Volatile organic compounds associated with Plasmodium falciparum infection in vitro
Abstract Background In order to identify new ways to prevent transmission of vector-borne diseases such as malaria, efforts have been made to understand how insects are attracted to humans. Vector-host interaction studies have shown that several volatile compounds play an important role in attractin...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2157-x |
| _version_ | 1811293370739326976 |
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| author | Ricardo Correa Lorena M. Coronado Anette C. Garrido Armando A. Durant-Archibold Carmenza Spadafora |
| author_facet | Ricardo Correa Lorena M. Coronado Anette C. Garrido Armando A. Durant-Archibold Carmenza Spadafora |
| author_sort | Ricardo Correa |
| collection | DOAJ |
| description | Abstract Background In order to identify new ways to prevent transmission of vector-borne diseases such as malaria, efforts have been made to understand how insects are attracted to humans. Vector-host interaction studies have shown that several volatile compounds play an important role in attracting mosquitoes to human targets. A headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HSPME GC-MS) analysis of the volatile organic composition of extracellular vesicles (EVs) and supernatants of ultracentrifugation (SNUs) was carried out in Plasmodium falciparum-infected cultures with high and low parasitemias. Results A list of 18 volatile organic compounds (VOCs) was obtained from the EVs of both infected and uninfected RBCs with 1,2,3-Propanetriol, diacetate (diacetin) increased in the infected EVs, regardless of the parasitemia of the culture. The supernatant analysis, however, gave off 56 VOCs, with pentane 2,2,4-trimethyl being present in all the SNUs of uninfected erythrocytes but absent from the parasite-infected ones. Standing out in this study was hexanal, a reported insect attractant, which was the only VOC present in all samples from SNUs from infected erythrocytes and absent from uninfected ones, suggesting that it originates during parasite infection. Conclusions The hexanal compound, reportedly a low-level component found in healthy human samples such as breath and plasma, had not been found in previous analyses of P. falciparum-infected patients or cultures. This compound has been reported as an Anopheles gambiae attractant in plants. While the compound could be produced during infection by the malaria parasite in human erythrocytes, the A. gambiae attraction could be used by the parasite as a strategy for transmission. |
| first_indexed | 2024-04-13T05:00:24Z |
| format | Article |
| id | doaj.art-03e29055732d400185469e3f0980e079 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T05:00:24Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-03e29055732d400185469e3f0980e0792022-12-22T03:01:21ZengBMCParasites & Vectors1756-33052017-05-011011810.1186/s13071-017-2157-xVolatile organic compounds associated with Plasmodium falciparum infection in vitroRicardo Correa0Lorena M. Coronado1Anette C. Garrido2Armando A. Durant-Archibold3Carmenza Spadafora4Center of Cellular and Molecular Biology of Diseases (CBCMe), Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)Center of Cellular and Molecular Biology of Diseases (CBCMe), Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)Molecular Medicine Research Unit, Center for Biodiversity and Drug Discovery, Instituto de Investigaciones Cientificas y Servicios de Alta Tecnologia (INDICASAT AIP)Molecular Medicine Research Unit, Center for Biodiversity and Drug Discovery, Instituto de Investigaciones Cientificas y Servicios de Alta Tecnologia (INDICASAT AIP)Center of Cellular and Molecular Biology of Diseases (CBCMe), Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)Abstract Background In order to identify new ways to prevent transmission of vector-borne diseases such as malaria, efforts have been made to understand how insects are attracted to humans. Vector-host interaction studies have shown that several volatile compounds play an important role in attracting mosquitoes to human targets. A headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HSPME GC-MS) analysis of the volatile organic composition of extracellular vesicles (EVs) and supernatants of ultracentrifugation (SNUs) was carried out in Plasmodium falciparum-infected cultures with high and low parasitemias. Results A list of 18 volatile organic compounds (VOCs) was obtained from the EVs of both infected and uninfected RBCs with 1,2,3-Propanetriol, diacetate (diacetin) increased in the infected EVs, regardless of the parasitemia of the culture. The supernatant analysis, however, gave off 56 VOCs, with pentane 2,2,4-trimethyl being present in all the SNUs of uninfected erythrocytes but absent from the parasite-infected ones. Standing out in this study was hexanal, a reported insect attractant, which was the only VOC present in all samples from SNUs from infected erythrocytes and absent from uninfected ones, suggesting that it originates during parasite infection. Conclusions The hexanal compound, reportedly a low-level component found in healthy human samples such as breath and plasma, had not been found in previous analyses of P. falciparum-infected patients or cultures. This compound has been reported as an Anopheles gambiae attractant in plants. While the compound could be produced during infection by the malaria parasite in human erythrocytes, the A. gambiae attraction could be used by the parasite as a strategy for transmission.http://link.springer.com/article/10.1186/s13071-017-2157-xVolatile organic compoundsHSPMEGC-MSPlasmodium falciparumMalariaVector |
| spellingShingle | Ricardo Correa Lorena M. Coronado Anette C. Garrido Armando A. Durant-Archibold Carmenza Spadafora Volatile organic compounds associated with Plasmodium falciparum infection in vitro Parasites & Vectors Volatile organic compounds HSPME GC-MS Plasmodium falciparum Malaria Vector |
| title | Volatile organic compounds associated with Plasmodium falciparum infection in vitro |
| title_full | Volatile organic compounds associated with Plasmodium falciparum infection in vitro |
| title_fullStr | Volatile organic compounds associated with Plasmodium falciparum infection in vitro |
| title_full_unstemmed | Volatile organic compounds associated with Plasmodium falciparum infection in vitro |
| title_short | Volatile organic compounds associated with Plasmodium falciparum infection in vitro |
| title_sort | volatile organic compounds associated with plasmodium falciparum infection in vitro |
| topic | Volatile organic compounds HSPME GC-MS Plasmodium falciparum Malaria Vector |
| url | http://link.springer.com/article/10.1186/s13071-017-2157-x |
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