Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh
Background and Aim: The emergence and rapid dissemination of multidrug-resistant (MDR) bacteria in different ecosystems is a growing concern to human health, animal health, and the environment in recent years. The study aimed to determine the antibiotic resistance in Escherichia coli from deer and n...
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-10-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.12/October-2019/10.pdf |
| _version_ | 1819128536628199424 |
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| author | Md Samun Sarker Abdul Ahad Saurav Kumar Ghosh Md Shahriar Mannan Arup Sen Sirazul Islam Md Bayzid Zamila Bueaza Bupasha |
| author_facet | Md Samun Sarker Abdul Ahad Saurav Kumar Ghosh Md Shahriar Mannan Arup Sen Sirazul Islam Md Bayzid Zamila Bueaza Bupasha |
| author_sort | Md Samun Sarker |
| collection | DOAJ |
| description | Background and Aim: The emergence and rapid dissemination of multidrug-resistant (MDR) bacteria in different ecosystems is a growing concern to human health, animal health, and the environment in recent years. The study aimed to determine the antibiotic resistance in Escherichia coli from deer and nearby water sources at two different Safari parks in Bangladesh.
Materials and Methods: A number of 55 fresh fecal samples of deer and six water samples from nearby lakes were collected from two Safari parks. Samples were processed, cultured, and carried out biochemical tests for E. coli. The antibiotic susceptibility was determined by disk diffusion method. To identify the resistance genes, polymerase chain reaction was performed.
Results: A total of 32 E. coli isolates from 55 fecal samples and 6 of 6 E. coli isolates from lake water were isolated. From fecal E. coli isolates, ampicillin and sulfamethoxazole were 90.63% (n=29/32) resistant and 87.5% (n=28/32) were resistant to tetracycline and nalidixic acid. High resistance was also observed to other antibiotics. On the contrary, all E. coli isolates from water sources were 100% (n=6/6) resistant to ampicillin, tetracycline, sulfamethoxazole, and nalidixic acid. MDR was revealed in all water samples, whereas 96.88% (n=31/32) was found in fecal isolates. A number of blaTEM, tetA, and Sul2 genes were detected from both isolates.
Conclusion: This study for the 1st time highlights, a significant proportion of E. coli isolates in wildlife deer and nearby water sources were MDR in Bangladesh. |
| first_indexed | 2024-12-22T08:29:23Z |
| format | Article |
| id | doaj.art-03dddd24254148abbea488196bfa3b3c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T08:29:23Z |
| publishDate | 2019-10-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-03dddd24254148abbea488196bfa3b3c2022-12-21T18:32:33ZengVeterinary WorldVeterinary World0972-89882231-09162019-10-0112101578158310.14202/vetworld.2019.1578-1583Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in BangladeshMd Samun Sarker0Abdul Ahad1Saurav Kumar Ghosh2Md Shahriar Mannan3Arup Sen4Sirazul Islam5Md Bayzid6Zamila Bueaza Bupasha7Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Livestock Services, Upazila Livestock Office, Thakurgaon Sadar, Thakurgaon, Bangladesh.Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Livestock Services, Upazila Livestock Office, Mithamain, Kishoreganj, Bangladesh.Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Background and Aim: The emergence and rapid dissemination of multidrug-resistant (MDR) bacteria in different ecosystems is a growing concern to human health, animal health, and the environment in recent years. The study aimed to determine the antibiotic resistance in Escherichia coli from deer and nearby water sources at two different Safari parks in Bangladesh. Materials and Methods: A number of 55 fresh fecal samples of deer and six water samples from nearby lakes were collected from two Safari parks. Samples were processed, cultured, and carried out biochemical tests for E. coli. The antibiotic susceptibility was determined by disk diffusion method. To identify the resistance genes, polymerase chain reaction was performed. Results: A total of 32 E. coli isolates from 55 fecal samples and 6 of 6 E. coli isolates from lake water were isolated. From fecal E. coli isolates, ampicillin and sulfamethoxazole were 90.63% (n=29/32) resistant and 87.5% (n=28/32) were resistant to tetracycline and nalidixic acid. High resistance was also observed to other antibiotics. On the contrary, all E. coli isolates from water sources were 100% (n=6/6) resistant to ampicillin, tetracycline, sulfamethoxazole, and nalidixic acid. MDR was revealed in all water samples, whereas 96.88% (n=31/32) was found in fecal isolates. A number of blaTEM, tetA, and Sul2 genes were detected from both isolates. Conclusion: This study for the 1st time highlights, a significant proportion of E. coli isolates in wildlife deer and nearby water sources were MDR in Bangladesh.http://www.veterinaryworld.org/Vol.12/October-2019/10.pdfantibiotic-resistantdeerEscherichia colilakemultidrug-resistant |
| spellingShingle | Md Samun Sarker Abdul Ahad Saurav Kumar Ghosh Md Shahriar Mannan Arup Sen Sirazul Islam Md Bayzid Zamila Bueaza Bupasha Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh Veterinary World antibiotic-resistant deer Escherichia coli lake multidrug-resistant |
| title | Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh |
| title_full | Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh |
| title_fullStr | Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh |
| title_full_unstemmed | Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh |
| title_short | Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh |
| title_sort | antibiotic resistant escherichia coli in deer and nearby water sources at safari parks in bangladesh |
| topic | antibiotic-resistant deer Escherichia coli lake multidrug-resistant |
| url | http://www.veterinaryworld.org/Vol.12/October-2019/10.pdf |
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