Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study
Introduction: Micrometastasis to the lymph node is an important prognostic factor in survival and recurrence and even in making critical decisions regarding postoperative radiation treatment. Methods like Immunohistochemistry (IHC), serial sectioning and Polymerase Chain Reaction (PCR) are very...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9044/20262_CE(Ra1)_F(AK)_PF1(PG_RK)_PFA(AK)_PF2(EKAK).pdf |
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| author | Wasim Raja Mumtaz Veda Hegde Nikhil Yadav |
| author_facet | Wasim Raja Mumtaz Veda Hegde Nikhil Yadav |
| author_sort | Wasim Raja Mumtaz |
| collection | DOAJ |
| description | Introduction: Micrometastasis to the lymph node is an important
prognostic factor in survival and recurrence and even in making
critical decisions regarding postoperative radiation treatment.
Methods like Immunohistochemistry (IHC), serial sectioning and
Polymerase Chain Reaction (PCR) are very sensitive; however,
they are expensive and time consuming. Hence, there is a need
for the detection of micrometastasis by method which is easily
feasible and inexpensive.
Aim: This study was undertaken to identify if special stains
(Modified Papanicolaou stain and Ayoub Shklar stain) are
valuable in detecting micrometastasis which are routinely
missed in Haematoxylin and Eosin stain and also to compare
their efficacy over Haematoxylin and Eosin stain in detection of
metastatic tumour cells in non-metastatic lymph nodes.
Materials and Methods: This study constituted a total of
300 histopathologically proven non metastatic lymph node
sections. Other than Haematoxylin and Eosin stain, Modified
Papanicalaou and Ayoub Shklar stain were used for identification
of micrometastatic deposit.
Results: Papanicolaou stain was the only stain useful in
detecting micrometastasis which accounted to 7% of non
metastatic lymph node sections used in our study.
Conclusion: Papanicolaou stain has definitely proved valuable
in detecting micrometastasis over routine Haematoxylin and
Eosin and Ayoub Shklar stain with an added advantage of being
economical, easily available and technique insensitive. |
| first_indexed | 2024-12-13T05:08:58Z |
| format | Article |
| id | doaj.art-03eaaa8cd57d47219fd900e9ff60aa74 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T05:08:58Z |
| publishDate | 2016-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-03eaaa8cd57d47219fd900e9ff60aa742022-12-21T23:58:35ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-12-011012ZC23ZC2610.7860/JCDR/2016/20262.9044Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical StudyWasim Raja Mumtaz0Veda Hegde1Nikhil Yadav2Postgraduate Student, Department of Oral Pathology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India.Professor, Department of Oral Pathology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India.Postgraduate Student, Department of Oral Pathology, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India.Introduction: Micrometastasis to the lymph node is an important prognostic factor in survival and recurrence and even in making critical decisions regarding postoperative radiation treatment. Methods like Immunohistochemistry (IHC), serial sectioning and Polymerase Chain Reaction (PCR) are very sensitive; however, they are expensive and time consuming. Hence, there is a need for the detection of micrometastasis by method which is easily feasible and inexpensive. Aim: This study was undertaken to identify if special stains (Modified Papanicolaou stain and Ayoub Shklar stain) are valuable in detecting micrometastasis which are routinely missed in Haematoxylin and Eosin stain and also to compare their efficacy over Haematoxylin and Eosin stain in detection of metastatic tumour cells in non-metastatic lymph nodes. Materials and Methods: This study constituted a total of 300 histopathologically proven non metastatic lymph node sections. Other than Haematoxylin and Eosin stain, Modified Papanicalaou and Ayoub Shklar stain were used for identification of micrometastatic deposit. Results: Papanicolaou stain was the only stain useful in detecting micrometastasis which accounted to 7% of non metastatic lymph node sections used in our study. Conclusion: Papanicolaou stain has definitely proved valuable in detecting micrometastasis over routine Haematoxylin and Eosin and Ayoub Shklar stain with an added advantage of being economical, easily available and technique insensitive.https://jcdr.net/articles/PDF/9044/20262_CE(Ra1)_F(AK)_PF1(PG_RK)_PFA(AK)_PF2(EKAK).pdfhaematoxylin and eosin stainlymph nodesmetastasispapanicoloaou stain |
| spellingShingle | Wasim Raja Mumtaz Veda Hegde Nikhil Yadav Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study Journal of Clinical and Diagnostic Research haematoxylin and eosin stain lymph nodes metastasis papanicoloaou stain |
| title | Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study |
| title_full | Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study |
| title_fullStr | Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study |
| title_full_unstemmed | Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study |
| title_short | Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma – A Histochemical Study |
| title_sort | micrometastasis detection using special stains in nodal tissues of oral squamous cell carcinoma a histochemical study |
| topic | haematoxylin and eosin stain lymph nodes metastasis papanicoloaou stain |
| url | https://jcdr.net/articles/PDF/9044/20262_CE(Ra1)_F(AK)_PF1(PG_RK)_PFA(AK)_PF2(EKAK).pdf |
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