Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum
Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor a...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-02-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016417302062 |
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| author | Fatmah A.S. Alasmary Amani S. Awaad Mehnaz Kamal Saleh I. Alqasoumi Mohamed E. Zain |
| author_facet | Fatmah A.S. Alasmary Amani S. Awaad Mehnaz Kamal Saleh I. Alqasoumi Mohamed E. Zain |
| author_sort | Fatmah A.S. Alasmary |
| collection | DOAJ |
| description | Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma), HeLa (cervical carcinoma), HEp-2 (larynx carcinoma) and HepG-2 (hepatocellular carcinoma) cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ± 0.1 to 217.0 ± 0.3 µg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5 ± 0.3, 059.0 ± 0.5 and 059.0 ± 0.1 for HEp-2, Hela, and HepG-2 respectively). The isolated compounds (H1&H2) were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5–20.3 μg/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells. |
| first_indexed | 2024-12-12T08:31:57Z |
| format | Article |
| id | doaj.art-03e1f00cc97e487e87fd9239aacc2a4a |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-12T08:31:57Z |
| publishDate | 2018-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-03e1f00cc97e487e87fd9239aacc2a4a2022-12-22T00:31:03ZengElsevierSaudi Pharmaceutical Journal1319-01642018-02-0126227928510.1016/j.jsps.2017.11.011Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilumFatmah A.S. Alasmary0Amani S. Awaad1Mehnaz Kamal2Saleh I. Alqasoumi3Mohamed E. Zain4Chemistry Department, College of Science, King Saud University, Riyadh 11362, Saudi ArabiaPharmacognosy Department, College of Pharmacy, Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaPharmacognosy Department, College of Pharmacy, King Saud University, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, EgyptTotal extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma), HeLa (cervical carcinoma), HEp-2 (larynx carcinoma) and HepG-2 (hepatocellular carcinoma) cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ± 0.1 to 217.0 ± 0.3 µg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5 ± 0.3, 059.0 ± 0.5 and 059.0 ± 0.1 for HEp-2, Hela, and HepG-2 respectively). The isolated compounds (H1&H2) were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5–20.3 μg/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells.http://www.sciencedirect.com/science/article/pii/S1319016417302062Drechslera rostrataEurotium tonophilumDi-2-ethylhexyl phthalate1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinoneMTT assayHuman cancer cell lines |
| spellingShingle | Fatmah A.S. Alasmary Amani S. Awaad Mehnaz Kamal Saleh I. Alqasoumi Mohamed E. Zain Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum Saudi Pharmaceutical Journal Drechslera rostrata Eurotium tonophilum Di-2-ethylhexyl phthalate 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone MTT assay Human cancer cell lines |
| title | Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum |
| title_full | Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum |
| title_fullStr | Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum |
| title_full_unstemmed | Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum |
| title_short | Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum |
| title_sort | antitumor activity of extract and isolated compounds from drechslera rostrata and eurotium tonophilum |
| topic | Drechslera rostrata Eurotium tonophilum Di-2-ethylhexyl phthalate 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone MTT assay Human cancer cell lines |
| url | http://www.sciencedirect.com/science/article/pii/S1319016417302062 |
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