Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture
Membrane-based gas separation is an important unit operation in chemical industries due to its simplicity, ease of operation, reduced energy consumption, and compact structure. For gas separation, novel studies were carried out by synthesising enzyme-stabilised systems consisting of emulsion-based s...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Biology and Life Sciences Forum |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-9976/20/1/9 |
| _version_ | 1797613103623438336 |
|---|---|
| author | Suchintan Mondal Bhavna Alke Aline Machado de Castro Paloma Ortiz-Albo Usman Taqui Syed Joao Crespo Carla Brazinha |
| author_facet | Suchintan Mondal Bhavna Alke Aline Machado de Castro Paloma Ortiz-Albo Usman Taqui Syed Joao Crespo Carla Brazinha |
| author_sort | Suchintan Mondal |
| collection | DOAJ |
| description | Membrane-based gas separation is an important unit operation in chemical industries due to its simplicity, ease of operation, reduced energy consumption, and compact structure. For gas separation, novel studies were carried out by synthesising enzyme-stabilised systems consisting of emulsion-based supported liquid membranes (E-SLMs) the pores of which pores were impregnated with water-in-oil (W/O) emulsions produced by direct membrane emulsification. This technique has gained attention, as it consumes low energy and is mild and suitable for sensitive enzymes. This case study involves the capture of CO<sub>2</sub> by the enzyme carbonic anhydrase (CA). The composition of the oil phase was optimised amongst various edible oils, aiming for the one with the highest CO<sub>2</sub> sorption capability. The water phase was optimised based on the stability of the CA enzyme in the aqueous phase in the presence of various surfactants and their concentrations. The optimised emulsions consisted of 2% Tween 80 (<i>w</i>/<i>w</i>) in corn oil as the continuous phase and 0.5 g L<sup>−1</sup> CA enzyme with 5% PEG300 (<i>w</i>/<i>w</i>) in aqueous solution as the dispersed phase. The emulsions were prepared with a Microdyn Nadir UP150 polymeric membrane. These emulsions were impregnated onto a hydrophobic PVDF membrane to prepare E-SLM. For comparative studies, liquid membranes were also prepared without the CA enzyme in the emulsions, and a supported liquid membrane (SLM) was prepared by impregnating corn oil onto the membrane. Lastly, the permeabilities of the main components of biogas, CO<sub>2</sub>, and CH<sub>4</sub>, through the SLM and E-SLMs, were evaluated. The permeability of CO<sub>2</sub> increased (~15%) and CH<sub>4</sub> decreased (~60%) through the E-SLM containing CA when compared to the SLM and E-SLM without CA. Subsequently, the selectivity of CO<sub>2</sub> increased in the presence of low concentration of CA. This work suggests the enhanced, synergetic effects of carbonic anhydrase within a bio-based emulsion system for CO<sub>2</sub> capture. |
| first_indexed | 2024-03-11T06:51:12Z |
| format | Article |
| id | doaj.art-03da8cb9240a4ed9be6e9c098e957826 |
| institution | Directory Open Access Journal |
| issn | 2673-9976 |
| language | English |
| last_indexed | 2024-03-11T06:51:12Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology and Life Sciences Forum |
| spelling | doaj.art-03da8cb9240a4ed9be6e9c098e9578262023-11-17T09:55:35ZengMDPI AGBiology and Life Sciences Forum2673-99762022-11-01201910.3390/IECBM2022-13389Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> CaptureSuchintan Mondal0Bhavna Alke1Aline Machado de Castro2Paloma Ortiz-Albo3Usman Taqui Syed4Joao Crespo5Carla Brazinha6LAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalLAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalResearch and Development Center, PETROBRAS, Av. Horácio Macedo, 950 Ilha do Fundão, Rio de Janeiro 21941-915, BrazilLAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalLAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalLAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalLAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalMembrane-based gas separation is an important unit operation in chemical industries due to its simplicity, ease of operation, reduced energy consumption, and compact structure. For gas separation, novel studies were carried out by synthesising enzyme-stabilised systems consisting of emulsion-based supported liquid membranes (E-SLMs) the pores of which pores were impregnated with water-in-oil (W/O) emulsions produced by direct membrane emulsification. This technique has gained attention, as it consumes low energy and is mild and suitable for sensitive enzymes. This case study involves the capture of CO<sub>2</sub> by the enzyme carbonic anhydrase (CA). The composition of the oil phase was optimised amongst various edible oils, aiming for the one with the highest CO<sub>2</sub> sorption capability. The water phase was optimised based on the stability of the CA enzyme in the aqueous phase in the presence of various surfactants and their concentrations. The optimised emulsions consisted of 2% Tween 80 (<i>w</i>/<i>w</i>) in corn oil as the continuous phase and 0.5 g L<sup>−1</sup> CA enzyme with 5% PEG300 (<i>w</i>/<i>w</i>) in aqueous solution as the dispersed phase. The emulsions were prepared with a Microdyn Nadir UP150 polymeric membrane. These emulsions were impregnated onto a hydrophobic PVDF membrane to prepare E-SLM. For comparative studies, liquid membranes were also prepared without the CA enzyme in the emulsions, and a supported liquid membrane (SLM) was prepared by impregnating corn oil onto the membrane. Lastly, the permeabilities of the main components of biogas, CO<sub>2</sub>, and CH<sub>4</sub>, through the SLM and E-SLMs, were evaluated. The permeability of CO<sub>2</sub> increased (~15%) and CH<sub>4</sub> decreased (~60%) through the E-SLM containing CA when compared to the SLM and E-SLM without CA. Subsequently, the selectivity of CO<sub>2</sub> increased in the presence of low concentration of CA. This work suggests the enhanced, synergetic effects of carbonic anhydrase within a bio-based emulsion system for CO<sub>2</sub> capture.https://www.mdpi.com/2673-9976/20/1/9membrane emulsificationwater-in-oil emulsionsCO<sub>2</sub> capturecarbonic anhydraseenzymeemulsion-based supported liquid membrane |
| spellingShingle | Suchintan Mondal Bhavna Alke Aline Machado de Castro Paloma Ortiz-Albo Usman Taqui Syed Joao Crespo Carla Brazinha Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture Biology and Life Sciences Forum membrane emulsification water-in-oil emulsions CO<sub>2</sub> capture carbonic anhydrase enzyme emulsion-based supported liquid membrane |
| title | Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture |
| title_full | Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture |
| title_fullStr | Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture |
| title_full_unstemmed | Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture |
| title_short | Design of Enzyme Stabilization Systems for Gas Separation: Novel Studies on Formation of Enzyme Based W/O Emulsions by Direct Membrane Emulsification to Synthesise Emulsion-Based Supported Liquid Membrane for CO<sub>2</sub> Capture |
| title_sort | design of enzyme stabilization systems for gas separation novel studies on formation of enzyme based w o emulsions by direct membrane emulsification to synthesise emulsion based supported liquid membrane for co sub 2 sub capture |
| topic | membrane emulsification water-in-oil emulsions CO<sub>2</sub> capture carbonic anhydrase enzyme emulsion-based supported liquid membrane |
| url | https://www.mdpi.com/2673-9976/20/1/9 |
| work_keys_str_mv | AT suchintanmondal designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT bhavnaalke designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT alinemachadodecastro designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT palomaortizalbo designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT usmantaquisyed designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT joaocrespo designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture AT carlabrazinha designofenzymestabilizationsystemsforgasseparationnovelstudiesonformationofenzymebasedwoemulsionsbydirectmembraneemulsificationtosynthesiseemulsionbasedsupportedliquidmembraneforcosub2subcapture |