First report of lionfish prey from Western Florida waters as identified by DNA barcoding
DNA barcoding was used to identify prey fragments recovered from the stomachs of lionfish harvested during the 2016 Sarasota Lionfish Derby. A total of 305 prey fragments were recovered from 50 stomachs (mean = 4.6 per stomach), of which 184 (60.3%) fragments could be identified to either species or...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-09-01
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| Online Access: | https://peerj.com/articles/9922.pdf |
| _version_ | 1827607924539654144 |
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| author | Carlos A. Santamaria James Locascio Taylor M. Greenan |
| author_facet | Carlos A. Santamaria James Locascio Taylor M. Greenan |
| author_sort | Carlos A. Santamaria |
| collection | DOAJ |
| description | DNA barcoding was used to identify prey fragments recovered from the stomachs of lionfish harvested during the 2016 Sarasota Lionfish Derby. A total of 305 prey fragments were recovered from 50 stomachs (mean = 4.6 per stomach), of which 184 (60.3%) fragments could be identified to either species or genus when Cytochrome Oxidase I (COI) sequences were queried against the Barcode of Life Database. We identified 21 fish prey species which represented fourteen families and accounted for 95.7% of genetically identifiable prey items. The remaining prey items identified corresponded to six crustacean species. The four most common prey taxa in lionfish stomachs were Ptereleotris calliura (24.3%), an unidentified Microgobius species (20.4%), Diplectum formosum (14.3%), and Apogon aurolineatus (12.2%). The most frequently observed crustacean species, Metapenaeopsis goodei, was found in only three stomachs (6.1%). We also report eleven taxa as putative novel lionfish prey species, most of which are common in Florida waters. Sixteen prey items were identified as lionfish (P. volitans); however, it was not definitive whether these detections were due to cross contamination or cannibalization. This represents the first report of lionfish diets from Florida waters in the Eastern Gulf of Mexico based on barcoding efforts. Our results are largely congruent with previous COI barcoding based studies of lionfish diets, indicating these predators to be generalists exhibiting preferences for specific prey traits but with regional differences in their diets. |
| first_indexed | 2024-03-09T07:02:47Z |
| format | Article |
| id | doaj.art-03dc1690a3324c39bf7aeaab58986eb1 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:02:47Z |
| publishDate | 2020-09-01 |
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| spelling | doaj.art-03dc1690a3324c39bf7aeaab58986eb12023-12-03T09:47:15ZengPeerJ Inc.PeerJ2167-83592020-09-018e992210.7717/peerj.9922First report of lionfish prey from Western Florida waters as identified by DNA barcodingCarlos A. Santamaria0James Locascio1Taylor M. Greenan2Biology Program, Thomas University, Thomasville, GA, USAFisheries Habitat Ecology and Acoustics Program, Mote Marine Laboratory, Sarasota, FL, USABiology Program, College of Science and Mathematics, University of South Florida Sarasota-Manatee, Sarasota, FL, USADNA barcoding was used to identify prey fragments recovered from the stomachs of lionfish harvested during the 2016 Sarasota Lionfish Derby. A total of 305 prey fragments were recovered from 50 stomachs (mean = 4.6 per stomach), of which 184 (60.3%) fragments could be identified to either species or genus when Cytochrome Oxidase I (COI) sequences were queried against the Barcode of Life Database. We identified 21 fish prey species which represented fourteen families and accounted for 95.7% of genetically identifiable prey items. The remaining prey items identified corresponded to six crustacean species. The four most common prey taxa in lionfish stomachs were Ptereleotris calliura (24.3%), an unidentified Microgobius species (20.4%), Diplectum formosum (14.3%), and Apogon aurolineatus (12.2%). The most frequently observed crustacean species, Metapenaeopsis goodei, was found in only three stomachs (6.1%). We also report eleven taxa as putative novel lionfish prey species, most of which are common in Florida waters. Sixteen prey items were identified as lionfish (P. volitans); however, it was not definitive whether these detections were due to cross contamination or cannibalization. This represents the first report of lionfish diets from Florida waters in the Eastern Gulf of Mexico based on barcoding efforts. Our results are largely congruent with previous COI barcoding based studies of lionfish diets, indicating these predators to be generalists exhibiting preferences for specific prey traits but with regional differences in their diets.https://peerj.com/articles/9922.pdfPterois volitansPreyTrophic dynamicsDiet compositionFloridaCOI barcoding |
| spellingShingle | Carlos A. Santamaria James Locascio Taylor M. Greenan First report of lionfish prey from Western Florida waters as identified by DNA barcoding PeerJ Pterois volitans Prey Trophic dynamics Diet composition Florida COI barcoding |
| title | First report of lionfish prey from Western Florida waters as identified by DNA barcoding |
| title_full | First report of lionfish prey from Western Florida waters as identified by DNA barcoding |
| title_fullStr | First report of lionfish prey from Western Florida waters as identified by DNA barcoding |
| title_full_unstemmed | First report of lionfish prey from Western Florida waters as identified by DNA barcoding |
| title_short | First report of lionfish prey from Western Florida waters as identified by DNA barcoding |
| title_sort | first report of lionfish prey from western florida waters as identified by dna barcoding |
| topic | Pterois volitans Prey Trophic dynamics Diet composition Florida COI barcoding |
| url | https://peerj.com/articles/9922.pdf |
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