Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women
Abstract Objective To determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women. Study design Case control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Human Genomics |
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| Online Access: | https://doi.org/10.1186/s40246-021-00364-x |
| _version_ | 1811186588420407296 |
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| author | Ochuwa Adiketu Babah Oyesola Oyewole Ojewunmi Akinniyi Adediran Osuntoki Melissa A. Simon Bosede Bukola Afolabi |
| author_facet | Ochuwa Adiketu Babah Oyesola Oyewole Ojewunmi Akinniyi Adediran Osuntoki Melissa A. Simon Bosede Bukola Afolabi |
| author_sort | Ochuwa Adiketu Babah |
| collection | DOAJ |
| description | Abstract Objective To determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women. Study design Case control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-linked immunosorbent assay (ELISA) technique. Genomic DNAs were isolated from peripheral blood samples and quantified by nanodrop spectrophotometer one. Single nucleotide polymorphisms genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP). Results Mean age of participants was 32.96 ± 6.91 years for control and 32.04 ± 7.56 years for cases. VEGF levels in case and control groups were not statistically different (82.68 pg/ml [69.11–121.11 pg/ml] vs. 82.81 pg/ml [72.90–113.82 pg/ml] respectively; p = 0.967). All four genotypes examined were in Hardy–Weinberg equilibrium. Minor allele frequency of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A were 24%, 8%, 6% and 10% in the control and 19%, 9%, 5% and 14% in endometriosis patients. However, allele and genotype distributions of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A VEGF polymorphisms in endometriosis patients and control were not significantly different (p > 0.05). Conclusion Our preliminary findings revealed no association between endometriosis and − 460T > C, − 1154G > A, + 936C > T and + 2578C > A of VEGF genes among Nigerian women. |
| first_indexed | 2024-04-11T13:49:02Z |
| format | Article |
| id | doaj.art-03dfbed9dfb34bb699e65cc2183b7f98 |
| institution | Directory Open Access Journal |
| issn | 1479-7364 |
| language | English |
| last_indexed | 2024-04-11T13:49:02Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Human Genomics |
| spelling | doaj.art-03dfbed9dfb34bb699e65cc2183b7f982022-12-22T04:20:45ZengBMCHuman Genomics1479-73642021-10-0115111010.1186/s40246-021-00364-xGenetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian womenOchuwa Adiketu Babah0Oyesola Oyewole Ojewunmi1Akinniyi Adediran Osuntoki2Melissa A. Simon3Bosede Bukola Afolabi4Department of Obstetrics and Gynaecology, Faculty of Clinical Sciences, College of Medicine, University of Lagos/Lagos University Teaching HospitalDNA Laboratory, Sickle Cell Foundation NigeriaMolecular Biology Unit, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of LagosDepartment of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern UniversityDepartment of Obstetrics and Gynaecology, Faculty of Clinical Sciences, College of Medicine, University of Lagos/Lagos University Teaching HospitalAbstract Objective To determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women. Study design Case control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-linked immunosorbent assay (ELISA) technique. Genomic DNAs were isolated from peripheral blood samples and quantified by nanodrop spectrophotometer one. Single nucleotide polymorphisms genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP). Results Mean age of participants was 32.96 ± 6.91 years for control and 32.04 ± 7.56 years for cases. VEGF levels in case and control groups were not statistically different (82.68 pg/ml [69.11–121.11 pg/ml] vs. 82.81 pg/ml [72.90–113.82 pg/ml] respectively; p = 0.967). All four genotypes examined were in Hardy–Weinberg equilibrium. Minor allele frequency of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A were 24%, 8%, 6% and 10% in the control and 19%, 9%, 5% and 14% in endometriosis patients. However, allele and genotype distributions of − 460T > C, − 1154G > A, + 936C > T and + 2578C > A VEGF polymorphisms in endometriosis patients and control were not significantly different (p > 0.05). Conclusion Our preliminary findings revealed no association between endometriosis and − 460T > C, − 1154G > A, + 936C > T and + 2578C > A of VEGF genes among Nigerian women.https://doi.org/10.1186/s40246-021-00364-xEndometriosisVascular endothelial growth factorAscitesGenetic polymorphismNigeria |
| spellingShingle | Ochuwa Adiketu Babah Oyesola Oyewole Ojewunmi Akinniyi Adediran Osuntoki Melissa A. Simon Bosede Bukola Afolabi Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women Human Genomics Endometriosis Vascular endothelial growth factor Ascites Genetic polymorphism Nigeria |
| title | Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women |
| title_full | Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women |
| title_fullStr | Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women |
| title_full_unstemmed | Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women |
| title_short | Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women |
| title_sort | genetic polymorphisms of vascular endothelial growth factor vegf associated with endometriosis in nigerian women |
| topic | Endometriosis Vascular endothelial growth factor Ascites Genetic polymorphism Nigeria |
| url | https://doi.org/10.1186/s40246-021-00364-x |
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