Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i>
<i>Lactobacillus brevis</i> is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of <i>L. brevis.</i> Gold nanoparticles (AuNPs) were firstly electro-deposited on the electro...
| Үндсэн зохиолч: | |
|---|---|
| Формат: | Өгүүллэг |
| Хэл сонгох: | English |
| Хэвлэсэн: |
MDPI AG
2021-01-01
|
| Цуврал: | Micromachines |
| Нөхцлүүд: | |
| Онлайн хандалт: | https://www.mdpi.com/2072-666X/12/1/75 |
| _version_ | 1827601945858146304 |
|---|---|
| author | Le Zhao |
| author_facet | Le Zhao |
| author_sort | Le Zhao |
| collection | DOAJ |
| description | <i>Lactobacillus brevis</i> is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of <i>L. brevis.</i> Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆<sub>current</sub> and concentration of <i>L. brevis</i> from 10<sup>4</sup> CFU/mL to 10<sup>9</sup> CFU/mL. The limit of detection can be estimated to be 10<sup>3</sup> CFU/mL. |
| first_indexed | 2024-03-09T05:08:40Z |
| format | Article |
| id | doaj.art-03dac697612040a3ab49c8e3b2222ab9 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T05:08:40Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-03dac697612040a3ab49c8e3b2222ab92023-12-03T12:51:38ZengMDPI AGMicromachines2072-666X2021-01-011217510.3390/mi12010075Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i>Le Zhao0Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China<i>Lactobacillus brevis</i> is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of <i>L. brevis.</i> Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆<sub>current</sub> and concentration of <i>L. brevis</i> from 10<sup>4</sup> CFU/mL to 10<sup>9</sup> CFU/mL. The limit of detection can be estimated to be 10<sup>3</sup> CFU/mL.https://www.mdpi.com/2072-666X/12/1/75immunosensorelectrodeposition<i>Lactobacillus brevis</i>ionic liquidbeercyclic voltammetry |
| spellingShingle | Le Zhao Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> Micromachines immunosensor electrodeposition <i>Lactobacillus brevis</i> ionic liquid beer cyclic voltammetry |
| title | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> |
| title_full | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> |
| title_fullStr | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> |
| title_full_unstemmed | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> |
| title_short | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for <i>Lactobacillus brevis</i> |
| title_sort | horseradish peroxidase labelled sandwich electrochemical sensor based on ionic liquid gold nanoparticles for i lactobacillus brevis i |
| topic | immunosensor electrodeposition <i>Lactobacillus brevis</i> ionic liquid beer cyclic voltammetry |
| url | https://www.mdpi.com/2072-666X/12/1/75 |
| work_keys_str_mv | AT lezhao horseradishperoxidaselabelledsandwichelectrochemicalsensorbasedonionicliquidgoldnanoparticlesforilactobacillusbrevisi |