Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice
Hydrogen sulfide (H2S) plays a vital role in Al3+ stress resistance in plants, but the underlying mechanism is unclear. In the present study, pretreatment with 2 μM of the H2S donor NaHS significantly alleviated the inhibition of root elongation caused by Al toxicity in rice roots, which was accompa...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00294/full |
| _version_ | 1819041372314796032 |
|---|---|
| author | Chun Q. Zhu Jun H. Zhang Li M. Sun Lian F. Zhu Buhailiqem Abliz Wen J. Hu Chu Zhong Zhi G. Bai Hussain Sajid Xiao C. Cao Qian Y. Jin |
| author_facet | Chun Q. Zhu Jun H. Zhang Li M. Sun Lian F. Zhu Buhailiqem Abliz Wen J. Hu Chu Zhong Zhi G. Bai Hussain Sajid Xiao C. Cao Qian Y. Jin |
| author_sort | Chun Q. Zhu |
| collection | DOAJ |
| description | Hydrogen sulfide (H2S) plays a vital role in Al3+ stress resistance in plants, but the underlying mechanism is unclear. In the present study, pretreatment with 2 μM of the H2S donor NaHS significantly alleviated the inhibition of root elongation caused by Al toxicity in rice roots, which was accompanied by a decrease in Al contents in root tips under 50 μM Al3+ treatment. NaHS pretreatment decreased the negative charge in cell walls by reducing the activity of pectin methylesterase and decreasing the pectin and hemicellulose contents in rice roots. This treatment also masked Al-binding sites in the cell wall by upregulating the expression of OsSATR1 and OsSTAR2 in roots and reduced Al binding in the cell wall by stimulating the expression of the citrate acid exudation gene OsFRDL4 and increasing the secretion of citrate acid. In addition, NaHS pretreatment decreased the symplasmic Al content by downregulating the expression of OsNRAT1, and increasing the translocation of cytoplasmic Al to the vacuole via upregulating the expression of OsALS1. The increment of antioxidant enzyme [superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD)] activity with NaHS pretreatment significantly decreased the MDA and H2O2 content in rice roots, thereby reducing the damage of Al3+ toxicity on membrane integrity in rice. H2S exhibits crosstalk with nitric oxide (NO) in response to Al toxicity, and through reducing NO content in root tips to alleviate Al toxicity. Together, this study establishes that H2S alleviates Al toxicity by decreasing the Al content in the apoplast and symplast of rice roots. |
| first_indexed | 2024-12-21T09:23:57Z |
| format | Article |
| id | doaj.art-03d3881f4347493ba472d39f8b6fda20 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T09:23:57Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-03d3881f4347493ba472d39f8b6fda202022-12-21T19:08:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00294339264Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in RiceChun Q. Zhu0Jun H. Zhang1Li M. Sun2Lian F. Zhu3Buhailiqem Abliz4Wen J. Hu5Chu Zhong6Zhi G. Bai7Hussain Sajid8Xiao C. Cao9Qian Y. Jin10State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Soil and Sustainable Agriculture, China Institute of Soil Science, Chinese Academy of Sciences, Nanjing, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaNuclear Technology Biotechnology Research Institute, Xinjiang Academy of Agricultural Sciences, Ürümqi, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, ChinaHydrogen sulfide (H2S) plays a vital role in Al3+ stress resistance in plants, but the underlying mechanism is unclear. In the present study, pretreatment with 2 μM of the H2S donor NaHS significantly alleviated the inhibition of root elongation caused by Al toxicity in rice roots, which was accompanied by a decrease in Al contents in root tips under 50 μM Al3+ treatment. NaHS pretreatment decreased the negative charge in cell walls by reducing the activity of pectin methylesterase and decreasing the pectin and hemicellulose contents in rice roots. This treatment also masked Al-binding sites in the cell wall by upregulating the expression of OsSATR1 and OsSTAR2 in roots and reduced Al binding in the cell wall by stimulating the expression of the citrate acid exudation gene OsFRDL4 and increasing the secretion of citrate acid. In addition, NaHS pretreatment decreased the symplasmic Al content by downregulating the expression of OsNRAT1, and increasing the translocation of cytoplasmic Al to the vacuole via upregulating the expression of OsALS1. The increment of antioxidant enzyme [superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD)] activity with NaHS pretreatment significantly decreased the MDA and H2O2 content in rice roots, thereby reducing the damage of Al3+ toxicity on membrane integrity in rice. H2S exhibits crosstalk with nitric oxide (NO) in response to Al toxicity, and through reducing NO content in root tips to alleviate Al toxicity. Together, this study establishes that H2S alleviates Al toxicity by decreasing the Al content in the apoplast and symplast of rice roots.http://journal.frontiersin.org/article/10.3389/fpls.2018.00294/fullaluminum toxicityhydrogen sulfidecell wallroot elongationgene expressionrice |
| spellingShingle | Chun Q. Zhu Jun H. Zhang Li M. Sun Lian F. Zhu Buhailiqem Abliz Wen J. Hu Chu Zhong Zhi G. Bai Hussain Sajid Xiao C. Cao Qian Y. Jin Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice Frontiers in Plant Science aluminum toxicity hydrogen sulfide cell wall root elongation gene expression rice |
| title | Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice |
| title_full | Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice |
| title_fullStr | Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice |
| title_full_unstemmed | Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice |
| title_short | Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice |
| title_sort | hydrogen sulfide alleviates aluminum toxicity via decreasing apoplast and symplast al contents in rice |
| topic | aluminum toxicity hydrogen sulfide cell wall root elongation gene expression rice |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00294/full |
| work_keys_str_mv | AT chunqzhu hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT junhzhang hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT limsun hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT lianfzhu hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT buhailiqemabliz hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT wenjhu hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT chuzhong hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT zhigbai hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT hussainsajid hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT xiaoccao hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice AT qianyjin hydrogensulfidealleviatesaluminumtoxicityviadecreasingapoplastandsymplastalcontentsinrice |