Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs
Abstract This study examined the effects of maternal and/or post-weaning Bacillus altitudinis supplementation on the microbiota in sow colostrum and faeces, and offspring digesta and faeces. Sows (n = 12/group) were assigned to: (1) standard diet (CON), or (2) CON supplemented with probiotic B. alti...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33175-2 |
| _version_ | 1827933451942100992 |
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| author | Ruth Rattigan Peadar G. Lawlor Paul Cormican Daniel Crespo-Piazuelo James Cullen John P. Phelan Samir Ranjitkar Fiona Crispie Gillian E. Gardiner |
| author_facet | Ruth Rattigan Peadar G. Lawlor Paul Cormican Daniel Crespo-Piazuelo James Cullen John P. Phelan Samir Ranjitkar Fiona Crispie Gillian E. Gardiner |
| author_sort | Ruth Rattigan |
| collection | DOAJ |
| description | Abstract This study examined the effects of maternal and/or post-weaning Bacillus altitudinis supplementation on the microbiota in sow colostrum and faeces, and offspring digesta and faeces. Sows (n = 12/group) were assigned to: (1) standard diet (CON), or (2) CON supplemented with probiotic B. altitudinis spores (PRO) from day (d)100 of gestation to weaning (d26 of lactation). At weaning, offspring were assigned to CON or PRO for 28d, resulting in: (1) CON/CON, (2) CON/PRO, (3) PRO/CON, and (4) PRO/PRO, after which all received CON. Samples were collected from sows and selected offspring (n = 10/group) for 16S rRNA gene sequencing. Rothia was more abundant in PRO sow colostrum. Sow faeces were not impacted but differences were identified in offspring faeces and digesta. Most were in the ileal digesta between PRO/CON and CON/CON on d8 post-weaning; i.e. Bacteroidota, Alloprevotella, Prevotella, Prevotellaceae, Turicibacter, Catenibacterium and Blautia were more abundant in PRO/CON, with Firmicutes and Blautia more abundant in PRO/PRO compared with CON/CON. Lactobacillus was more abundant in PRO/CON faeces on d118 post-weaning. This increased abundance of polysaccharide-fermenters (Prevotella, Alloprevotella, Prevotellaceae), butyrate-producers (Blautia) and Lactobacillus likely contributed to previously reported improvements in growth performance. Overall, maternal, rather than post-weaning, probiotic supplementation had the greatest impact on intestinal microbiota. |
| first_indexed | 2024-03-13T07:24:55Z |
| format | Article |
| id | doaj.art-03eb3f19efc14f3e9d4127e2348891af |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T07:24:55Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-03eb3f19efc14f3e9d4127e2348891af2023-06-04T11:25:21ZengNature PortfolioScientific Reports2045-23222023-06-0113111810.1038/s41598-023-33175-2Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigsRuth Rattigan0Peadar G. Lawlor1Paul Cormican2Daniel Crespo-Piazuelo3James Cullen4John P. Phelan5Samir Ranjitkar6Fiona Crispie7Gillian E. Gardiner8Eco-Innovation Research Centre, Department of Science, Waterford Campus, South East Technological UniversityPig Development Department, Animal and Grassland Research and Innovation Centre, TeagascAnimal and Grassland Research and Innovation Centre, TeagascPig Development Department, Animal and Grassland Research and Innovation Centre, TeagascEco-Innovation Research Centre, Department of Science, Waterford Campus, South East Technological UniversityEco-Innovation Research Centre, Department of Science, Waterford Campus, South East Technological UniversityPig Development Department, Animal and Grassland Research and Innovation Centre, TeagascAPC Microbiome Ireland, University College CorkEco-Innovation Research Centre, Department of Science, Waterford Campus, South East Technological UniversityAbstract This study examined the effects of maternal and/or post-weaning Bacillus altitudinis supplementation on the microbiota in sow colostrum and faeces, and offspring digesta and faeces. Sows (n = 12/group) were assigned to: (1) standard diet (CON), or (2) CON supplemented with probiotic B. altitudinis spores (PRO) from day (d)100 of gestation to weaning (d26 of lactation). At weaning, offspring were assigned to CON or PRO for 28d, resulting in: (1) CON/CON, (2) CON/PRO, (3) PRO/CON, and (4) PRO/PRO, after which all received CON. Samples were collected from sows and selected offspring (n = 10/group) for 16S rRNA gene sequencing. Rothia was more abundant in PRO sow colostrum. Sow faeces were not impacted but differences were identified in offspring faeces and digesta. Most were in the ileal digesta between PRO/CON and CON/CON on d8 post-weaning; i.e. Bacteroidota, Alloprevotella, Prevotella, Prevotellaceae, Turicibacter, Catenibacterium and Blautia were more abundant in PRO/CON, with Firmicutes and Blautia more abundant in PRO/PRO compared with CON/CON. Lactobacillus was more abundant in PRO/CON faeces on d118 post-weaning. This increased abundance of polysaccharide-fermenters (Prevotella, Alloprevotella, Prevotellaceae), butyrate-producers (Blautia) and Lactobacillus likely contributed to previously reported improvements in growth performance. Overall, maternal, rather than post-weaning, probiotic supplementation had the greatest impact on intestinal microbiota.https://doi.org/10.1038/s41598-023-33175-2 |
| spellingShingle | Ruth Rattigan Peadar G. Lawlor Paul Cormican Daniel Crespo-Piazuelo James Cullen John P. Phelan Samir Ranjitkar Fiona Crispie Gillian E. Gardiner Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs Scientific Reports |
| title | Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs |
| title_full | Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs |
| title_fullStr | Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs |
| title_full_unstemmed | Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs |
| title_short | Maternal and/or post-weaning supplementation with Bacillus altitudinis spores modulates the microbial composition of colostrum, digesta and faeces in pigs |
| title_sort | maternal and or post weaning supplementation with bacillus altitudinis spores modulates the microbial composition of colostrum digesta and faeces in pigs |
| url | https://doi.org/10.1038/s41598-023-33175-2 |
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