Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i>
<i>Lactobacillus brevis</i> is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of <i>L. brevis.</i> Gold nanoparticles (AuNPs) were firstly electro-deposited on the electro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/1/75 |
| Summary: | <i>Lactobacillus brevis</i> is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of <i>L. brevis.</i> Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆<sub>current</sub> and concentration of <i>L. brevis</i> from 10<sup>4</sup> CFU/mL to 10<sup>9</sup> CFU/mL. The limit of detection can be estimated to be 10<sup>3</sup> CFU/mL. |
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| ISSN: | 2072-666X |