.http://www.sciencedirect.com/science/article/pii/S0147651321002542Pesticide risk assessmentSolitary bee larvaeNeonicotinoidsSublethal endpointsLethal endpointsOral test protocol |
| spellingShingle | Gregor Claus Matti Pisman Pieter Spanoghe Guy Smagghe Maxime Eeraerts Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) Ecotoxicology and Environmental Safety Pesticide risk assessment Solitary bee larvae Neonicotinoids Sublethal endpoints Lethal endpoints Oral test protocol |
| title | Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) |
| title_full | Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) |
| title_fullStr | Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) |
| title_full_unstemmed | Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) |
| title_short | Larval oral exposure to thiacloprid: Dose-response toxicity testing in solitary bees, Osmia spp. (Hymenoptera: Megachilidae) |
| title_sort | larval oral exposure to thiacloprid dose response toxicity testing in solitary bees osmia spp hymenoptera megachilidae |
| topic | Pesticide risk assessment Solitary bee larvae Neonicotinoids Sublethal endpoints Lethal endpoints Oral test protocol |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321002542 |
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