Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion
To date, there have been three common methods for sampling the cerebral ischemic border zone in a rat model of transient middle cerebral artery occlusion (tMCAO): the “two o'clock method”, the “diagonal method”, and the “parallel line method”. However, these methods have their own advantages an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2023-12-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100695&lng=en&tlng=en |
| _version_ | 1797394250125541376 |
|---|---|
| author | Sui-yi Xu Mao-mei Song Xue Pan Shi-na Song Qi Zhang Chang-xin Li |
| author_facet | Sui-yi Xu Mao-mei Song Xue Pan Shi-na Song Qi Zhang Chang-xin Li |
| author_sort | Sui-yi Xu |
| collection | DOAJ |
| description | To date, there have been three common methods for sampling the cerebral ischemic border zone in a rat model of transient middle cerebral artery occlusion (tMCAO): the “two o'clock method”, the “diagonal method”, and the “parallel line method”. However, these methods have their own advantages and limitations. Here, we propose a modified technique (the “rectangular method“) for sampling the ischemic border zone. A rat tMCAO model was prepared under the support of a compact small animal anesthesia machine. Cerebral blood flow was monitored by high-resolution laser Doppler to control the quality of modeling, and 2,3,5-triphenyl tetrazolium chloride (TTC) staining was used for cerebral infarction location assessment. Superoxide dismutase 2 (SOD2), cysteinyl aspartate specific proteinase (caspase)-3, caspase-9, and heat shock protein 70 (HSP70) were used to verify the reliability and reproducibility of the rectangular method. The expression of biomarkers (SOD2, caspase-3, caspase-9, and HSP70) in the traditional (two o'clock method after TTC staining) and modified (rectangular method) groups were increased. There were no significant differences between the groups. The rectangular method proposed herein is based on a modification of the diagonal method and parallel line method, which could provide a directly observable infarct borderline and a sufficient sampling area for subsequent experimental operations regardless of the cerebral infarct location. The assessed biomarkers (SOD2, caspase-3, caspase-9, and HSP70) demonstrated the reliability and reproducibility of the rectangular method, which may facilitate inter-laboratory comparisons. |
| first_indexed | 2024-03-09T00:15:46Z |
| format | Article |
| id | doaj.art-03e3d3662cd349cd99710258baa40a8e |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-09T00:15:46Z |
| publishDate | 2023-12-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-03e3d3662cd349cd99710258baa40a8e2023-12-12T07:52:17ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2023-12-015610.1590/1414-431x2023e13140Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusionSui-yi Xuhttps://orcid.org/0000-0002-8908-011XMao-mei Songhttps://orcid.org/0000-0002-9288-8726Xue Panhttps://orcid.org/0000-0002-1471-3840Shi-na Songhttps://orcid.org/0009-0009-6177-2518Qi Zhanghttps://orcid.org/0000-0003-2448-0275Chang-xin Lihttps://orcid.org/0000-0001-8652-4283To date, there have been three common methods for sampling the cerebral ischemic border zone in a rat model of transient middle cerebral artery occlusion (tMCAO): the “two o'clock method”, the “diagonal method”, and the “parallel line method”. However, these methods have their own advantages and limitations. Here, we propose a modified technique (the “rectangular method“) for sampling the ischemic border zone. A rat tMCAO model was prepared under the support of a compact small animal anesthesia machine. Cerebral blood flow was monitored by high-resolution laser Doppler to control the quality of modeling, and 2,3,5-triphenyl tetrazolium chloride (TTC) staining was used for cerebral infarction location assessment. Superoxide dismutase 2 (SOD2), cysteinyl aspartate specific proteinase (caspase)-3, caspase-9, and heat shock protein 70 (HSP70) were used to verify the reliability and reproducibility of the rectangular method. The expression of biomarkers (SOD2, caspase-3, caspase-9, and HSP70) in the traditional (two o'clock method after TTC staining) and modified (rectangular method) groups were increased. There were no significant differences between the groups. The rectangular method proposed herein is based on a modification of the diagonal method and parallel line method, which could provide a directly observable infarct borderline and a sufficient sampling area for subsequent experimental operations regardless of the cerebral infarct location. The assessed biomarkers (SOD2, caspase-3, caspase-9, and HSP70) demonstrated the reliability and reproducibility of the rectangular method, which may facilitate inter-laboratory comparisons.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100695&lng=en&tlng=enStrokeAnimal modelMiddle cerebral artery occlusion |
| spellingShingle | Sui-yi Xu Mao-mei Song Xue Pan Shi-na Song Qi Zhang Chang-xin Li Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion Brazilian Journal of Medical and Biological Research Stroke Animal model Middle cerebral artery occlusion |
| title | Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| title_full | Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| title_fullStr | Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| title_full_unstemmed | Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| title_short | Rectangular method: a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| title_sort | rectangular method a modified technique for sampling the ischemic border zone in a rat model of transient middle cerebral artery occlusion |
| topic | Stroke Animal model Middle cerebral artery occlusion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100695&lng=en&tlng=en |
| work_keys_str_mv | AT suiyixu rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion AT maomeisong rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion AT xuepan rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion AT shinasong rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion AT qizhang rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion AT changxinli rectangularmethodamodifiedtechniqueforsamplingtheischemicborderzoneinaratmodeloftransientmiddlecerebralarteryocclusion |