Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves
he present study aimed to prepare a combined inactivated vaccine containing bovine viral diarrhea genotype-1(BVD-1),bovine viral diarrhea genotype-2 (BVD-2), infectious bovine rhinotracheitis (IBR),parainfluenza-3 (PI-3) and bovine respiratory syncytial virus(BRSV) and adjuvanted with montanide oil...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2017-06-01
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| Series: | Journal of Veterinary Medical Research |
| Subjects: | |
| Online Access: | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=516&cat_id=1 |
| _version_ | 1819104857572769792 |
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| author | Rasha,I.EL-Hawary Hanaa A. Mostafa |
| author_facet | Rasha,I.EL-Hawary Hanaa A. Mostafa |
| author_sort | Rasha,I.EL-Hawary |
| collection | DOAJ |
| description | he present study aimed to prepare a combined inactivated vaccine containing bovine viral diarrhea genotype-1(BVD-1),bovine viral diarrhea genotype-2 (BVD-2), infectious bovine rhinotracheitis (IBR),parainfluenza-3 (PI-3) and bovine respiratory syncytial virus(BRSV) and adjuvanted with montanide oil ISA 206. Quality control results proved that the pneumo-5 vaccine was pure and completely safe to be used in calves without abnormalities. Potency test was performed on two groups of calves three for each group, where the first group was vaccinated with pneumo-5 vaccine adjuvant with montanide oil ISA 206 and the second group was left as non-vaccinated control group. In group (1), serum neutralization test revealed that the serum neutralizing antibody titers in BVD-1 and BVD-2 developed more higher than the minimal acceptable titer of the protective level (log10 0.9), while log10 0.6 was protective against IBR, PI-3 and BRS viruses at one month of vaccination and remained protective till the end of experiment compared to group (2) that showed no neutralizing antibody response. The prepared vaccine proved to be highly potent as the developed BVD-1, BVD-2, IBR, PI-3 and BRSV antibodies remained within the protective level for 9 months post vaccination. |
| first_indexed | 2024-12-22T02:13:01Z |
| format | Article |
| id | doaj.art-03df8689e1184d968be1357000412c76 |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-12-22T02:13:01Z |
| publishDate | 2017-06-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-03df8689e1184d968be1357000412c762022-12-21T18:42:20ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202017-06-012414147Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calvesRasha,I.EL-Hawary0 Hanaa A. MostafaVeterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.he present study aimed to prepare a combined inactivated vaccine containing bovine viral diarrhea genotype-1(BVD-1),bovine viral diarrhea genotype-2 (BVD-2), infectious bovine rhinotracheitis (IBR),parainfluenza-3 (PI-3) and bovine respiratory syncytial virus(BRSV) and adjuvanted with montanide oil ISA 206. Quality control results proved that the pneumo-5 vaccine was pure and completely safe to be used in calves without abnormalities. Potency test was performed on two groups of calves three for each group, where the first group was vaccinated with pneumo-5 vaccine adjuvant with montanide oil ISA 206 and the second group was left as non-vaccinated control group. In group (1), serum neutralization test revealed that the serum neutralizing antibody titers in BVD-1 and BVD-2 developed more higher than the minimal acceptable titer of the protective level (log10 0.9), while log10 0.6 was protective against IBR, PI-3 and BRS viruses at one month of vaccination and remained protective till the end of experiment compared to group (2) that showed no neutralizing antibody response. The prepared vaccine proved to be highly potent as the developed BVD-1, BVD-2, IBR, PI-3 and BRSV antibodies remained within the protective level for 9 months post vaccination.http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=516&cat_id=1BVDIBRPI-3BRSVmontanide oil ISA 206 |
| spellingShingle | Rasha,I.EL-Hawary Hanaa A. Mostafa Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves Journal of Veterinary Medical Research BVD IBR PI-3 BRSV montanide oil ISA 206 |
| title | Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves |
| title_full | Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves |
| title_fullStr | Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves |
| title_full_unstemmed | Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves |
| title_short | Immunological response of locally prepared oil adjuvanted pneumo-5 vaccine in calves |
| title_sort | immunological response of locally prepared oil adjuvanted pneumo 5 vaccine in calves |
| topic | BVD IBR PI-3 BRSV montanide oil ISA 206 |
| url | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=516&cat_id=1 |
| work_keys_str_mv | AT rashaielhawary immunologicalresponseoflocallypreparedoiladjuvantedpneumo5vaccineincalves AT hanaaamostafa immunologicalresponseoflocallypreparedoiladjuvantedpneumo5vaccineincalves |