Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study
Introduction: Micrometastasis to the lymph node is an important prognostic factor in survival and recurrence and even in making critical decisions regarding postoperative radiation treatment. Methods like Immunohistochemistry (IHC), serial sectioning and Polymerase Chain Reaction (PCR) are very...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9044/20262_CE(Ra1)_F(AK)_PF1(PG_RK)_PFA(AK)_PF2(EKAK).pdf |
| Summary: | Introduction: Micrometastasis to the lymph node is an important
prognostic factor in survival and recurrence and even in making
critical decisions regarding postoperative radiation treatment.
Methods like Immunohistochemistry (IHC), serial sectioning and
Polymerase Chain Reaction (PCR) are very sensitive; however,
they are expensive and time consuming. Hence, there is a need
for the detection of micrometastasis by method which is easily
feasible and inexpensive.
Aim: This study was undertaken to identify if special stains
(Modified Papanicolaou stain and Ayoub Shklar stain) are
valuable in detecting micrometastasis which are routinely
missed in Haematoxylin and Eosin stain and also to compare
their efficacy over Haematoxylin and Eosin stain in detection of
metastatic tumour cells in non-metastatic lymph nodes.
Materials and Methods: This study constituted a total of
300 histopathologically proven non metastatic lymph node
sections. Other than Haematoxylin and Eosin stain, Modified
Papanicalaou and Ayoub Shklar stain were used for identification
of micrometastatic deposit.
Results: Papanicolaou stain was the only stain useful in
detecting micrometastasis which accounted to 7% of non
metastatic lymph node sections used in our study.
Conclusion: Papanicolaou stain has definitely proved valuable
in detecting micrometastasis over routine Haematoxylin and
Eosin and Ayoub Shklar stain with an added advantage of being
economical, easily available and technique insensitive. |
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| ISSN: | 2249-782X 0973-709X |