Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage
Evolution of RNA bacteriophages of the family <i>Leviviridae</i> is governed by the high error rates of their RNA-dependent RNA polymerases. This fact, together with their large population sizes, leads to the generation of highly heterogeneous populations that adapt rapidly to most chang...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6815 |
| _version_ | 1797528849614897152 |
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| author | María Arribas Ester Lázaro |
| author_facet | María Arribas Ester Lázaro |
| author_sort | María Arribas |
| collection | DOAJ |
| description | Evolution of RNA bacteriophages of the family <i>Leviviridae</i> is governed by the high error rates of their RNA-dependent RNA polymerases. This fact, together with their large population sizes, leads to the generation of highly heterogeneous populations that adapt rapidly to most changes in the environment. Throughout adaptation, the different mutants that make up a viral population compete with each other in a non-trivial process in which their selective values change over time due to the generation of new mutations. In this work we have characterised the intra-population dynamics of a well-studied levivirus, Qβ, when it is propagated at a higher-than-optimal temperature. Our results show that adapting populations experienced rapid changes that involved the ascent of particular genotypes and the loss of some beneficial mutations of early generation. Artificially reconstructed populations, containing a fraction of the diversity present in actual populations, fixed mutations more rapidly, illustrating how population bottlenecks may guide the adaptive pathways. The conclusion is that, when the availability of beneficial mutations under a particular selective condition is elevated, the final outcome of adaptation depends more on the occasional occurrence of population bottlenecks and how mutations combine in genomes than on the selective value of particular mutations. |
| first_indexed | 2024-03-10T10:04:11Z |
| format | Article |
| id | doaj.art-03d78bae75e44bb087fffaad68367a20 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:04:11Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-03d78bae75e44bb087fffaad68367a202023-11-22T01:38:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213681510.3390/ijms22136815Intra-Population Competition during Adaptation to Increased Temperature in an RNA BacteriophageMaría Arribas0Ester Lázaro1Department of Molecular Evolution, Centro de Astrobiología (CSIC), Instituto Nacional de Técnica Aeroespacial (INTA), Carretera de Ajalvir, Km 4, Torrejón de Ardoz, 28850 Madrid, SpainDepartment of Molecular Evolution, Centro de Astrobiología (CSIC), Instituto Nacional de Técnica Aeroespacial (INTA), Carretera de Ajalvir, Km 4, Torrejón de Ardoz, 28850 Madrid, SpainEvolution of RNA bacteriophages of the family <i>Leviviridae</i> is governed by the high error rates of their RNA-dependent RNA polymerases. This fact, together with their large population sizes, leads to the generation of highly heterogeneous populations that adapt rapidly to most changes in the environment. Throughout adaptation, the different mutants that make up a viral population compete with each other in a non-trivial process in which their selective values change over time due to the generation of new mutations. In this work we have characterised the intra-population dynamics of a well-studied levivirus, Qβ, when it is propagated at a higher-than-optimal temperature. Our results show that adapting populations experienced rapid changes that involved the ascent of particular genotypes and the loss of some beneficial mutations of early generation. Artificially reconstructed populations, containing a fraction of the diversity present in actual populations, fixed mutations more rapidly, illustrating how population bottlenecks may guide the adaptive pathways. The conclusion is that, when the availability of beneficial mutations under a particular selective condition is elevated, the final outcome of adaptation depends more on the occasional occurrence of population bottlenecks and how mutations combine in genomes than on the selective value of particular mutations.https://www.mdpi.com/1422-0067/22/13/6815adaptationRNA virusesbacteriophagesQβmolecular evolutionclonal interference |
| spellingShingle | María Arribas Ester Lázaro Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage International Journal of Molecular Sciences adaptation RNA viruses bacteriophages Qβ molecular evolution clonal interference |
| title | Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage |
| title_full | Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage |
| title_fullStr | Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage |
| title_full_unstemmed | Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage |
| title_short | Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage |
| title_sort | intra population competition during adaptation to increased temperature in an rna bacteriophage |
| topic | adaptation RNA viruses bacteriophages Qβ molecular evolution clonal interference |
| url | https://www.mdpi.com/1422-0067/22/13/6815 |
| work_keys_str_mv | AT mariaarribas intrapopulationcompetitionduringadaptationtoincreasedtemperatureinanrnabacteriophage AT esterlazaro intrapopulationcompetitionduringadaptationtoincreasedtemperatureinanrnabacteriophage |